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#169555 Sonication of small PCR amplicon

Posted by ForEpi on 22 July 2014 - 11:16 PM in General Lab Techniques

Hi colleagues,

 

what happens to an amplicon of, say, 500bp after 1 round or 2 rounds of shearing? Will I get a majority of intact amplicons and an equal distribution of all other fragment sizes or will there be a tailing off with decreasing length? Anyone have any experience with this?

 

Best,

 

Bram





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