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Cloning Related Discussions
  1. Favorite polymerase for general cloning? - (reply: 1)
  2. restriction enzyme pcr cloning - (reply: 1)
  3. polb cloned in pET not expressed - (reply: 2)
  4. Restriction Digest with pGEMT for TA cloning - (reply: 14)
  5. Cloning of big inserts - (reply: 1)
  6. TA cloning..Urgent Help!! - (reply: 4)
  7. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
  8. Subcloning issue - (reply: 2)
  9. pGEM T Easy subcloning - (reply: 1)
  10. Target gene cloning - (reply: 4)
  11. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
  12. Problem with possible leaky expression in cloning - (reply: 4)
  13. Luciferase assay cloning - (reply: 2)
  14. Problem with restriction digestion of cloned PCR product - (reply: 5)
  15. SLIC Cloning Failure - (reply: 2)
  16. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  17. Polymerase mixture for blunt ended fragments - (reply: 2)
  18. Cloned gene in pET system not being expressed - (reply: 10)
  19. Problem with subcloning genescript synthesized genes - (reply: 2)
  20. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
  21. Problem with double digest cloning - (reply: 3)
  22. How good is your cloning efficiency? - (reply: 2)
  23. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
  24. Cloning a 17 kb contruct - (reply: 2)
  25. Cloning of an unknown gene - (reply: 3)
  26. Cloning of an unknown gene - (reply: 3)
  27. cloning problems using in-fusion kit - (reply: 5)
  28. Cloning - Ligation problem - (reply: 3)
  29. How to clone a 2A peptide between two ORFs? - (reply: 2)
  30. TA Cloning - (reply: 11)
  31. Troubleshooting with cloning - (reply: 2)
  32. Suitable ratio of vector and insert in cloning - (reply: 5)
  33. Cloning a big insert in a small vector - (reply: 2)
  34. Cloning purified PCR product into cells - (reply: 5)
  35. Gateway cloning not working. - (reply: 7)
  36. Insert direction in TA cloning - (reply: 2)
  37. Problem with stable clone - (reply: 2)
  38. Should I proceed with clone selection? - (reply: 2)
  39. Can I loose the protein of interest from a stable clone? - (reply: 2)
  40. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
  41. Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
  42. Sticky and blunt end ligation in single reaction? - (reply: 2)
  43. How can we expand molecular cloning further? - (reply: 3)
  44. Molecular cloning using an ezh2 insert - (reply: 2)
  45. Subtle change in insert size after cloning - (reply: 6)
  46. lactic acid bacteria - cloning - (reply: 2)
  47. Can't isolate plasmid from cloned E.coli - (reply: 4)
  48. cloning issues - (reply: 3)
  49. Is important the direction of IRES-GFP in cloning? - (reply: 1)
  50. Cloning inserts: forward and reverse orientation - (reply: 4)
  51. What are the error-rates of the TA cloning Kits? - (reply: 8)
  52. What sorts of cloning strategies are available for creating inducible knockouts - (reply: 2)
  53. Can mutations be created during cloning? - (reply: 7)
  54. What is the full name for TA cloning - (reply: 4)
  55. cloning problem - (reply: 2)
  56. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
  57. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
  58. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
  59. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
  60. primers for cloning - (reply: 1)
  61. Cloning contamination - (reply: 3)
  62. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  63. Problem shrna cloning, bad sequencing results - (reply: 1)
  64. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  65. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
  66. Blunt end cloning - (reply: 12)
  67. Molecular cloning - (reply: 5)
  68. Nuclease free water for cloning - (reply: 8)
  69. Directional cloning 2bps overhang - (reply: 3)
  70. filling the digested vector and insert for blunt end cloning - (reply: 2)
  71. OVA cDNA clone? - (reply: 2)
  72. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
  73. Purpose of PolyA in cloning - (reply: 1)
  74. Cloning into pVIVO2 vector - (reply: 8)
  75. Cloning worked, but the expression vector is weird - (reply: 6)
  76. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
  77. Ubiquitin promoter cloning - (reply: 1)
  78. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  79. Is this counter-productive cloning or not? - (reply: 8)
  80. Failed cloning for 1month - TOPO - (reply: 7)
  81. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
  82. Which method for DNA purification b4 cloning - (reply: 4)
  83. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
  84. Failed TOPO cloning - what happened? - (reply: 9)
  85. TOPO cloning after Phusion-PCR - (reply: 1)
  86. Gene cloning with unknown sequence - (reply: 6)
  87. Failed cloning - different readings - (reply: 10)
  88. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
