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Sa-Fa's Content

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#64725 antibody expression vector ??

Posted by Sa-Fa on 30 March 2010 - 12:39 AM in Molecular Cloning

Hi!
I am working on the cloning of a recombinant antibody (heavy and light chain) and i am looking for a vector to express this antibody in mammalian cells. I would like to know what would be the best vector to express both chains at high levels in mammalian cells: a bicistronic? a bidirectional? or both chain in separate vectors? is there any commercial vector for rec Antibody expression vectors?

Thanks for all helps!
Sa Fa




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