Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

hops proteon's Content

There have been 3 items by hops proteon (Search limited from 16-September 18)


Sort by                Order  

#180741 Protein expression issue

Posted by hops proteon on 11 September 2018 - 11:44 PM in Protein Expression and Purification

I agree with Thelymitra pulchella. Your protein may be aggregating into inclusion body form.  For avoiding this situation, you may need some advises, such as 1) fusion of the target protein with a more soluble partner, typically a bacterial protein; 2) co-expression of folding catalysts and chaperones; 3) expression under culture conditions which reduce the translation rates or affect the intracellular environment; and 4) modification of the protein sequence.




#180383 Percentage of Outer Membrane Protein

Posted by hops proteon on 04 June 2018 - 10:48 PM in Protein and Proteomics

Pichia cell protein expression purification

Tag purification: His tag, affinity purified;

Ion exchange, gel filtration

It’s recommended you download this GE protein purification manual(sample preparation, protein purification, electrophoresis, blotting, and cell culture.) (https://www.gelifesc...m/en/us/support)




#180376 GLUT1 and GLUT4 expression in serum

Posted by hops proteon on 01 June 2018 - 12:09 AM in Protein Expression and Purification

Do you want to get this protein?





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.