I understand the science behind tetramer preparation but I am not sure how to calculate the specific volume of antibody to be used. I have read the NIH preparation method but they do not have calculations/formulas for their guidelines. Is there a good rule of thumb formula/ratio of antibody to monomer to use (like using 1ug of antibody to 4ug of monomers)?
EDIT: I see they say "The actual amount of streptavidin to be added depends both on the molecular weight of the monomer as well as the percent biotinylation. It is necessary to divide this amount by ten to arrive at the amount to be added per time interval."
How do you find this information? Are class I monomers produced with different MW, differing from batch to batch?