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Minnie Mouse's Content
There have been 136 items by Minnie Mouse (Search limited from 10-April 19)
overexpression or over-expression
upregulation or up-regulation
antitumor or anti-tumor
costimulatory or co-stimulatory
immunohistochemistry or immuno-histochemistry
downregulation or down-regulation
proapoptotic or pro-apoptotic
antiapoptotic or anti-apoptotic
prosurvival or pro-survival
chemoresistance or chemo-resistance
protooncogene or proto-oncogene
Thanks in advance.
1. The permeabilized cancer cells were fixed with 3.7% paraformaldehyde and then permeabilized with saponin.
2. cells were stained with either A or B antibody
3. the secondary conjugate was anti-mouse FITC
The antigen was expressed on the cell surface.
Two monoclonal antibodies (A and B ) against the same antigen were used to stain the cells.
A and B antibodies stained weaker in fixed permeabilized cells than non-fixed cells.
Minnie__s_flow_cytometry_result.ppt 56KB 616 downloads
Thanks in advance.
1. Do I have to calculate average or mean?
2. What Test do I have to use to get p values?
3. When I draw the data above in Excel Bar graph, I want to present average/mean as well as 'deviate'. What deviate do I have to use? standard error(SE) OR standard deviation(SD)?
Thank you so much in advance!![/size]
1. average = mean
2. two-way ANOVA
Hope this may help.
Serum is the product of clotted blood. Blood clots form in response to a cutaneous wound. The growth factors derived from platelet degranulation (primarily PDGF, but also TGF-beta, IGF, FGF, EGF, etc.) are what make your tissue culture cells grow. These factors also drive the early stages of wound healing. Fetal wounds heal more quickly than adult ones, and without scarring. One of the reasons for this is hypothesized to be that the cells activated by fetal serum grow more quickly than those of adults, due to a different balance of cytokines. Thus cells grow more quickly in FCS than in NBCS where they tend to grow bigger and slower.
FCS is the only one of the three that does not contain antibodies.
ECM factors, such as fibronectin, are also present in serum.
-Heat treatment inactivates complement and should always be done with FCS!
These cytokines are not present in BSA.
You may try to search "Minnie Mouse and Bioforum" in Google.com.
The results will show how many posts I have made in Bioforum.