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Minnie Mouse's Content

There have been 136 items by Minnie Mouse (Search limited from 21-September 19)



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#13515 Current Protocol by John Wiley & Son

Posted by Minnie Mouse on 27 January 2009 - 01:34 PM in Tissue and Cell Culture

Current Protocol by John Wiley & Son

Please check link.
https://commerce.inv...44C7DCE18F84CBD



#14133 ELISA protocol (direct, indirect and sandwich)

Posted by Minnie Mouse on 01 February 2009 - 02:13 PM in ELISA and Immunoassay

ELISA protocol from Abcam

Direct ELISA
http://www.abcam.com...g...e&rid=11388

Indirect ELISA
http://www.abcam.com...g...e&rid=11389

Sandwich ELISA
http://www.abcam.com...g...e&rid=11422



#23905 eHandbook of Receptor Classification and Signal Transduction

Posted by Minnie Mouse on 09 May 2009 - 09:12 PM in Free Stuff

http://www.sigmaaldr.../ehandbook.html



#13817 Statistics at Square One, nineth edition

Posted by Minnie Mouse on 29 January 2009 - 02:10 PM in Bioinformatics and Biostatistics

Statistics at Square One, nineth edition

http://www.bmj.com/c...atsbk/index.dtl



#13816 Concepts and Application of Inferential Statistics

Posted by Minnie Mouse on 29 January 2009 - 02:09 PM in Bioinformatics and Biostatistics

Concepts and Application of Inferential Statistics


http://faculty.vassa...ry/webtext.html



#17756 A manual of techniques for separation, detection and recovery of nucleic acids a

Posted by Minnie Mouse on 04 March 2009 - 02:04 PM in Free Stuff

Thank you lactolee



#21460 confusion about 5'UTR

Posted by Minnie Mouse on 09 April 2009 - 09:44 PM in Molecular Cloning

Hi Chicken,

Easter is coming.
Some people are on holiday or are busy finishing the experiments before the holiday.

After Easter Holiday, there will be more people to answer the posts.



#14364 Free Rice

Posted by Minnie Mouse on 03 February 2009 - 05:48 PM in Be a Geek

http://www.freerice.com/index.php

Play Free Rice and help to end the world hunger

My best vocabulary level is 51 :huh:



#14503 BioForum trawling

Posted by Minnie Mouse on 04 February 2009 - 01:00 PM in Chit Chat

There is a "Search" buttom at the top right hand corner and a "Forum Archives" buttom at the top...slightly to the right.

Hope this may help.



#14135 ELISPOT protocol and troubleshooting tips

Posted by Minnie Mouse on 01 February 2009 - 02:15 PM in ELISA and Immunoassay

ELISPOT Protocol
http://www.abcam.com...g...e&rid=11456

Troubleshooting tips
http://www.abcam.com...g...e&rid=11457



#31646 Scientific words

Posted by Minnie Mouse on 04 August 2009 - 03:49 AM in Scientific English

Would someone tell me which words are correct with hyphen, please? :(

overexpression or over-expression
upregulation or up-regulation
antitumor or anti-tumor
costimulatory or co-stimulatory
immunohistochemistry or immuno-histochemistry
downregulation or down-regulation
proapoptotic or pro-apoptotic
antiapoptotic or anti-apoptotic
prosurvival or pro-survival
chemoresistance or chemo-resistance
protooncogene or proto-oncogene


Thanks in advance.



#27241 Research Assistant positions for semi fresh graduates like me

Posted by Minnie Mouse on 21 June 2009 - 08:12 PM in Venting and Counseling

Have you think about working overseas?



#22153 Freedom Rocker - Save yourself from babysitting your gels!

Posted by Minnie Mouse on 17 April 2009 - 03:56 PM in Lab Equipments

Is this machine durable?

I am very interest in this machine, but worry that it may breakdown after two years (after the 2-year warranty).



#21934 different staining intensity

Posted by Minnie Mouse on 15 April 2009 - 08:17 PM in Flow Cytometry

Why is the staining intensity weaker in fixed permeabilized cells than non-fixed cells?

