Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

uginong's Content

There have been 1 items by uginong (Search limited from 19-September 19)

Sort by                Order  

#6678 SDS-PAGE: casting

Posted by uginong on 19 August 2004 - 09:22 PM in SDS-PAGE and Western Blotting


i know this is going against popular opinion but try running ur gel at a fixed current instead of voltage. a good try would be 15 mA to 20 mA depending on the size of gel. if u are able to run it at cold temperature, i.e. cold running buffer in a cold room or fridge.

for my case this greatly increased band intensity, especially the smaller ones that migrates close to the dye which usually diffuses.

there are logical reasons for this, i think u can look it up in textbooks.

hope that helped.


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.