I agree with Thelymitra pulchella. Your protein may be aggregating into inclusion body form. For avoiding this situation, you may need some advises, such as 1) fusion of the target protein with a more soluble partner, typically a bacterial protein; 2) co-expression of folding catalysts and chaperones; 3) expression under culture conditions which reduce the translation rates or affect the intracellular environment; and 4) modification of the protein sequence.
Submit your paper to J Biol Methods today!
hops proteon's Content
There have been 3 items by hops proteon (Search limited from 30-May 19)