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Nrelo's Content

There have been 2 items by Nrelo (Search limited from 26-September 19)

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#127330 PhD age

Posted by Nrelo on 19 January 2012 - 04:49 AM in Life as a PhD Student and Postdoc


And I am thinking to get married once I finished the degree

#127329 Anyone familiar with ELISA of cytokines in mouse plasma?

Posted by Nrelo on 19 January 2012 - 04:48 AM in ELISA and Immunoassay

I am new to ELISA and I am going to measure TNF-alpha in mouse plasma (from heparinized blood).

The blood was collected from terminal bleeding and I got about 300~400ul plasma. I am not sure about what diluent I should use for diluting the samples.

Also, does anyone know about the basal level of TNF-alpha (in pg/ml) in mouse plasma when doing ELISA?

Many thanks!

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