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Minnie Mouse's Content

There have been 136 items by Minnie Mouse (Search limited from 22-January 19)



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#17756 A manual of techniques for separation, detection and recovery of nucleic acids a

Posted by Minnie Mouse on 04 March 2009 - 02:04 PM in Free Stuff

Thank you lactolee



#13838 all gone??????

Posted by Minnie Mouse on 29 January 2009 - 05:59 PM in Chit Chat

research gate is over, but has anybody visited this molecular station site???? It seems there are some vultures around....... :wacko:

do U remember there was another dude asking for name for a scientist facebook. I wonder what happened to him.


But some are lost, for example toejam, vetticus3, fred33, aimikins (?). Have a poor memory for (nick-)names.


Rhombus, Calvin*, cellcounter, and phage434 may be lost.



#14138 Apoptosis Protocol

Posted by Minnie Mouse on 01 February 2009 - 02:36 PM in Apoptosis, Necrosis and Autophagy

Introduction to apoptosis
http://www.abcam.com...g...e&rid=11367

Annexin V detection protocol
http://www.abcam.com...g...e&rid=11369

Apoptosis induction protocol
http://www.abcam.com...g...e&rid=11368

Caspase detection protocol
http://www.abcam.com...g...e&rid=11370

DNA fragmentation analysis protocol
http://www.abcam.com...g...e&rid=11371



#21754 automated protein structure search

Posted by Minnie Mouse on 13 April 2009 - 09:54 PM in Bioinformatics and Biostatistics

BLAST

http://blast.ncbi.nl...h.gov/Blast.cgi



#20615 B*%$%y government! Nanny state gone too far

Posted by Minnie Mouse on 31 March 2009 - 03:21 PM in Venting and Counseling

or develop a nut vaccine



#31119 Beginner Question

Posted by Minnie Mouse on 30 July 2009 - 04:39 PM in SDS-PAGE and Western Blotting

May I ask what protein expression are you looking for?



#14265 Best storage temperature for samples - serum, protein, tissue, DNA, RNA

Posted by Minnie Mouse on 02 February 2009 - 08:20 PM in General Lab Techniques

Due to spatial limitations in our lab freezers i want to reorganize my samples. can anyone of you tell me where different kinds of samples should be longtime stored?

-20°C, -80°C, -196°C...

Serum
Tissue
Proteinextracts
RNA
DNA
Bacterial Strains
Tissue Sections on Slides
...

Thanks for recommendations!

-moljul-
________________________________________________________________________________
_____________

Why don't you just keep them at the same temps. where they are stored right now. Even if you reorganize you shouldn't put them into different freezers. Sounds like an exam question to me ;-)


I help you anyway:
- DNA: Plasmids at -20C, Genomic at 4C. DNA can fall out of solution if stored at -20C. With plasmids that is no problem because you can vortex them and get them back into solution. Genomic DNA is dangerous to vortex because you might break the DNA
- RNA: Is less stable than DNA so we store it at -80. But you should be able to store it at -20C too
- Bacteria: -80C
- Serum: -20C
- Protein: - 20C
- Tissue: Depends. Ours are formalin fixed so we leave them in the fridge. Flash frozen samples in liquid nitrogen

-UGA80-
________________________________________________________________________________
_____________

-4`C -- Tissue section on slides
-20`C -- Serum, DNA, RNA, protein extracts
-80`C -- Bacterial strains (in LB),
-196`C -- Tissue

-tzyyyue-



#14268 BioForum Archives (1999-2009) are available for browsing and searching

Posted by Minnie Mouse on 02 February 2009 - 08:29 PM in News and Announcements

Thank you.

Luckily, we haven't lost everything. :P



#14503 BioForum trawling

Posted by Minnie Mouse on 04 February 2009 - 01:00 PM in Chit Chat

There is a "Search" buttom at the top right hand corner and a "Forum Archives" buttom at the top...slightly to the right.

Hope this may help.



#14638 Buffers that are good for cell culture.

Posted by Minnie Mouse on 05 February 2009 - 01:33 PM in Tissue and Cell Culture

What cell line are you growing?



#14662 Cell count using a hemocytometer

Posted by Minnie Mouse on 05 February 2009 - 03:28 PM in Tissue and Cell Culture

Cell count using a hemocytometer

http://www.abcam.com...g...e&rid=11454



#13855 CHEMICAL ILLUSIONS

Posted by Minnie Mouse on 29 January 2009 - 08:09 PM in Chit Chat

I think I may close this thread.



#13816 Concepts and Application of Inferential Statistics

Posted by Minnie Mouse on 29 January 2009 - 02:09 PM in Bioinformatics and Biostatistics

Concepts and Application of Inferential Statistics


http://faculty.vassa...ry/webtext.html



#21460 confusion about 5'UTR

Posted by Minnie Mouse on 09 April 2009 - 09:44 PM in Molecular Cloning

Hi Chicken,

Easter is coming.
Some people are on holiday or are busy finishing the experiments before the holiday.

After Easter Holiday, there will be more people to answer the posts.



