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Minnie Mouse's Content

There have been 136 items by Minnie Mouse (Search limited from 19-November 18)



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#33702 graduate school

Posted by Minnie Mouse on 19 August 2009 - 06:28 PM in Career Advice

Some PI is willing to pay you the scholarship and pay for the full cost of the PhD training.

You may ask the aussie PI about it.



#31647 Happy Birthday Mdfenko

Posted by Minnie Mouse on 04 August 2009 - 03:51 AM in Chit Chat

Happy belated Birthday. :(



#31646 Scientific words

Posted by Minnie Mouse on 04 August 2009 - 03:49 AM in Scientific English

Would someone tell me which words are correct with hyphen, please? :(

overexpression or over-expression
upregulation or up-regulation
antitumor or anti-tumor
costimulatory or co-stimulatory
immunohistochemistry or immuno-histochemistry
downregulation or down-regulation
proapoptotic or pro-apoptotic
antiapoptotic or anti-apoptotic
prosurvival or pro-survival
chemoresistance or chemo-resistance
protooncogene or proto-oncogene


Thanks in advance.



#31119 Beginner Question

Posted by Minnie Mouse on 30 July 2009 - 04:39 PM in SDS-PAGE and Western Blotting

May I ask what protein expression are you looking for?



#28608 Yellow Laemmli sample buffer

Posted by Minnie Mouse on 07 July 2009 - 10:26 PM in SDS-PAGE and Western Blotting

You may wish to check the pH of the 2M Tris-HCl stock solution.

Hope this may help.



#28461 It's TJ's B-day

Posted by Minnie Mouse on 06 July 2009 - 07:09 PM in Chit Chat

Happy Birthday, toejam :)

Hope you receive many presents.



#27241 Research Assistant positions for semi fresh graduates like me

Posted by Minnie Mouse on 21 June 2009 - 08:12 PM in Venting and Counseling

Have you think about working overseas?



#27240 PhD problems

Posted by Minnie Mouse on 21 June 2009 - 08:10 PM in Life as a PhD Student and Postdoc

Can you manage to use your result data to write up a PhD thesis?



#27239 Re-using Culture flasks

Posted by Minnie Mouse on 21 June 2009 - 08:00 PM in Tissue and Cell Culture

are flasks THAT expensive you would mess up your results - just to recycle them? (taking into account the price of everthing else used leading to that point)

d



Dom,

Wonderful to have you back and talking common sense....your not my long lost brother?

Uncle Rhombus


Well, that all depends on where your funding comes from. I have to beg, borrow and steal just about everything because of a few expensive purchases in the beginning.
But yea, sure they are not THAT expensive to risk messing my results but I didnt know that until now.


I got the same problem during my PhD because I didn't have a sponsor to pay for my expensive PhD training cost :o .
I had to re-use the flasks for 5-10 times...not a good idea...but I did not have enough money to do experiments.

I know Uncle Rhombus will laugh at me. :(



#27237 Inconsistent ELISA results

Posted by Minnie Mouse on 21 June 2009 - 07:45 PM in ELISA and Immunoassay

you may try to

1. Calibrate the ELISA plate reader

2. use a very good multichannel pipette

I hope this may help.



#27236 Where might they be?

Posted by Minnie Mouse on 21 June 2009 - 07:37 PM in Chit Chat

They may be on holiday together. :o



#24503 Who is back?

Posted by Minnie Mouse on 16 May 2009 - 06:51 PM in Chit Chat

Uncle Rhombus is back :D

Here are his recent posts.
http://www.protocol-...sult_type=posts

I miss his brain :P - 30 years of tissue culture experience. :(



Dear Minnie Mouse,

Uncle Rhombus is back at work after a couple of months off. I missed you all as well

Kindest regards

Rhombus


Welcome back, Uncle Rhombus :D



#24074 Who is back?

