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rpjkmust916's Content

There have been 4 items by rpjkmust916 (Search limited from 17-February 19)


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#154430 PI stain for cell viability~~Compensation? Suggestion needed~~^^

Posted by rpjkmust916 on 30 April 2013 - 07:18 AM in Flow Cytometry

Thanks~~~Posted Image



#135704 GH3 cells

Posted by rpjkmust916 on 09 June 2012 - 10:32 PM in Tissue and Cell Culture

Thanks Kat T~~Posted Image

This is very informative and I will try~~Posted Image



#134380 GH3 cells

Posted by rpjkmust916 on 10 May 2012 - 10:34 PM in Tissue and Cell Culture

Thanks Kat T`~~~

I never check the trypsin. And for PBS, I'm really sure its not contaminated cos I use the same PBS to subculture HepG2.

I put antibiotic in my media but i never check the low level contamination of my stock cells, may you explain how to do this?

Thanks again~~Posted Image



#134301 GH3 cells

Posted by rpjkmust916 on 10 May 2012 - 12:39 AM in Tissue and Cell Culture

Hi friends~~~

Currently I'm working on GH3 cell lines. But contamination always happen. When I check the media, the media is okay.
When I'm doin the subculturing, I always make sure I'm doing it aseptically. Lets say if I'm not sure whether the pippete had touch something in the laminar flow/clean bench, I will change to a new one.


I did notice this GH3 cell very easy to get contaminated compared to HepG2 cells.


Please give some advice...



Thank you in advance.


-rpjkmust916-




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