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zingmatter's Content

There have been 3 items by zingmatter (Search limited from 07-March 20)

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#6221 phosphatase inhibitors

Posted by zingmatter on 25 July 2004 - 11:51 PM in Protein and Proteomics

I want to do phospho-IP from mammalian cell extracts and am trying to select phospahtase inhibitors while avoiding anything too toxic (if possible).

Any have any ideas or experience with this?


#5488 Quantum dots in FACS

Posted by zingmatter on 29 April 2004 - 08:13 AM in Cell Biology

Has anyone used Quantum Dots (avidin conjugated, 585nm) for FACS analysis?



#4864 Stable cell lines using Flp-In

Posted by zingmatter on 08 January 2004 - 06:28 AM in Cell Biology


I want to create a cDNA library that is stably transfected into HEK 293 cells using Invitrogen's Flp-In kit.

My problem is that I need to know what percentage of integration I can see (using the pre-made FRT carrying cells). If the percenatage of cells that integrate DNA is not good then I'd be struggling to get a full representation of the cDNA library.

My alternative is to use a retroviral method but I don't want to go down that route if I can avoid it.

If anyone has any experience with Flp technology I appreciate some feed back.



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