#23904 Convert g into rpm

Posted by Minnie Mouse on 09 May 2009 - 09:06 PM in General Lab Techniques

http://www.beckmanco...s/rotorcalc.asp



#13515 Current Protocol by John Wiley & Son

Posted by Minnie Mouse on 27 January 2009 - 01:34 PM in Tissue and Cell Culture

Current Protocol by John Wiley & Son

Please check link.
https://commerce.inv...44C7DCE18F84CBD



#13523 Current Protocol by John Wiley & Son

Posted by Minnie Mouse on 27 January 2009 - 01:46 PM in Protein and Proteomics

Current Protocol by John Wiley & Son

https://commerce.inv...070AFD1A10EB30C



#21934 different staining intensity

Posted by Minnie Mouse on 15 April 2009 - 08:17 PM in Flow Cytometry

Why is the staining intensity weaker in fixed permeabilized cells than non-fixed cells?

1. The permeabilized cancer cells were fixed with 3.7% paraformaldehyde and then permeabilized with saponin.
2. cells were stained with either A or B antibody
3. the secondary conjugate was anti-mouse FITC


The antigen was expressed on the cell surface.
Two monoclonal antibodies (A and B ) against the same antigen were used to stain the cells.
A and B antibodies stained weaker in fixed permeabilized cells than non-fixed cells.

Attached File  Minnie__s_flow_cytometry_result.ppt   56KB   602 downloads


Why?



Thanks in advance.



#16918 dilution question help

Posted by Minnie Mouse on 25 February 2009 - 01:44 PM in General Lab Techniques

Stock A: 200ng/ul

need 10ug in 200ul, therefore final concentration is 10ug/200ul

CV=CV

stock concentration x required stock volume = final concentration x final volume
200ng/ul x V = 10ug/200ul x 200ul
V = 10ug / 200ng/ul
V = 10ug / 0.2ug/ul
V= 50ul

Therefore the required stock volume is 50ul + 150ul buffer/water



Stock B: 350ng/ul

stock concentration x required stock volume = final concentration x final volume
350ng/ul x V = 10ug/200ul x 200ul
V = 10ug / 350ng/ul
V = 10ug / 0.35ug/ul
V= 28.6ul

Therefore the required stock volume is 28.6ul + 171.4ul buffer/water


Hope this may help.



#16947 dilution question help

Posted by Minnie Mouse on 25 February 2009 - 07:32 PM in General Lab Techniques

Thanks for the formula.

Say I have DNA that measured 1 mg/ml concentration and my target concentration is 100ug, 50ul final, how would I do this? I tried using it with the formula below, but it doesn't work very well. How can I get 100ug of 1 mg/ml somehow?

1mg/ml = 1ug/ul

(1ug/ul)*V = (100ug/50ul)* 50ul
(1ug/ul)*V=(2ug/ul)*50ul
V = 100ug/(1ug/ul)
V = 100ul


The final concentration is 2ug/ul which is 2mg/ml... twice as much as the stock concentration!

You may need a higher concentrated stock solution.



#17059 dilution question help

Posted by Minnie Mouse on 26 February 2009 - 01:25 PM in General Lab Techniques

Thanks for the formula.

Say I have DNA that measured 1 mg/ml concentration and my target concentration is 100ug, 50ul final, how would I do this? I tried using it with the formula below, but it doesn't work very well. How can I get 100ug of 1 mg/ml somehow?

1mg/ml = 1ug/ul

(1ug/ul)*V = (100ug/50ul)* 50ul
(1ug/ul)*V=(2ug/ul)*50ul
V = 100ug/(1ug/ul)
V = 100ul


The final concentration is 2ug/ul which is 2mg/ml... twice as much as the stock concentration!

You may need a higher concentrated stock solution.


Ok, I try with 100ng. If I do the calculation again:

1ug/ul = 1000ng/ul

(1000 ng/ul)*V = (100ng/50ul)*50ul
(1000 ng/ul)*V = (2ng/ul)*50ul
V = (100ng)/(1000 ng/ul)
V = 0.1 ul

Is that correct?


yes, but you cannot measure 0.1ul accurately.

You may need to take 1ul of stock solution and diluted it into a 9ul of buffer/water (1:10 dilution)
Then take a 1ul of this solution and add 49ul of buffer/water.

Hope this may help.



#23905 eHandbook of Receptor Classification and Signal Transduction

Posted by Minnie Mouse on 09 May 2009 - 09:12 PM in Free Stuff

http://www.sigmaaldr.../ehandbook.html



#14133 ELISA protocol (direct, indirect and sandwich)

Posted by Minnie Mouse on 01 February 2009 - 02:13 PM in ELISA and Immunoassay

ELISA protocol from Abcam

Direct ELISA
http://www.abcam.com...g...e&rid=11388

Indirect ELISA
http://www.abcam.com...g...e&rid=11389

Sandwich ELISA
http://www.abcam.com...g...e&rid=11422



#14135 ELISPOT protocol and troubleshooting tips

Posted by Minnie Mouse on 01 February 2009 - 02:15 PM in ELISA and Immunoassay

ELISPOT Protocol
http://www.abcam.com...g...e&rid=11456

Troubleshooting tips
http://www.abcam.com...g...e&rid=11457



#21226 Extended Essay Suggestions..?

Posted by Minnie Mouse on 07 April 2009 - 01:24 PM in Research Idea, Design and Collaboration

Which area of biology interest you?
Zoology, Botany, biochemistry, microbiology, genetics




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