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Pangea's Content

There have been 97 items by Pangea (Search limited from 10-April 20)



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#147858 what is the contamination in buffers

Posted by Pangea on 11 January 2013 - 06:59 AM in General Lab Techniques

Are the buffers bought from a company and is there SDS inside? One PhD in our Lab changed the whole Kit because she was not able to read. How stuipid, isnt? Generally, most buffer are store in RT. If there is contamination then it is quite a old solution. Try to for cell culture always aotuclaving the solution and the others in the Lab as you know wiht ddH2O and store tightly closed. Make new once if frequently used. To my knowledge like TAE or TBE or Running Buffer you can make stock solutions so there is no chance to growth for contaminants. And its not specific for TRIS its just a matter of handling your solution. If i was able to help you.



#147859 What is Ks?

Posted by Pangea on 11 January 2013 - 07:00 AM in General Lab Techniques

Best answer give you google, isnt?



#147908 Wet transfer of proteins between 100-200kDa

Posted by Pangea on 12 January 2013 - 12:01 PM in SDS-PAGE and Western Blotting

I think 120V for 1.30 - 2.00 h. Maybe should put PVDF in methanol first before transferring. Always check your protein ladder/marker.



#148092 western blot troubleshooting

Posted by Pangea on 15 January 2013 - 08:26 AM in Molecular Biology

Welcome.

as almost says, we need more detail and specification of your problem. But it seems that you just have different amount of actin as a internal control. Thats because your loading conc. are different when you perform SDS-PAGE.You can calculate the amount maybe with densitometric methods.



#147501 TRYPSIN

Posted by Pangea on 05 January 2013 - 02:13 AM in Molecular Cloning

Thanks for your Advice.



#147493 TRYPSIN

Posted by Pangea on 05 January 2013 - 12:52 AM in Molecular Cloning

To make it more cheaper ;). Dou you have an idea? Maybe i will get the clone from IMAGE Consortium. Or get the trypsin from slaughterhouse.



#147481 TRYPSIN

Posted by Pangea on 04 January 2013 - 02:51 PM in Molecular Cloning

How can i produce Cell culture Trypsin? Any Idea?



#147941 TAP Water

Posted by Pangea on 13 January 2013 - 06:23 AM in General Lab Techniques

It will be serious check. Cool manual.



#147918 TAP Water

Posted by Pangea on 12 January 2013 - 01:36 PM in General Lab Techniques

How can I analyze tap water for microbes?



#147946 Suggestion for a book!

Posted by Pangea on 13 January 2013 - 06:49 AM in Immunology

Listen type into google search as follows: Cytokine filetype:pdf

The part filetype will give you just PDF files and gives you a smile in your face:). You can do this also with doc, xls, etc. Enjoy the discovery.


Books:
Cytokine Protocols (Methods in Molecular Biology)

http://elib.fk.uwks....ogy Series].pdf

http://www.ctf.edu.t...1_Cytokines.pdf



#147948 Stem Cell Donation

Posted by Pangea on 13 January 2013 - 07:40 AM in Stem Cell

Where can I type me and donate stem cells?



#147963 Stem Cell Donation

Posted by Pangea on 13 January 2013 - 09:40 AM in Stem Cell

Cheers on both.



#147957 Stem Cell Donation

Posted by Pangea on 13 January 2013 - 09:28 AM in Stem Cell

Germany



#147510 Spin Column

Posted by Pangea on 05 January 2013 - 05:21 AM in General Lab Techniques

Cheers very helpful.



#147502 Spin Column

Posted by Pangea on 05 January 2013 - 02:18 AM in General Lab Techniques

Where can i get single spin columns for miniprep?



#147949 Software for scanning of specific aa sequence in proteins

Posted by Pangea on 13 January 2013 - 07:45 AM in Bioinformatics and Biostatistics

Check this:

http://blast.ncbi.nlm.nih.gov/



#147905 Software for designing cell pathways

Posted by Pangea on 12 January 2013 - 11:41 AM in Bioinformatics and Biostatistics

I think people are doing for there own signalling pathway by Illustrator. But that takes a liitle bit time. Maybe this website will help you:



http://string-db.org/

http://www.cellsigna...hway/index.html



#147494 siRNA in E.Coli

Posted by Pangea on 05 January 2013 - 12:57 AM in siRNA, microRNA and RNAi

Sounds good.Thanks.



#147447 siRNA in E.Coli

Posted by Pangea on 04 January 2013 - 10:23 AM in siRNA, microRNA and RNAi

Do E.coli have siRNA Metabolism?



#147637 Restriction Enzymes

Posted by Pangea on 07 January 2013 - 01:26 PM in Molecular Biology

Is it possible to get the genes of the general used Restriction Enzymes from NCBI?



#147660 Restriction Enzymes

Posted by Pangea on 08 January 2013 - 01:56 AM in Molecular Biology

Ok , lets say i want gene sequence of an RE from IMAGE consortium. And produce my own RE in E.coli. I am no considering the cost and sso on.. . Question is if its practcally possible in the LAB.



#147380 Residues on the N terminal GST Tag

Posted by Pangea on 03 January 2013 - 09:10 AM in Molecular Cloning

İ have a stupid question. İf i clone a HİS tag directly to my rProtein İ have no Amino Acid inbetween. But in case of GST Tag İ am getting additionally AA to my rProtein. Am İ right? How can İ avoid this.

Best regards



#147548 Replication of the Plasmid DNA

Posted by Pangea on 05 January 2013 - 03:25 PM in Genetics and Genomics

You mean the Replication machinary.



#147495 Replication of the Plasmid DNA

Posted by Pangea on 05 January 2013 - 01:01 AM in Genetics and Genomics

My Question would be which enzyme do we need in replicating Plasmids in E.coli? Any idea.



#147665 Replication of the Plasmid DNA

Posted by Pangea on 08 January 2013 - 07:24 AM in Genetics and Genomics

:)




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