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#16682 M.SssI treatment of DNA

Posted by OSU_LAB_RAT on 23 February 2009 - 04:06 PM in DNA Methylation and Epigenetics

This is my first post and I'm not sure that I've done this correctly, but I have a question about methylation... I want to use M.SssI to treat 10ug of genomic DNA and I'm not sure what volumes to use for the M.SssI, NEB Buffer2, and SAM and how long I should let it incubate?? The NEB protocal gives volumes for 1-4ug of DNA, but I can't find anything for 10ug. Thanks!

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