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Hi There,
I am doing a cloning project with the pCAGGs promoter. I already ligated a fragment of my gene of interest in the plasmid, and now I need to add an IRES::Puro sequence. So far my ligation is not working but I'm not sure whether it is b/c I cut out the rb glob PA Terminator in the plasmid. I know the poly a site helps with transcription but is it essential for my plasmid? Only reason I did that was due to the cut site issue
thank
