Small flourescent tag (View forum version)


Posted 01 November 2009 - 11:47 PM


Just wondering if anyone is familar with a small flouresent tag which may be used to track a protein using real-time microscopy techniques?

I am finishing my honours project and i have found that EGFP affects the localization of my protein of interest (its much larger). I used the FLAG tag in my localization expriments. However, this tag is not appropriate for live cell imaging (atleast i dont think so)... as it needs an anti-body for detection. I am just wanting to make some recommendations for future directions of the project... and web searches have been unsucessful.

Any hints or ideas would be great.



Posted 02 December 2009 - 04:02 PM

it comes to my mind HaloTag from Promega
on academic settings, prof K. Johnsson at EPFL Lausanne is making breakthroughs with new tag technologies for proteins.

---my blog on genetically-encoded methods----