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Minnie Mouse's Content

There have been 136 items by Minnie Mouse (Search limited from 14-November 18)



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#19735 Reuse DNA spin column

Posted by Minnie Mouse on 23 March 2009 - 01:56 PM in Molecular Biology

thank you, labtek



#19734 Practical Flow Cytometry, Fourth Edition

Posted by Minnie Mouse on 23 March 2009 - 01:54 PM in Flow Cytometry

Thank you, yobou



#19730 We're all wasting our time!!!

Posted by Minnie Mouse on 23 March 2009 - 01:46 PM in Lab Jokes

I also got a paper. :) ;)

Attached File  minnie_paper.PDF   118.28KB   284 downloads

Attached Files




#19258 What is your IQ?

Posted by Minnie Mouse on 18 March 2009 - 12:52 PM in Chit Chat

Do anyone pay for the membership?

I didn't.



#18545 What is your IQ?

Posted by Minnie Mouse on 11 March 2009 - 05:02 PM in Chit Chat

Besides, high IQ is like long legs for a volleyball player. It sure helps, but there are lots and lots of other factors.


EQ Emotion Quotient

CQ "Computer Quotient": ability to use computer :D

GQ "Grammar Quotient" ability to write perfect grammar . I think I am below average on GQ. :D



#18452 Obsolete...?

Posted by Minnie Mouse on 10 March 2009 - 07:33 PM in Venting and Counseling

This is not unexpected -- the PIs responsibilities to a grad student are greater than those to a technician. I'm not being harsh, but it's the truth. Producing a well-trained and successful grad student is more beneficial to a PIs career than is having a successful technician, and failing to produce a successful grad student is worse for the PI. The relationships are totally different, and should be. BTW, I was a lab tech for years, so I know where you're coming from.

You're not obsolete, it's just that your role in the lab has changed.


But aren't it unfair for the technician.
Technician may stay in the lab for >5 years. But PhD student only 3-4 years, master student 2 years.

I used to work in the lab, which values technician more than PhD student.
When the lab is low in budget, the PI would rather cut the PhD's experiment than the technician, because the university did not pay for the training cost for this PhD student.



#18413 Obsolete...?

Posted by Minnie Mouse on 10 March 2009 - 01:40 PM in Venting and Counseling

Why not get into Graduate School?
To become equal to the student.



#17779 What is your IQ?

Posted by Minnie Mouse on 04 March 2009 - 06:14 PM in Chit Chat

135 :lol:


Don't worry. You are still in the top 2% of the world. :o



#17777 What is your IQ?

Posted by Minnie Mouse on 04 March 2009 - 06:02 PM in Chit Chat

EDIT: :o By randomly hitting buttons I got IQ 108.


I answered "C" for every single question and I got a score of 103. :lol:

Then how to get a score of 70. :huh:



#17756 A manual of techniques for separation, detection and recovery of nucleic acids a

Posted by Minnie Mouse on 04 March 2009 - 02:04 PM in Free Stuff

Thank you lactolee



#17754 What is your IQ?

Posted by Minnie Mouse on 04 March 2009 - 01:57 PM in Chit Chat

What is your IQ?

http://www.highiqsociety.org/iq_tests/

My IQ is 143 :o ... but I don't think it is accurate.



#17635 What kind of operating system are you using?

Posted by Minnie Mouse on 03 March 2009 - 07:34 PM in Be a Geek

There are fewer viruses for MacOS than window.



#17615 What kind of operating system are you using?

Posted by Minnie Mouse on 03 March 2009 - 04:52 PM in Be a Geek

your resident technoidiot,

casandra


Same here...

Minnie can't even turn on a Mac computer, nor delete a file in MacOS. :lol:



#17611 Tampering With Things Man Was Not Meant to Know

Posted by Minnie Mouse on 03 March 2009 - 04:20 PM in Lab Jokes

17. Avoid working for a psychopath boss.



#17589 rating

Posted by Minnie Mouse on 03 March 2009 - 01:25 PM in Chit Chat

I also notice it... ELISA and flow cytometry subforum got the rating system but not cell biology forum :)


It is back now.


Thank you, Bioforum



#17474 rating

Posted by Minnie Mouse on 02 March 2009 - 06:55 PM in Chit Chat

This feature was in the old forum, I'm quite sure of that. The geek forum has it (you can rate all the topics over there) and it's new. But for the tech forums, some subforums have and some don't, so it's really intriguing. And at least now we know that moderators are not culpable, there go our escape goats :D ....


