Thanks for your reply, but i can't really know the proccedure of primer design as you told me, you mean design one pair primer and then design another one(inner)? which software do you use? methyl express? I really hope that you take an example to illustrate the process! Thx again for your kind reply!
PCRMAN and methynick, please help.
I am now doing nested BSP, but i don't know how to design nested BSP primer, can you take an example to explain nested BSP primer design? So many people are willing to know the method, it is kindhearted for you to do so!
I am newbile in methylation, I have an easy question in bsp and msp primer design, how can i design primer for methylation? can i use software methprimer or methyl primer express? which is better in designing primer? please tell me how to design primer in detail, pcrman,please writedown your detailed procedure because there are so many novice here , thank you