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#121485 Quantifying DNA from Buccal Swabs

Posted by ca.dreaming on 13 October 2011 - 04:57 PM in Molecular Biology

So I'm collecting DNA from Buccal Swabs but having some trouble quantifying it. Is there any DNA extraction kit that allows the use of Nanodrop???
Otherwise, Im trying to create a standard curve for fluorescence using Hoechst Dye. Right now I've got DNA concentrations between 0 and 2.6 micrograms/ml and 1 mg/ml Hoechst Dye in a 100microliter well with buffer.
352nm excitation, 461nm emission wavelengths...

But my values are all over the board and not linear at all.
Any idea where I could be going wrong?

Any help would be much appreciated!
Best,
Constance




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