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flashboy's Content

There have been 3 items by flashboy (Search limited from 19-September 19)


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#16752 starting total RNA concentration for miRNA analysis

Posted by flashboy on 24 February 2009 - 05:13 AM in PCR, RT-PCR and Real-Time PCR

Hi all,

If i wanted to use total RNA samples for miRNA analysis, what starting concentration should I use? the Ambion kit has a heart total RNApositive control at 25ng/ul.... Would it be bad to use a higher concentration than this?

Cheers



#16286 SYBR Green miRNA analysis

Posted by flashboy on 20 February 2009 - 02:39 AM in PCR, RT-PCR and Real-Time PCR

Hi all,

just starting some miRNA analysis using SYBR green and ambion primer kits... just a quick query... can i use any sybr green real-time pcr mastermix? the one i currently use for my mRNA real-time (from primerdesign) is so cost effective i don't want to have to go out and buy another..... any ideas?



#16285 small RNAs extraction

Posted by flashboy on 20 February 2009 - 02:35 AM in Molecular Biology

i've just used the ambion kit to isolate small RNA's from human adipose tissue... using small chunks (i guess about 20-50mg) of tissue i averaged about 10-20ng/ul of RNA (nanodrop)




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