Currently I'm working on GH3 cell lines. But contamination always happen. When I check the media, the media is okay.
When I'm doin the subculturing, I always make sure I'm doing it aseptically. Lets say if I'm not sure whether the pippete had touch something in the laminar flow/clean bench, I will change to a new one.
I did notice this GH3 cell very easy to get contaminated compared to HepG2 cells.