Removing sequencing migration artefacts (View forum version)



DavidS

Posted 13 April 2010 - 06:39 AM

Has anyone had success using additives in sanger sequencing reactions to remove sequence fragment migration anomalies/artefacts

mdfenko

Posted 15 April 2010 - 12:03 PM

what kind of artifacts?

what are you seeing?

on what are you visualizing the reaction (machine, gel,...)?

some standard pcr additives (eg- betaine, ethylene glycol, propylene glycol, dmso, etc) work with sequencing difficult templates.