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vgupta's Content

There have been 6 items by vgupta (Search limited from 24-August 18)


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#8439 aminoethyl dextran

Posted by vgupta on 04 November 2004 - 11:11 AM in Immunology Products

from where can i buy aminoethyl dextran or AED?



#8438 aminoethyl dextran

Posted by vgupta on 04 November 2004 - 11:10 AM in Cell Biology Products

from where can i buy aminoethyl dextran or AED?



#6507 single cell PCR

Posted by vgupta on 11 August 2004 - 11:38 AM in Molecular Biology

Has anyone tried single cell PCR? Have you faced any problems with it?
Would anyone have a protocol for it and some trouble shooting tips?



#5978 PCR contamination in negative control

Posted by vgupta on 01 July 2004 - 06:57 AM in Molecular Biology

:( Thanks for your suggestions. Do you think primer purification or mycoplasma contamination could be a problem even if the band is of the same size as the product expected in positive control (with DNA)? I am trying to amplify a prokaryotic vector sequence integrated into mouse genome. My primers are from qiagen. One particular set of primers works well with this construct.



#5972 PCR contamination in negative control

Posted by vgupta on 30 June 2004 - 12:30 PM in Molecular Biology

:( I have been contamination problems in my no DNA control of PCR. I have changed all the solutions and have got new primers made. But some how this contaminating band always appears. This happens with a particular DNA clone and not with other clones. Also there is one particular seuence of primers which doesn't give me contamination with this clone. I set up the reactions in a hood. I don't know what to do?



#5971 RNA isolation from mouse blood

Posted by vgupta on 30 June 2004 - 12:25 PM in Molecular Biology

Does anyone know which kit can be used for isolation of RNA from mouse blood. What is the yield from 200 ul of blood? Also, what is the mouse blood cell count, I mean the total WBCs and RBCs?
Thanks.




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