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zhangdagou5858's Content

There have been 4 items by zhangdagou5858 (Search limited from 30-September 19)


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#65299 Help me! How to design nested BSP primers?

Posted by zhangdagou5858 on 03 April 2010 - 10:18 PM in DNA Methylation and Epigenetics

Thanks for your reply,i still have one questions,netsted BSP:1st set primers for original DNA sequence or modified sequence? second set primers for what?



#33414 Help me! How to design nested BSP primers?

Posted by zhangdagou5858 on 17 August 2009 - 11:44 PM in DNA Methylation and Epigenetics

Thanks for your reply, but i can't really know the proccedure of primer design as you told me, you mean design one pair primer and then design another one(inner)? which software do you use? methyl express? I really hope that you take an example to illustrate the process! Thx again for your kind reply!



#33009 Help me! How to design nested BSP primers?

Posted by zhangdagou5858 on 14 August 2009 - 07:02 AM in DNA Methylation and Epigenetics

PCRMAN and methynick, please help.
I am now doing nested BSP, but i don't know how to design nested BSP primer, can you take an example to explain nested BSP primer design? So many people are willing to know the method, it is kindhearted for you to do so! :)



#22419 BSP,MSP primer design?

Posted by zhangdagou5858 on 21 April 2009 - 07:32 PM in DNA Methylation and Epigenetics

I am newbile in methylation, I have an easy question in bsp and msp primer design, how can i design primer for methylation? can i use software methprimer or methyl primer express? which is better in designing primer? please tell me how to design primer in detail, pcrman,please writedown your detailed procedure because there are so many novice here , thank you




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