  89. Cloning pDsRed-Monomer-N1 - (reply: 1)
  90. Annexin V clone - (reply: 2)
  91. Please comment on my Cloning Plan - (reply: 1)
  92. Cloning problem still not solved - (reply: 2)
  93. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  94. cloning pEGFP-N1 - (reply: 1)
  95. Cloning beginner and clueless! - (reply: 1)
  96. Cloning Problems - (reply: 2)
  97. Different ways to ensure the successful cloning? - (reply: 2)
  98. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
  99. Sticky and blunt ligation issue - (reply: 6)
  100. cloning - (reply: 7)
  101. Help~ Incorrect constructs in cloning - (reply: 3)
  102. Blunt end is ligating to sticky end? - (reply: 2)
  103. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
  104. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
  105. Sticky to Blunt end (Hypothetical) - (reply: 2)
  106. Do genes always need to be cloned in the "right direction"? - (reply: 3)
  107. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  108. Cloning and expression problem - (reply: 5)
  109. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  110. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  111. cloning help; high salt level in vector - (reply: 4)
  112. problem with gateway cloning: missing part of insert - (reply: 3)
  113. Type of polymerases compatible for TA cloning - (reply: 7)
  114. Molecular Cloning Sambrook - (reply: 1)
  115. golden gate cloning - (reply: 3)
  116. types of dna cloning methods - (reply: 2)
  117. excision of fragment during cloning - (reply: 4)
  118. Unusual cloning issue... anyone else?? - (reply: 4)
  119. Sequencing of the cloned plasmid - (reply: 5)
  120. Cloning synthesised genes - (reply: 1)
  121. 4 way cloning into TA Vector - (reply: 10)
  122. Neeed Help , cloning Confirmation ?! - (reply: 5)
  123. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
  124. Newbie to smaI blunt end cloning! - (reply: 1)
  125. What part of the promoter to clone - (reply: 2)
  126. gene cloning, where to start? - (reply: 1)
  127. TA Cloning of ds cDNA - (reply: 6)
  128. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
  129. Cloning cDNA construct into E.Coli. - (reply: 4)
  130. Cloning large transgenes - (reply: 2)
  131. In-fusion HD cloning - (reply: 5)
  132. Few colonies frm sticky end cloning - (reply: 3)
  133. Problem with cloning - (reply: 7)
  134. Too many negative clones using Directional TOPO Cloning - (reply: 5)
  135. Designing cloning primers for DNMT - (reply: 2)
  136. Cloning advice - (reply: 3)
  137. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  138. Negative control antibodies (Anti-mouse C5 Clone BB5.1) - (reply: 1)
  139. Cloning large fragments - (reply: 9)
  140. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
  141. Cloning Mitochondrial Targeting Sequence - (reply: 4)
  142. problem in cloning - (reply: 2)
  143. TA cloning: ligation problem or toxic construct? - (reply: 4)
  144. Designing PCR Primers for cloning - (reply: 17)
  145. Including ATG in cloning? - (reply: 1)
  146. gene cloning - (reply: 1)
  147. Cloning repetitive sequences in mammalian cells - (reply: 3)
  148. Truncated gene Cloning - (reply: 1)
  149. Mutation in cloning - (reply: 6)
  150. Understand the basics of molecular cloning - (reply: 6)
  151. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
  152. what is the ORFeome clone? - (reply: 2)
  153. rescue cloning of a transposon mutant - (reply: 2)
  154. problem in cloning PCR primer design with restriction site - (reply: 4)
  155. Cloning at 6 kb gene - (reply: 3)
  156. Vector insert ratio in cloning - (reply: 3)
  157. polymerase to use for cloning - (reply: 4)
  158. Oligo insert cloning - (reply: 3)
  159. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  160. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  161. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
  162. Single RE site cloning - (reply: 3)
  163. Bisulfite PCR and cloning - (reply: 5)
  164. Cloning not working, all steps checked, clueless - (reply: 1)
  165. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
  166. Cloned but not expressing! - (reply: 8)
  167. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  168. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
  169. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
  170. TOPO TA CLONING - LIGATION STEP - (reply: 9)
  171. pEGFP C-1/N-1 Cloning - (reply: 11)
  172. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
  173. Problem with cloning - what is wrong? - (reply: 6)
  174. cloning: band at different site than desired after RE digestion - (reply: 11)
  175. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
  176. cloning pcr insert into plasmid - (reply: 2)
  177. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
  178. molecular cloning - (reply: 7)
  179. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  180. Another cloning hell....no transformants - (reply: 1)
