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Obayed's Content

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#100638 Purification of His tagged protein

Posted by Obayed on 14 February 2011 - 11:29 PM in Protein and Proteomics


I am purify a his tagged protein expressed in E coli Bl 21DE3. But after gel filtration there are still contamination of different size of protein. I tried Ion exchange chromatography but it did not worked. Is there any thing i can do so that the unexpected protein will not be expressed or how to purify that one


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