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s2paik's Content

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#135732 virus transduction vs plasmid transfection

Posted by s2paik on 10 June 2012 - 05:58 PM in Molecular Biology

Recently I have transducted/transfected cells with a GFP tagged-protein, and observed that there were some noticeable difference
in protein expression (by fluorescence visualization) between the lentivirus transducted and plasmid transfected cells.
The sequence of plasmid used for virus transduction and transfection have been validated using sequencing. Same cell line was used.

Is this difference in expression between virus transducted and plasmid transfected cells expected?
If not, have anyone had any experience with such results, or have any suggestions/comments for this?

Theoretically, shouldn't the protein expression be the same?

Any suggestion, comments are appreciated!!

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