  181. TOPO TA Cloning Troubleshooting - (reply: 1)
  182. TOPO TA Cloning - (reply: 1)
  183. No insert in subcloning - (reply: 3)
  184. Unable to clone in E. coli cells - (reply: 3)
  185. cloning into pPICZalphaA fails - (reply: 4)
  186. molecular cloning failure - (reply: 2)
  187. cloning antisense promoter in pGL3Basic - (reply: 2)
  188. Help - Troubleshooting cloning into pET28 - all screens positive, but sequencing - (reply: 2)
  189. Explanation of "In-Frame" cloning? - (reply: 6)
  190. Cloning Problem! I need help - (reply: 3)
  191. Help designing primers for use in In-fushion cloning - (reply: 4)
  192. Repeat of desired mutation in cloning product - (reply: 1)
  193. cloning a gene in to a plasmid - (reply: 5)
  194. recognition sites and cloning? - (reply: 2)
  195. question about PCR and cloning - (reply: 1)
  196. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  197. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
  198. Making a qPCR standard amplicon from cloning, why? - (reply: 4)
  199. help ,blunt end ligation - (reply: 4)
  200. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
  201. Subcloning - RE digest and ligation and transformation - (reply: 5)
  202. Making DNA Linkers for cloning purposes - (reply: 1)
  203. cloning short genomic DNA fragments - (reply: 1)
  204. How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
  205. cloning to pET28a - (reply: 1)
  206. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  207. CDS CLONING QUERY - (reply: 5)
  208. unable to amplify an infectious retrovirus clone - (reply: 4)
  209. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
  210. Cloning partial CDS - (reply: 5)
  211. Cloning problem into pGEMT-easy - (reply: 2)
  212. Cloning problem! - (reply: 8)
  213. Plasmid clone in BSP - (reply: 1)
  214. molecular cloning - (reply: 3)
  215. cloning without phosphorylation - (reply: 7)
  216. Cloning genetic sequences of proteins. - (reply: 1)
  217. should I do blunt end my pcr product before ligation - (reply: 2)
  218. Help with CGL gene cloning problems - (reply: 1)
  219. PUAST sub - cloning problem - (reply: 3)
  220. Cloning Problem - Ligation or Transformation - (reply: 4)
  221. Bisulfite cloning... reading material - (reply: 1)
  222. cloning into Pgem t easy - (reply: 1)
  223. I can´t obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
  224. cloning of a long gene using cDNA - (reply: 1)
  225. Isolation of a Gene of Interest for Subcloning - (reply: 8)
  226. Cloning into inducible vector but no expression - (reply: 1)
  227. weird problem in cloning!! - (reply: 4)
  228. Cloning HELP- Possible Primer issues - (reply: 6)
  229. Strain requirements for routine M13 phagemid cloning? - (reply: 1)
  230. Cloning in pETDuet-1 - (reply: 3)
  231. PCR cloning...help! - (reply: 5)
  232. Problems with blunt end ligation - (reply: 3)
  233. cloning problem - (reply: 2)
  234. problem in cloning involving partial digestion - (reply: 1)
  235. Design primers for expression cloning - (reply: 11)
  236. which software to draw the scheme of cloning strategy - (reply: 5)
  237. Blunt end ligation insert:vector ratio - (reply: 1)
  238. Has anyone ever had a large insert when subcloning? Mine is 4.3kb? - (reply: 2)
  239. Impact of primer sequence on TA cloning - (reply: 3)
  240. cloning an AT rich gene - (reply: 3)
  241. Cloning two genes in the same plasmid - (reply: 4)
  242. Problem: Cloning DNA shuffled band - (reply: 2)
  243. Transformation /Cloning Problem - (reply: 3)
  244. a robust multi-gene cloning method - (reply: 2)
  245. strange subcloning problem - (reply: 9)
  246. Cloned sequence is not predicted size by western blot - (reply: 1)
  247. good tutorials/protocols/books on gene cloning - (reply: 2)
  248. Cloning problem using pET32a vector - (reply: 3)
  249. Fixing SNPs after cloning - (reply: 5)
  250. emty vector cloning - (reply: 3)
  251. Double digest after cloning doesn't give gene - (reply: 6)
  252. Blunt end PCR product ligation - (reply: 1)
  253. strange cloning problem - (reply: 9)
  254. need help with subcloning !! - (reply: 2)
  255. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
  256. cloning with nested pcr - (reply: 3)
  257. cloning blues! - (reply: 12)
  258. Gateway cloning vector compatibility - (reply: 2)
  259. Cloning of IL8-promoter - (reply: 3)
  260. Cloning promoter (overlap with another gene!) - (reply: 3)
  261. Cloning a promoter - (reply: 2)
  262. Cloning two fragments into a vector - (reply: 3)
  263. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
  264. Problem with Blunt end ligation - (reply: 4)
  265. Cloning from a pGEMT Easy vector - (reply: 3)
  266. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  267. cloning problem - (reply: 9)
  268. Cloning genes into plant vector - (reply: 1)
  269. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
