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There have been 167 items by molgen (Search limited from 23-October 19)
In my experience, you will have a problem explaining your findings. Even if they work, the accuracy and accountability of the AB are compromised.
Using them after all, in my mind, is just bad science.
If so, try preparing it in a sterile manner. If you got the polysaccharide from sigma then it comes sterile. Open it in a laminar flow and use sterile water do dissolve it. The just add it into the DMEM.
Second, you can substitute speed and duration. i.e. if your centrifuge can't get up to the right speed you can increase the duration in the same proportion.
In the middle you can see two carriers and their sick sun.
On the bottom, because of the mutation (D) you get another band.
Make up a stock solution of 10 fold of the missing components and add it to the reaction buffer.
For woody parts the kits don't work manly because thy are rich with lignin and suberin.
The cloudiness you mention probably is one of them becoming unsalable and that's why they clog your filters.
I'm sad to say that most probably you will have to use a CTAB protocol preferably with PVP.
I cited an article and then asked if you did something different.
If nothing is different then your PI probably doesnt want to spend time and money on it.
If there is a novel thing then try running it again by your PI with a better pitch.
The main "faster" effect is because of the different Taq and buffer, is not shorter PCR cycle (10-15 cycle) that many had thought (even my PI). That's the reason I don't understand why she couldn't take the idea...
I return to my original question:
What is new in this multiplex pcr that hasn't been dun before?
If it's only the speed of the reaction then why should she spend time and money on it?
I think that the better science is to evaluate them separately (3'UTR for instance) and then sum them up.
I recently came across this article that did the same sort of thing.
Doing the same just faster but less accurate is bad from the clinical side. Say you give a wrong answer to someone in sepsis, you can kill him. Better wait a bit more, and get a better answer.
For cDNA -20șC.
For live cells use 10% DMSO, 10-90% FCS (depending on the protocol) and freeze in -70șC for up to a month or liquid N2 for longer.