Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

yanks1ny's Content

There have been 1 items by yanks1ny (Search limited from 22-January 19)


Sort by                Order  

#164414 Ligand-controllable degron (Shield-1) for tunable protein stability

Posted by yanks1ny on 22 January 2014 - 10:32 AM in Molecular Biology

I was wondering if anyone here has done any work utilizing Shield-1 technology (ligand-controllable degron for rapidly regulation levels of exogenous protein). It's quite a fascinating system (no Shield-1 ligand --> degron-fused protein is degraded vs. add Shield-1 ligand --> degron-fused protein is stabilized). We have had some success so far, where we can transfect cells with our degron-tagged constructs and see a wonderful time-dependent stabilization upon the addition of the Shield-1 ligand.

 

However, we noticed that baseline levels (in cells transfected with our degron-construct, but not treated with Shield-1) is not as low as I had originally hoped (although we see a clear increase in protein levels upon the addition of Shield-1). Obviously there will be variability based upon cell type being used and the protein being studied, but does anyone know if this is a common problem? If so, has anyone heard of any suggestions or any possible workaround solutions for reducing the protein level (or is this just a caveat of the system in general)?

Attached Thumbnails

  • Shield example.png




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.