I also notice it... ELISA and flow cytometry subforum got the rating system but not cell biology forum :)



#17473 rating

Posted by Minnie Mouse on 02 March 2009 - 06:48 PM in Chit Chat

lol......the same topic that you replied earlier had rating....the Taqman primer and probe.


I would like to know how many posts I'd have to get PM enabled. I was in the middle of giving advice to someone and waiting for the answer, oops.
And I am not such a fervent poster to quickly make the necessary amount.

EDIT: oooh it works.


I think PM is enabled when you reach "two blue squares".



#17461 Trained

Posted by Minnie Mouse on 02 March 2009 - 03:50 PM in Lab Jokes

This sounds like my dog.

He sits beside his snack box patiently and we give him a snack.

Sometimes, he would sit for half an hour.



#17459 Strange Science (Research)

Posted by Minnie Mouse on 02 March 2009 - 03:41 PM in Chit Chat

How about female science nerds?



#17458 Kinda angry... partner is getting me down :(

Posted by Minnie Mouse on 02 March 2009 - 03:37 PM in Venting and Counseling

He is probably having depression.
He may be lack of motivation after losing his job.

It may be better to tell him how lucky he is to have a roof.
There are some unemployed people now homeless and live in the street.



#17450 More informative error messages

Posted by Minnie Mouse on 02 March 2009 - 01:23 PM in Suggestion

Please check this thread

http://www.protocol-...?showtopic=6486



#17063 Seeding volume in cell culture flasks

Posted by Minnie Mouse on 26 February 2009 - 01:41 PM in Cell Biology

In my hands, no.

The reason is when you lay the flask on its side during incubation, the amount of media flows too closely to the neck which could lead to contamination.

For suspension cultures, the largest volume I've used is 16 mL.
If you need larger volume, try a spinner flask or roller bottle.



yes your are correct i used only 15ml but i saw that we can make upto 40ml
i want to try...as it is not possible to keep the flask flat ...could it be possible to stand the flask ?
do the cells still be happy in that conditions with less length?


I don't think your cell will be happy.
If you stand the flask, the cell will accumulate at the bottom of the flask and reduce the area for them to grow.
The cells at the very bottom will not get nutrient from the medium. Unless you gently shake the flask every hour.

This is why people invented rolling bottle.

Hope this may help.



#17060 Toxicity Assay

Posted by Minnie Mouse on 26 February 2009 - 01:31 PM in Cell Biology

I think you can treat HL60 cells immediately after seeding.
They don't need to recover from trypsinize nor waiting them to adhere to the bottom of the plate/flask.
As long as you don't spin them too hard before seeding them.

Hope this may help.



#17059 dilution question help

Posted by Minnie Mouse on 26 February 2009 - 01:25 PM in General Lab Techniques

Thanks for the formula.

Say I have DNA that measured 1 mg/ml concentration and my target concentration is 100ug, 50ul final, how would I do this? I tried using it with the formula below, but it doesn't work very well. How can I get 100ug of 1 mg/ml somehow?

1mg/ml = 1ug/ul

(1ug/ul)*V = (100ug/50ul)* 50ul
(1ug/ul)*V=(2ug/ul)*50ul
V = 100ug/(1ug/ul)
V = 100ul


The final concentration is 2ug/ul which is 2mg/ml... twice as much as the stock concentration!

You may need a higher concentrated stock solution.


Ok, I try with 100ng. If I do the calculation again:

1ug/ul = 1000ng/ul

(1000 ng/ul)*V = (100ng/50ul)*50ul
(1000 ng/ul)*V = (2ng/ul)*50ul
V = (100ng)/(1000 ng/ul)
V = 0.1 ul

Is that correct?


yes, but you cannot measure 0.1ul accurately.

You may need to take 1ul of stock solution and diluted it into a 9ul of buffer/water (1:10 dilution)
Then take a 1ul of this solution and add 49ul of buffer/water.

Hope this may help.



#16947 dilution question help

Posted by Minnie Mouse on 25 February 2009 - 07:32 PM in General Lab Techniques

Thanks for the formula.

Say I have DNA that measured 1 mg/ml concentration and my target concentration is 100ug, 50ul final, how would I do this? I tried using it with the formula below, but it doesn't work very well. How can I get 100ug of 1 mg/ml somehow?

1mg/ml = 1ug/ul

(1ug/ul)*V = (100ug/50ul)* 50ul
(1ug/ul)*V=(2ug/ul)*50ul
V = 100ug/(1ug/ul)
V = 100ul


The final concentration is 2ug/ul which is 2mg/ml... twice as much as the stock concentration!

You may need a higher concentrated stock solution.




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