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Tysia91's Content

There have been 2 items by Tysia91 (Search limited from 26-January 19)


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#180856 LiAc transformation - HELP!

Posted by Tysia91 on 29 October 2018 - 01:53 AM in Microbiology

Dear Bob1,

 

Thank you for your help, I'm very grateful.

I used this method and didn't work with my plasmid. I realy want to find method from Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor NY (1994, Kaiser et, al.) becouse I want know what they did diffrent to achieve the success.

 

Best reagrds,

Justyna




#180854 LiAc transformation - HELP!

Posted by Tysia91 on 26 October 2018 - 11:41 AM in Microbiology

Hi guys, 

 

if anybody has a copy of LiAc transofrmation' protocol from Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor NY (1994, Kaiser et, al.).

 

 

I have a problem with transformation - I received a plasmid  which overproduce SLA1. I want to transform strains which have deleted genes involved in assembly in the cytoskeleton. In the paper is written that they used transformation protocol from that book but unfortunately I can't find it anywhere .

 

I tried many protocols but none work. I really want to test protocol from that book. 

 

Please, help me! You are my last hope.

 

Thank you,

Justyna





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