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rajaram's Content

There have been 13 items by rajaram (Search limited from 23-February 19)


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#69218 Antiglycolyte

Posted by rajaram on 03 May 2010 - 05:43 PM in Biochemistry

Hi,
Flouride and Iodoacetate, which one is effective antiglycolyte? Both works at 3C...once 6C (hexose) has been bifurcated to 3C's, how glycolysis could be stoped and how we say glucose in our blood sample is undepleted under these inhibitors ?



#69166 post hoc test

Posted by rajaram on 03 May 2010 - 09:03 AM in Bioinformatics and Biostatistics

Thanks



#69142 post hoc test

Posted by rajaram on 03 May 2010 - 06:39 AM in Bioinformatics and Biostatistics

Hi,
Can anyone explain me why there are many tests (Duncan, Tukey, Bonferroni etc.,) for Post hoc analysis??? Which one is most suitable for biological research?
I could find Duncan's post hoc in majority of research articles, while few were used Tukey's...?



#68038 what's wrong with my anthrone reagent?

Posted by rajaram on 24 April 2010 - 06:29 PM in Biochemistry

Hi there,
When you're preparing anthrone under ice cold condition the colour will be same as you are getting.

Brown colour possibly...
1.Preparing antrone reagent under room temperatue
2.Not giving sufficient time for cooling of freshly prepared 80%H2SO4
3.Anthrone might've oxidized

for any reagents temperature, oxygen and light are crucial.



#28226 Temperature vs pH

Posted by rajaram on 03 July 2009 - 09:27 AM in Biochemistry

Hi Pito,
Dissociation matters...
Thank you very much



#27823 Temperature vs pH

Posted by rajaram on 29 June 2009 - 06:07 AM in Biochemistry

Hi,
How does temperature affect the pH of liquids?
Is it possible to prepare a buffer of high pH stablility?



#24133 Extinction coefficient

Posted by rajaram on 12 May 2009 - 11:44 AM in Protein and Proteomics

May be the %extinction coefficient 2.83 is wrong. Instead it would have been 2.33, if so, the answer is 17.2. By formula 2.83 gives 21.0. you are right.



#24118 Extinction coefficient

Posted by rajaram on 12 May 2009 - 08:43 AM in Biochemistry

Thanks, Its very useful links.



#24117 Extinction coefficient

Posted by rajaram on 12 May 2009 - 08:39 AM in Biochemistry

Hi,
Sorry, its like this "molar extinction coefficient at 280 nm for BSA is approximately 43,824 M-1 cm-1"



#24008 Extinction coefficient

Posted by rajaram on 11 May 2009 - 06:38 AM in Biochemistry

DRN,
Thanks. 43824 is molar extinction coefficient of BSA at A280. how to find 43824?



#23941 Extinction coefficient

Posted by rajaram on 10 May 2009 - 03:48 PM in Protein and Proteomics

Dear Mr, Gerard,

Thank you very much for your prompt reply.
The following lines i've just copied from a Scientific paper, in this i could not understand E17.26 from 2.83...

"hemocyanin concentration was calculated using an extinction coefficient (ΕmM1cm) of 17.26, calculated from (E1%1cm) = 2.83 (Nickerson & Van Holde 1971) on the basis of a functional subunit of 74 000 (Antonini & Brunori 1974)."



#23847 Extinction coefficient

Posted by rajaram on 08 May 2009 - 06:52 PM in Biochemistry

Hi friends... I have few queries to be solved...
1.How to calculate extinction coefficient for hemocyanin at 335nm?

For example:
hemocyanin concentration was calculated using an extinction coefficient (ΕmM1cm) of 17.26, calculated from (E1%1cm) = 2.83 (Nickerson & Van Holde 1971) on the basis of a functional subunit of 74 000 (Antonini & Brunori 1974).

2.If we have extinction coefficient, How to calculate molar concentration?
3.How consistant is this quantificaiton of biochemical compounds based on extinction coefficient?
I am unacquainted to this UV spectroscopy, please explain with funtamental concepts.
Thank you for reading my query



#23748 Extinction coefficient

Posted by rajaram on 07 May 2009 - 07:27 PM in Protein and Proteomics

Hi friends... I have few queries to be solved...
1.How to calculate extinction coefficient for hemocyanin at 335nm?

For example:
hemocyanin concentration was calculated using an extinction coefficient (ΕmM1cm) of 17.26, calculated from (E1%1cm) = 2.83 (Nickerson & Van Holde 1971) on the basis of a functional subunit of 74 000 (Antonini & Brunori 1974).

2.If we have extinction coefficient, How to calculate molar concentration?
3.How consistant is this quantificaiton of biochemical compounds based on extinction coefficient?
I am unacquainted to this UV spectroscopy, please explain with funtamental concepts.
Thank you for reading my query




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