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Minnie Mouse's Content

There have been 136 items by Minnie Mouse (Search limited from 21-September 19)



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#17611 Tampering With Things Man Was Not Meant to Know

Posted by Minnie Mouse on 03 March 2009 - 04:20 PM in Lab Jokes

17. Avoid working for a psychopath boss.



#13838 all gone??????

Posted by Minnie Mouse on 29 January 2009 - 05:59 PM in Chit Chat

research gate is over, but has anybody visited this molecular station site???? It seems there are some vultures around....... :wacko:

do U remember there was another dude asking for name for a scientist facebook. I wonder what happened to him.


But some are lost, for example toejam, vetticus3, fred33, aimikins (?). Have a poor memory for (nick-)names.


Rhombus, Calvin*, cellcounter, and phage434 may be lost.



#21449 How many of you doing research as a passion not for survial

Posted by Minnie Mouse on 09 April 2009 - 07:56 PM in Chit Chat

How many of you really are doing research as a passion instead of for survival, and really believe what you are doing is going to solve some real problem. I believe over 90% of us will respond "no" including myself.


me, yes ;)

I worked as a volunteer technician for 6 months, then worked as a fully self-funded PhD student for 2 years in the same lab.

If it is not for passion, I would do a MBA instead...
A small investment company offered to pay for my MBA tuition and gave me a brand new BMW if I do MBA and continue working in their company.



#15754 top ten lies by grad students

Posted by Minnie Mouse on 16 February 2009 - 12:50 PM in Lab Jokes

29. I will complete my PhD in less than three years



#14513 top ten lies by grad students

Posted by Minnie Mouse on 04 February 2009 - 03:11 PM in Lab Jokes

19. I have made a big discovery.



#15889 How to make a fake egg?

Posted by Minnie Mouse on 17 February 2009 - 03:27 PM in Chit Chat

10 steps to make a fake egg :P :angry:

http://www.weirdasia...ake-a-fake-egg/



#15895 How to make a fake egg?

Posted by Minnie Mouse on 17 February 2009 - 05:56 PM in Chit Chat

Why would someone bother to make fake egg? :angry:

Isn't it cheaper to buy a real one than making a fake one? :P



#15897 How to make a fake egg?

Posted by Minnie Mouse on 17 February 2009 - 05:57 PM in Chit Chat

genius!


Will you try to make one and eat it? :angry:



#17635 What kind of operating system are you using?

Posted by Minnie Mouse on 03 March 2009 - 07:34 PM in Be a Geek

There are fewer viruses for MacOS than window.



#17615 What kind of operating system are you using?

Posted by Minnie Mouse on 03 March 2009 - 04:52 PM in Be a Geek

your resident technoidiot,

casandra


Same here...

Minnie can't even turn on a Mac computer, nor delete a file in MacOS. :lol:



#27236 Where might they be?

Posted by Minnie Mouse on 21 June 2009 - 07:37 PM in Chit Chat

They may be on holiday together. :o



#23064 Silly things that make Scientists/Researchers happy

Posted by Minnie Mouse on 29 April 2009 - 02:01 PM in Chit Chat

playing with the bubble wrap. POP POP POP ;)

It makes me happy.



#23503 Silly things that make Scientists/Researchers happy

Posted by Minnie Mouse on 05 May 2009 - 02:11 PM in Chit Chat

printing double sided. makes me feel "environmentally friendly" :D


same here :wacko:



#14140 Tips and FAQs about freezing and thawing cells

Posted by Minnie Mouse on 01 February 2009 - 02:39 PM in Tissue and Cell Culture

Freezing cells protocol by Abcam


http://www.abcam.com...g...e&rid=11742



#27239 Re-using Culture flasks

Posted by Minnie Mouse on 21 June 2009 - 08:00 PM in Tissue and Cell Culture

are flasks THAT expensive you would mess up your results - just to recycle them? (taking into account the price of everthing else used leading to that point)

d



Dom,

Wonderful to have you back and talking common sense....your not my long lost brother?

Uncle Rhombus


Well, that all depends on where your funding comes from. I have to beg, borrow and steal just about everything because of a few expensive purchases in the beginning.
But yea, sure they are not THAT expensive to risk messing my results but I didnt know that until now.


I got the same problem during my PhD because I didn't have a sponsor to pay for my expensive PhD training cost :o .
I had to re-use the flasks for 5-10 times...not a good idea...but I did not have enough money to do experiments.

I know Uncle Rhombus will laugh at me. :(



#19735 Reuse DNA spin column

Posted by Minnie Mouse on 23 March 2009 - 01:56 PM in Molecular Biology

thank you, labtek



#14341 Reuse DNA spin column

Posted by Minnie Mouse on 03 February 2009 - 02:08 PM in Molecular Biology

Reuse DNA spin column

Attached File  reuse_DNA_spin_column.pdf   440.14KB   3677 downloads



#21704 Reuse DNA spin column

Posted by Minnie Mouse on 13 April 2009 - 02:55 PM in Molecular Biology

I am confused about the method exactly.

It says to soak in 1 M HCl then rinse in sterile distilled water and equilibriate with Buffer QBT.

When they say rinse do they really mean just rinse or fill the column and keep cetrifuging the water through?

Also same thing about equilibrate. What does that mean exactly.


fill the column and centrifuge to get rid of the water



#14637 Reuse DNA spin column

Posted by Minnie Mouse on 05 February 2009 - 01:19 PM in Molecular Biology

Reuse DNA spin column

Attached File  reuse_DNA_spin_column.pdf   440.14KB   3677 downloads



Thanks Minnie mouse to post my paper (i am one of the author of this paper)


Thank you for publishing this paper. You have saved our money ;)

If you have further tips on reusing the DNA spin column, please post them here? :(

We would love to know :)



#14871 Reuse DNA spin column

Posted by Minnie Mouse on 07 February 2009 - 02:55 PM in Molecular Biology

Thank you for the link, phage434.

Welcome back phage434.



#17459 Strange Science (Research)

Posted by Minnie Mouse on 02 March 2009 - 03:41 PM in Chit Chat

How about female science nerds?



#17458 Kinda angry... partner is getting me down :(

Posted by Minnie Mouse on 02 March 2009 - 03:37 PM in Venting and Counseling

He is probably having depression.
He may be lack of motivation after losing his job.

It may be better to tell him how lucky he is to have a roof.
There are some unemployed people now homeless and live in the street.



#16667 how would you read this?

Posted by Minnie Mouse on 23 February 2009 - 01:32 PM in Chit Chat

if just you could see the H as an N.... and the ! as an L

you know, this whole thing reminded me of the famous dolphin/couple picture. if you show the below picture to some adults they can see a man and woman making love, but if you show this to kids they can only see 7 dolphins.

according to the picture I must have a corrupted mind, it's really hard for me to find all the dolphins. :blink:


I can only see 7 dolphins...this means I am a kid. :(



#20614 Liquid and solid autoclave

Posted by Minnie Mouse on 31 March 2009 - 02:59 PM in General Lab Techniques

Normally, I autoclave buffer and tip boxes together using liquid cycle.



#20836 Liquid and solid autoclave

Posted by Minnie Mouse on 02 April 2009 - 01:07 PM in General Lab Techniques

I would autoclave solid (tips boxes and eppendorf) and liquid (buffers) together using liquid cycle, rather than separate them and run two different cycles.

It would help to reduce greenhouse gases.




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