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hudgie's Content

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#180324 Northern blot with a RNA probe labeled with a fluorophore

Posted by hudgie on 11 May 2018 - 07:12 AM in Molecular Biology

I have some fluorophore labeled RNA probes that I want to try in a Northern Blot analysis using Qiagen RNeasy kit purified RNA.

I want to image the blot on the LiCor Odyssey imager/scanner. They recommend using a Northern Max kit with DIG labeling of the RNA probe prep by RT-PCR but I already have fluorescent labeled custom probes that I don't want to waste.

Every northern blot protocol I see for RNA is for DIG-labeled probes. Does any have any "protocol suggestions" for me to use these expensively made custom fluorophore RNA probes??

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