When I was designing primers using PRIMER3
The screen outputs like follows
Forward primer ACTGACTG
Reverse primer CTGACTGA
I was wondering do I need to reverse and complement my reverse primer when ordering my primers?
For example, I should order TCAGTCAG for my real reverse primer
Thank you.
Hi,
Yes, it is possible that the primers may not have been designed accurately. I would suggest you to evaluate your primers using NetPrimer, which is freely accessible.
Here is the link to NetPrimer:
(http://www.premierbi...rimer/index.htm)
Also, I found a great resource that claims to be exclusively designed for bacterial identification:
(http://www.premierbi...ation/realtime-PCR/species-identification.html)
Hope this helps.
Sabastian