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Oracle602's Content

There have been 3 items by Oracle602 (Search limited from 12-August 19)

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#9585 Transformation Efficiency

Posted by Oracle602 on 26 December 2004 - 10:17 AM in Molecular Biology

Trying to incorporate various environmental resistance genes

#9580 I'm lost... What to do after PhD???

Posted by Oracle602 on 25 December 2004 - 07:08 PM in Career Advice

In my personal opinion, whatever you do, don't give up! Finish the job, do the research. If I were to drop what you are doing I would feel that those years of life would be a waste. When you're done, you can use your knowledge to incorporate things you like with the field you specialize in. (Doctor hating medicine and loving computers made a clinical application program that all doctors use).
But then again, I"m not speaking from experience. Hopefully some members of this board can give their experienced opinion.

Good luck to you!


#9579 Transformation Efficiency

Posted by Oracle602 on 25 December 2004 - 06:59 PM in Molecular Biology

What factors would you say could affect the transformation efficiency in incorporating supercoiled plasmid DNA into a unicellular eukaryote?
I used the calcium chloride protocol. Temperature of incubation? heat shock? amounts of cells/plasmid used?

Thanks in Advance,

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