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Minnie Mouse's Content
There have been 136 items by Minnie Mouse (Search limited from 23-January 19)
overexpression or over-expression
upregulation or up-regulation
antitumor or anti-tumor
costimulatory or co-stimulatory
immunohistochemistry or immuno-histochemistry
downregulation or down-regulation
proapoptotic or pro-apoptotic
antiapoptotic or anti-apoptotic
prosurvival or pro-survival
chemoresistance or chemo-resistance
protooncogene or proto-oncogene
Thanks in advance.
are flasks THAT expensive you would mess up your results - just to recycle them? (taking into account the price of everthing else used leading to that point)
Wonderful to have you back and talking common sense....your not my long lost brother?
Well, that all depends on where your funding comes from. I have to beg, borrow and steal just about everything because of a few expensive purchases in the beginning.
But yea, sure they are not THAT expensive to risk messing my results but I didnt know that until now.
I got the same problem during my PhD because I didn't have a sponsor to pay for my expensive PhD training cost .
I had to re-use the flasks for 5-10 times...not a good idea...but I did not have enough money to do experiments.
I know Uncle Rhombus will laugh at me.
Uncle Rhombus is back
Here are his recent posts.
I miss his brain - 30 years of tissue culture experience.
Dear Minnie Mouse,
Uncle Rhombus is back at work after a couple of months off. I missed you all as well
Welcome back, Uncle Rhombus
i'd say for the quality of their research rather than their gender.
Does this mean people often look at gender before looking at the quality of work?
If I am Mickey, people would respect me more in research.
I remembered reading from Science or Nature that a researcher converted from female to male, and noticed the difference in people treating her and him.
We use a "sand bath", an old, leaking water bath with clean, sterilised quartz sand. Not bad for some experiments.
And they are also offered new by some companies . I guess sand has better heat transmission compared to beads.
The classic sand bath has some similar qualities to the bead bath, however, the Bath Beads by LabArmor have a superior rate of high efficiency thermal transfer when compared to sand, plus instrument burnout protection, and are mobile and field use compatible.
Have you done a test to compare the bead and the sand bath?
1. The permeabilized cancer cells were fixed with 3.7% paraformaldehyde and then permeabilized with saponin.
2. cells were stained with either A or B antibody
3. the secondary conjugate was anti-mouse FITC
The antigen was expressed on the cell surface.
Two monoclonal antibodies (A and B ) against the same antigen were used to stain the cells.
A and B antibodies stained weaker in fixed permeabilized cells than non-fixed cells.
Minnie__s_flow_cytometry_result.ppt 56KB 602 downloads
Thanks in advance.
I am confused about the method exactly.
It says to soak in 1 M HCl then rinse in sterile distilled water and equilibriate with Buffer QBT.
When they say rinse do they really mean just rinse or fill the column and keep cetrifuging the water through?
Also same thing about equilibrate. What does that mean exactly.
fill the column and centrifuge to get rid of the water
1. Do I have to calculate average or mean?
2. What Test do I have to use to get p values?
3. When I draw the data above in Excel Bar graph, I want to present average/mean as well as 'deviate'. What deviate do I have to use? standard error(SE) OR standard deviation(SD)?
Thank you so much in advance!![/size]
1. average = mean
2. two-way ANOVA
Hope this may help.