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christy's Content

There have been 2 items by christy (Search limited from 03-June 19)

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#174097 When is safe to stop

Posted by christy on 01 April 2015 - 07:25 PM in Molecular Biology



After PCR, we can keep samples at 4 C or purifiy the PCR products and store at - 20 C for long time. After ligation,  transform next day (if you ligate at 4 C).

#173668 Troubleshooting with cloning

Posted by christy on 04 March 2015 - 04:06 PM in Molecular Biology



Try this link for ligation calculation http://www.insilico..../Lig_Input.html


what kind of ligation it is, sticky or blunt? Did you do self ligation. How long you are doing ligation and what temperature you are incubating. After ligation, did you screen your clones before sequencing.



All the best !!!

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