  270. Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
  271. Cloning App Released from Life Technologies - (reply: 2)
  272. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
  273. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  274. Blunt end cloning of 4Kb product - (reply: 3)
  275. competentce cell, cloning - (reply: 2)
  276. sequential cloning of multiple PCR products - how to do? (reply: 3)
  277. Cloning large gene - (reply: 3)
  278. Glycosilated peptide's cDNA cloning - (reply: 3)
  279. pWM91 cloning problem - (reply: 4)
  280. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
  281. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  282. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
  283. Cloning into lentiviral vector - (reply: 11)
  284. cloning failure help plzzz - cloning (reply: 1)
  285. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  286. PCR cloning problem - could not get colony (reply: 4)
  287. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  288. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  289. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  290. Cloning and expressing a mystery 71KDa protein - (reply: 9)
  291. Insert magically shortening??? - Cloning issues (reply: 2)
  292. Subcloning Problem - (reply: 3)
  293. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  294. cloning problem... - (reply: 5)
  295. cDNA clone - (reply: 1)
  296. miRNA cloning - (reply: 2)
  297. PCR: Cloning Primers - (reply: 8)
  298. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  299. Cloning does not work - (reply: 4)
  300. Help needed with subcloning problem - (reply: 6)
  301. Mouse huntingtin clone - (reply: 1)
  302. BLUNT CLONING With EcoNI - (reply: 1)
  303. Designing a multiple cloning site - (reply: 5)
  304. blunt and sticky end clonning temperature - (reply: 2)
  305. Most common mistakes - What mistakes are made during cloning? (reply: 7)
  306. Cloning direction / orientation? - (reply: 2)
  307. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
  308. optimal size for 3'UTR cloning - miRNA and 3'UTR cloning (reply: 2)
  309. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
  310. Cloning of ITS region - (reply: 6)
  311. SmaI blunt end ligation problem... - (reply: 3)
  312. Cell dilution cloning - Cells cloning (reply: 2)
  313. Why shouldn't you clone using expression bacteria - (reply: 6)
  314. metylated cloning vector - restriction digest of the insert (reply: 2)
  315. Secretion cloning vectors - (reply: 1)
  316. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
  317. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
  318. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
  319. cloning of race products - (reply: 1)
  320. Advice on amount of starting template for subcloning - (reply: 3)
  321. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
  322. cloning a 34kb insert - please suggest good vectors (reply: 1)
  323. Cloning issue - (reply: 3)
  324. Comment of this cloning strategy - (reply: 3)
  325. Cloning Problem: HSP90AA1 - Cloning Problem: HSP90AA1 (reply: 1)
  326. cloning a large insert - (reply: 5)
  327. Transposase contamination in cloning - (reply: 7)
  328. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  329. Cloning of a gene containing 31 exon - (reply: 1)
  330. cloning help - (reply: 3)
  331. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
  332. weird bands after minipreps - molecular cloning (reply: 2)
  333. purification of PCR product for cloning in a vector - (reply: 3)
  334. Cloning a foreign gene into E-coli - (reply: 1)
  335. subcloning into bacterial expression vector - (reply: 1)
  336. cloning into F1 ori of expression vector??? - (reply: 1)
  337. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  338. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  339. Cloning a Promoter - (reply: 3)
  340. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  341. glycerol stock preparation of recombinant clone - (reply: 2)
  342. Cloning - (reply: 1)
  343. Anyone make their own PCR cloning vector? - (reply: 5)
  344. Antibody gene cloning - (reply: 3)
  345. Which cloning kit should I use - (reply: 2)
  346. Topo cloning or pGEM-T easy ? - (reply: 3)
  347. Cloning the coding region of a protein - (reply: 4)
  348. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
  349. ta cloning problem - (reply: 2)
  350. Help: Cloning a 60KB fragment - (reply: 3)
  351. Cloning Problems/ Help - (reply: 2)
  352. clone GENE RT-PCT PRIMERS - cloning genes (reply: 1)
  353. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
  354. Molecular Cloning od Recombinant DNA - (reply: 3)
  355. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  356. obtain a full length cDNA clone WITH RT-PCR - Cloning (reply: 2)
  357. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  358. TOPO cloning problem - (reply: 4)
