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-Kay's Content

There have been 6 items by -Kay (Search limited from 29-May 19)


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#100746 Protein streaking on Blue Native-PAGE

Posted by -Kay on 15 February 2011 - 01:42 PM in Protein and Proteomics

I understand. Thanks!!



#100693 Protein streaking on Blue Native-PAGE

Posted by -Kay on 15 February 2011 - 07:22 AM in Protein and Proteomics

Sorry, I had a slow moment... Won't adding b-mercaptoethanol denature my proteins, making it pointless for a NativePAGE gel?

I'm trying to determine the size of my proteins in their native state. Since gel filtration didn't work, my next option was to try this Novex NativePAGE gel system (charges the proteins with coomassie). Because of the streaking, it hasn't been helpful either.



#100684 Protein streaking on Blue Native-PAGE

Posted by -Kay on 15 February 2011 - 06:36 AM in Protein and Proteomics

Thanks so much! I'll try that out and see how it works out



#100590 Protein streaking on Blue Native-PAGE

Posted by -Kay on 14 February 2011 - 11:15 AM in Protein and Proteomics

how much would you recommend I add?



#99959 Protein streaking on Blue Native-PAGE

Posted by -Kay on 08 February 2011 - 09:26 AM in Protein and Proteomics

Yes, they streak all the way back to the origin, and I've tried reducing the sample concentration.

I'm stumped



#99298 Protein streaking on Blue Native-PAGE

Posted by -Kay on 01 February 2011 - 12:38 PM in Protein and Proteomics

Hi, all!
I have been running blue Native PAGE gels using 3-12% bis-tris gels. I keep getting protein streaking, and no matter what I do, it won't go away. I have reduced the salt concentration of my samples, used the 5% G-250 coomassie sample additive, and spun the samples the remove any particulates, and reduced the amount of protein in my but the streaking endures. Any thoughts as to what could be causing it?




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