Good morning, dear all!
How can I easely recognize if my protein is expressed as dimer or as a monomeric protein?
I thik my protein tend ta associate in dimer in presence of palmitic acid acid pruduced by bacteria during expression step.
Good morning dear all, I want to espress a big protein..about 1070 aa.
I want to insert six His to this protein to improve the purification. are suffient 6 His or the protein is to big to purify with a Ni column?
Thank You, I'm happy for Your e-mail. as i've said You before, when I trasform my competents I obtain few, few colonies. So it's important for me to learn a new method to prepare competents. waiting Your e-mail (and your protocols), Regards,
How many samples do you need? I can offer you one protocoll for about 60 and one for about 250.... Greets