western (View forum version)



ulujm

Posted 12 November 2009 - 09:40 AM

Hi in non reducing condition my actin signal is low (alpha actine)

any idea why? is because it's in non reducing condition?

beta actin is it better?

bob1

Posted 12 November 2009 - 03:06 PM

Possibly, it would depend on your antibody as to whether the epitope is exposed under non-reducing conditions.