Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

FRAROAR's Content

There have been 11 items by FRAROAR (Search limited from 02-April 19)


Sort by                Order  

#50798 BEAGLE, WHY?

Posted by FRAROAR on 11 December 2009 - 03:37 AM in Animal and Zoology

good morning.
Why beagles are so important for drug discovery? why this dog species and not an other?
Thanks



#50792 Beagle model

Posted by FRAROAR on 11 December 2009 - 01:13 AM in Pharmacology and Pharmaceutics

good morning.
Why beagles are so important for drug discovery? why this dog species and not an other?
Thanks



#47541 Dimer or monomer

Posted by FRAROAR on 25 November 2009 - 07:25 AM in SDS-PAGE and Western Blotting

Good morning, dear all!
How can I easely recognize if my protein is expressed as dimer or as a monomeric protein?
I thik my protein tend ta associate in dimer in presence of palmitic acid acid pruduced by bacteria during expression step.

reagards,thank You very much for the help

fra



#39971 smallest nucleotidic insertion

Posted by FRAROAR on 15 October 2009 - 06:45 AM in Molecular Biology

Good morning. whisch is the smallest insert to use in a ligation reaction( plasmid length about 7500 nucleotides, insert length about 75 mucleotides)
Thank You very much



#39212 t7 promoter

Posted by FRAROAR on 08 October 2009 - 04:52 AM in Molecular Cloning

HI dear all!
The T7 promoter is present inside the vector pCW?
is possible to express a protein using pCW vector with BL21 cells?
Thanks



#39210 t7 promoter......

Posted by FRAROAR on 08 October 2009 - 04:28 AM in Molecular Biology

HI dear all!
The T7 promoter is present inside the vector pCW?
is possible to express a protein using pCW vector with BL21 cells?
Thanks



#24559 His Tag protein

Posted by FRAROAR on 18 May 2009 - 03:54 AM in Molecular Cloning

Good morning dear all, I want to espress a big protein..about 1070 aa.
I want to insert six His to this protein to improve the purification. are suffient 6 His or the protein is to big to purify with a Ni column?

Thanks,

Fra



#18963 Competent DH5 alpha

Posted by FRAROAR on 16 March 2009 - 07:12 AM in Molecular Cloning

Thank You, I'm happy for Your e-mail. as i've said You before, when I trasform my competents I obtain few, few colonies. So it's important for me to learn a new method to prepare competents.
waiting Your e-mail (and your protocols),
Regards,


Francesco



How many samples do you need? I can offer you one protocoll for about 60 and one for about 250.... Greets




#18940 Competent DH5 alpha

Posted by FRAROAR on 16 March 2009 - 03:31 AM in Molecular Cloning

Hi, I'm searching a good protocol to have wonderful competent cells..
When I do transformation with my competent cells I don't have so much colonies.
Who can help me?



#13726 kpi ?

Posted by FRAROAR on 29 January 2009 - 05:06 AM in Molecular Cloning

Hi, i must prepare 50 mM Kpi pH 7,4. How? what I must weigth and how to reach the rigth pH?
Thanks



#13336 cDNA to find

Posted by FRAROAR on 26 January 2009 - 05:35 AM in Molecular Cloning

Hi dear all! If I need dog cDNA to clone a gene which company has it?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.