Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

raa's Content

There have been 1 items by raa (Search limited from 21-September 19)


Sort by                Order  

#105701 ATP and glucose in the external solution for patch clamping

Posted by raa on 04 April 2011 - 07:23 AM in Neuroscience

Hi,

I am a Post-Doc at Cornell University and would like some advice regarding the external solution used to patch clamp rat cortical neurons. I am isolating embrionic cells and have seen in the literature that I need to add ATP and glucose to the external solution. I have also heard that I need to prepare the ATP on itself, separate in aliquots, freeze and use it on the day of the experiment. Aparently, glucose should be weighted and added to the external solution on the day of the experiment. Are all these information right? Is there any other detail that I should pay attention about ATP and glucose?

All help would be really appreciated!

Thank you!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.