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Eaglefan's Content

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#177669 [Ca2+] in a muscle lysate

Posted by Eaglefan on 13 February 2016 - 08:23 PM in Cell Biology

Hello,

 

We would normally use single muscle fibers to determine intracellular calcium, but the only sample we have is pre-homogenized lysate (RIPA buffer). Is there any method that we could use as a proxy for intracellular calcium levels?

 

Any help would be greatly appreciated!
 





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