Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

ca.dreaming's Content

There have been 1 items by ca.dreaming (Search limited from 15-April 20)

Sort by                Order  

#121485 Quantifying DNA from Buccal Swabs

Posted by ca.dreaming on 13 October 2011 - 04:57 PM in Molecular Biology

So I'm collecting DNA from Buccal Swabs but having some trouble quantifying it. Is there any DNA extraction kit that allows the use of Nanodrop???
Otherwise, Im trying to create a standard curve for fluorescence using Hoechst Dye. Right now I've got DNA concentrations between 0 and 2.6 micrograms/ml and 1 mg/ml Hoechst Dye in a 100microliter well with buffer.
352nm excitation, 461nm emission wavelengths...

But my values are all over the board and not linear at all.
Any idea where I could be going wrong?

Any help would be much appreciated!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.