1. The permeabilized cancer cells were fixed with 3.7% paraformaldehyde and then permeabilized with saponin.
2. cells were stained with either A or B antibody
3. the secondary conjugate was anti-mouse FITC


The antigen was expressed on the cell surface.
Two monoclonal antibodies (A and B ) against the same antigen were used to stain the cells.
A and B antibodies stained weaker in fixed permeabilized cells than non-fixed cells.

Attached File  Minnie__s_flow_cytometry_result.ppt   56KB   651 downloads


Why?



Thanks in advance.



#21703 Tumor size statistics in 4 groups of transgenic mice!!

Posted by Minnie Mouse on 13 April 2009 - 02:51 PM in Bioinformatics and Biostatistics

1. Do I have to calculate average or mean?
2. What Test do I have to use to get p values?
3. When I draw the data above in Excel Bar graph, I want to present average/mean as well as 'deviate'. What deviate do I have to use? standard error(SE) OR standard deviation(SD)?

Thank you so much in advance!![/size]


1. average = mean

2. two-way ANOVA

3. SD

Hope this may help.



#20732 Newborn Bovine Serum

Posted by Minnie Mouse on 01 April 2009 - 04:14 PM in Tissue and Cell Culture

Serum is the product of clotted blood. Blood clots form in response to a cutaneous wound. The growth factors derived from platelet degranulation (primarily PDGF, but also TGF-beta, IGF, FGF, EGF, etc.) are what make your tissue culture cells grow. These factors also drive the early stages of wound healing. Fetal wounds heal more quickly than adult ones, and without scarring. One of the reasons for this is hypothesized to be that the cells activated by fetal serum grow more quickly than those of adults, due to a different balance of cytokines. Thus cells grow more quickly in FCS than in NBCS where they tend to grow bigger and slower.
FCS is the only one of the three that does not contain antibodies.
ECM factors, such as fibronectin, are also present in serum.
-Heat treatment inactivates complement and should always be done with FCS!

These cytokines are not present in BSA.

-tfitzwater-




#16673 New Microbiology Guide

Posted by Minnie Mouse on 23 February 2009 - 01:49 PM in -Microbiology and Virology-

I cannot open the link. :)



#14261 Is Google Back too!!

Posted by Minnie Mouse on 02 February 2009 - 07:57 PM in Chit Chat

Google.com is online because it is updating its database. So that we can search information in Bioforum via Google.

You may try to search "Minnie Mouse and Bioforum" in Google.com. :P

The results will show how many posts I have made in Bioforum.



#14137 immunoprecipitation protocol

Posted by Minnie Mouse on 01 February 2009 - 02:29 PM in Protein and Proteomics

Immunoprecipitation protocol by Abcam

http://www.abcam.com...g...e&rid=11375


Troubleshooting tips

http://www.abcam.com...g...e&rid=11382



#19988 Statistics help

Posted by Minnie Mouse on 25 March 2009 - 02:30 PM in Bioinformatics and Biostatistics

two-way ANOVA



#14515 Which journal should I publish?

Posted by Minnie Mouse on 04 February 2009 - 03:19 PM in Journal Discussion - How to Get Papers Published

Thank you, gebirgsziege. :(

This post is pinned.



#17450 More informative error messages

Posted by Minnie Mouse on 02 March 2009 - 01:23 PM in Suggestion

Please check this thread

http://www.protocol-...?showtopic=6486



#14644 immunoprecipitation protocol

Posted by Minnie Mouse on 05 February 2009 - 01:56 PM in Protein and Proteomics

Crosslinking the antibody to the beads protocol

http://www.abcam.com...g...e&rid=11415



#14136 Western Blotting- A beginner Guide

Posted by Minnie Mouse on 01 February 2009 - 02:18 PM in SDS-PAGE and Western Blotting

Western Blotting - A beginner Guide by Abcam

http://www.abcam.com...g...e&rid=11375



#27240 PhD problems

Posted by Minnie Mouse on 21 June 2009 - 08:10 PM in Life as a PhD Student and Postdoc

Can you manage to use your result data to write up a PhD thesis?




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