Hi,
I'm new to the radioassays, and have a few questions.
My assay currently monitors the decarboxylation of 1-14C-pyruvate (where the amount of label is negligible). I assume this is due to the specific activity of the compound increasing as less unlabeled pyruvate is present. And the formation of a pyruvate derivative where the label is present.
So, currently I calculate the amount of pyruvate lost as: Po/Pr (where Po is initial pyruvate added and Pr is the activity of Pyruvate recovered from the assay) is this correct? And the amount of pyruvate derivative gained as a reverse dilution calculation where Pr/Po? Does this seem okay?
Any help will be appreciated,
Thanks
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#20095 Radio assay
Posted by
on 26 March 2009 - 01:27 PM
in
Molecular Biology
#20079 Radiochemical assay
Posted by
on 26 March 2009 - 10:52 AM
in
General Lab Techniques
Hi,
I'm new to the radioassays, and have a few questions.
My assay currently monitors the decarboxylation of 1-14C-pyruvate (where the amount of label is negligible). I assume this is due to the specific activity of the compound increasing as less unlabeled pyruvate is present. And the formation of a pyruvate derivative where the label is present.
So, currently I calculate the amount of pyruvate lost as: Po/Pr (where Po is initial pyruvate added and Pr is the activity of Pyruvate recovered from the assay) is this correct? And the amount of pyruvate derivative gained as a reverse dilution calculation where Pr/Po? Does this seem okay?
Any help will be appreciated,
Thanks,
I'm new to the radioassays, and have a few questions.
My assay currently monitors the decarboxylation of 1-14C-pyruvate (where the amount of label is negligible). I assume this is due to the specific activity of the compound increasing as less unlabeled pyruvate is present. And the formation of a pyruvate derivative where the label is present.
So, currently I calculate the amount of pyruvate lost as: Po/Pr (where Po is initial pyruvate added and Pr is the activity of Pyruvate recovered from the assay) is this correct? And the amount of pyruvate derivative gained as a reverse dilution calculation where Pr/Po? Does this seem okay?
Any help will be appreciated,
Thanks,
