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Fabian Yu's Content

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#172807 Question: Can I cut out rb glob PA Terminator

Posted by Fabian Yu on 21 January 2015 - 09:11 AM in Molecular Cloning

Hi There,


I am doing a cloning project with the pCAGGs promoter.  I already ligated a fragment of my gene of interest  in the plasmid, and now I need to add an IRES::Puro sequence.  So far my ligation is not working but I'm not sure whether it is b/c I cut out the rb glob PA Terminator in the plasmid.  I know the poly a site helps with transcription but is it essential for my plasmid? Only reason I did that was due to the cut site  issue 





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