Posted by Minnie Mouse on 11 May 2009 - 07:37 PM in Chit Chat

Welcome back, spanishflower. <_<



#23905 eHandbook of Receptor Classification and Signal Transduction

Posted by Minnie Mouse on 09 May 2009 - 09:12 PM in Free Stuff

http://www.sigmaaldr.../ehandbook.html



#23904 Convert g into rpm

Posted by Minnie Mouse on 09 May 2009 - 09:06 PM in General Lab Techniques

http://www.beckmanco...s/rotorcalc.asp



#23503 Silly things that make Scientists/Researchers happy

Posted by Minnie Mouse on 05 May 2009 - 02:11 PM in Chit Chat

printing double sided. makes me feel "environmentally friendly" :D


same here :wacko:



#23064 Silly things that make Scientists/Researchers happy

Posted by Minnie Mouse on 29 April 2009 - 02:01 PM in Chit Chat

playing with the bubble wrap. POP POP POP ;)

It makes me happy.



#22292 male/female ratio

Posted by Minnie Mouse on 20 April 2009 - 02:24 PM in Chit Chat

i'd say for the quality of their research rather than their gender.


Does this mean people often look at gender before looking at the quality of work? :) :ph34r:

If I am Mickey, people would respect me more in research.

I remembered reading from Science or Nature that a researcher converted from female to male, and noticed the difference in people treating her and him.



#22157 Waterless Water Baths?

Posted by Minnie Mouse on 17 April 2009 - 04:52 PM in Cell Biology Products

We use a "sand bath", an old, leaking water bath with clean, sterilised quartz sand. Not bad for some experiments.
And they are also offered new by some companies . I guess sand has better heat transmission compared to beads.



The classic sand bath has some similar qualities to the bead bath, however, the Bath Beads by LabArmor have a superior rate of high efficiency thermal transfer when compared to sand, plus instrument burnout protection, and are mobile and field use compatible.

DW


Have you done a test to compare the bead and the sand bath?



#22153 Freedom Rocker - Save yourself from babysitting your gels!

Posted by Minnie Mouse on 17 April 2009 - 03:56 PM in Lab Equipments

Is this machine durable?

I am very interest in this machine, but worry that it may breakdown after two years (after the 2-year warranty).



#21934 different staining intensity

Posted by Minnie Mouse on 15 April 2009 - 08:17 PM in Flow Cytometry

Why is the staining intensity weaker in fixed permeabilized cells than non-fixed cells?

1. The permeabilized cancer cells were fixed with 3.7% paraformaldehyde and then permeabilized with saponin.
2. cells were stained with either A or B antibody
3. the secondary conjugate was anti-mouse FITC


The antigen was expressed on the cell surface.
Two monoclonal antibodies (A and B ) against the same antigen were used to stain the cells.
A and B antibodies stained weaker in fixed permeabilized cells than non-fixed cells.

Attached File  Minnie__s_flow_cytometry_result.ppt   56KB   590 downloads


Why?



Thanks in advance.



#21754 automated protein structure search

Posted by Minnie Mouse on 13 April 2009 - 09:54 PM in Bioinformatics and Biostatistics

BLAST

http://blast.ncbi.nl...h.gov/Blast.cgi



#21705 lab conspiracy

Posted by Minnie Mouse on 13 April 2009 - 02:57 PM in Venting and Counseling

may tell your PI that you may wish to do PhD in this lab in future.

then he may give you a project to do.



#21704 Reuse DNA spin column

Posted by Minnie Mouse on 13 April 2009 - 02:55 PM in Molecular Biology

I am confused about the method exactly.

It says to soak in 1 M HCl then rinse in sterile distilled water and equilibriate with Buffer QBT.

When they say rinse do they really mean just rinse or fill the column and keep cetrifuging the water through?

Also same thing about equilibrate. What does that mean exactly.


fill the column and centrifuge to get rid of the water



#21703 Tumor size statistics in 4 groups of transgenic mice!!

Posted by Minnie Mouse on 13 April 2009 - 02:51 PM in Bioinformatics and Biostatistics

1. Do I have to calculate average or mean?
2. What Test do I have to use to get p values?
3. When I draw the data above in Excel Bar graph, I want to present average/mean as well as 'deviate'. What deviate do I have to use? standard error(SE) OR standard deviation(SD)?

Thank you so much in advance!![/size]


1. average = mean

2. two-way ANOVA

3. SD

Hope this may help.




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