Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

hudgie's Content

There have been 1 items by hudgie (Search limited from 20-September 18)


Sort by                Order  

#180324 Northern blot with a RNA probe labeled with a fluorophore

Posted by hudgie on 11 May 2018 - 07:12 AM in Molecular Biology

I have some fluorophore labeled RNA probes that I want to try in a Northern Blot analysis using Qiagen RNeasy kit purified RNA.

I want to image the blot on the LiCor Odyssey imager/scanner. They recommend using a Northern Max kit with DIG labeling of the RNA probe prep by RT-PCR but I already have fluorescent labeled custom probes that I don't want to waste.

Every northern blot protocol I see for RNA is for DIG-labeled probes. Does any have any "protocol suggestions" for me to use these expensively made custom fluorophore RNA probes??





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.