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vahid's Content

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#80736 addition of BclI restriction site to PCR primer

Posted by vahid on 23 July 2010 - 08:07 AM in Molecular Biology

Hi
are you sure your BclI enzyme can cut the site when you did not used any sequence before the restriction site?
I do not know Why new england biolab did not defined any sequence for this enzyme. I have to design a primer with the site for this enzyme but I am not sure it can cut the sequence <_<




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