  359. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
  360. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  361. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  362. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
  363. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  364. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
  365. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
  366. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
  367. Cloning before sequencing- is it necessary??? - (reply: 13)
  368. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
  369. colony PCR for subcloning gene - (reply: 1)
  370. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  371. cloning of a 20 kb insert - (reply: 2)
  372. cloning of an operon - (reply: 2)
  373. Cloning problem - (reply: 2)
  374. Need advice on cloning very small insert into a vector - (reply: 3)
  375. A cloning nightmare - (reply: 8)
  376. Cloning problems i.e. Ligation - (reply: 2)
  377. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  378. cloning problem - (reply: 5)
  379. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  380. Blunt end filling - Blunt end filling (reply: 1)
  381. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
  382. TOPO Cloning - (reply: 5)
  383. blunt ligation - (reply: 4)
  384. DNA Sequencing / Cloning ? - (reply: 5)
  385. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  386. Cloning <100 bp fragments into a vector - (reply: 7)
  387. Cloning <100 bp fragments into a plasmid - (reply: 7)
  388. HpaI blunt end cloning - (reply: 1)
  389. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
  390. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
  391. Cloning in pCR2.1 plasmid - (reply: 7)
  392. Cloning a 3.5Kb fragment for sequencing - P-gem T-easy. (reply: 2)
  393. Advice on Cloning Strategy - (reply: 4)
  394. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
  395. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
  396. Ligation of Blunt PCR Product - (reply: 1)
  397. Cloning Help!! - It is getting very annoying... (reply: 13)
  398. sequence errors created during cloning - (reply: 6)
  399. PCR & Cloning - (reply: 6)
  400. Help cloning large insert into large vector - (reply: 7)
  401. blunt end ligation problem - Self ligation and truncate (reply: 1)
  402. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  403. Cloning: Randomly recombined Plasmids? - (reply: 6)
  404. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
  405. Cloning problems - (reply: 7)
  406. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  407. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  408. Blunt end ligation - Cant get ligation to work (reply: 4)
  409. blunt ligation - (reply: 1)
  410. please help!!cloning problems.. - (reply: 4)
  411. Cloning - PCR and Cloning problem (reply: 3)
  412. problem with cloning of dsred plz help - vector cut seems fine, still religation oocurs (reply: 13)
  413. cloning of GroEL - (reply: 3)
  414. Problems with High Background with Gateway Cloning - (reply: 1)
  415. blunt end ligation - how to solve the blunt end ligation?? (reply: 5)
  416. Removal of 5´ overhangs to form blunt ends - (reply: 1)
  417. problem with pcr cloning from mouse cDNA - (reply: 2)
  418. QUESTION about cloning - (reply: 5)
  419. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
  420. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  421. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  422. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  423. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  424. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
  425. TOPO TA cloning advice - (reply: 4)
  426. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  427. I have cloning problems with pGEM-T Easy - (reply: 5)
  428. blunt end cloning - (reply: 4)
  429. How can multiple cloning sites (MCS) NOT disrupt lacZ gene? - (reply: 2)
  430. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  431. How to retrieve precursor miRNA sequence with extra bases for cloning - (reply: 5)
  432. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  433. blunt-end / 3´A overhang cloning - (reply: 1)
  434. bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
  435. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  436. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  437. cloning - (reply: 6)
  438. Can't seem to do a simple cloning - (reply: 2)
  439. Cloning into pIBV5-His (not working) - (reply: 1)
  440. Cloning from cDNA - the wrong gene?! - (reply: 2)
  441. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
  442. circular or linear pDNA for stable clone generation? - (reply: 1)
  443. TOPO TA cloning problem - (reply: 12)
  444. cloning problem - (reply: 2)
  445. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
  446. serious problem in cloning - (reply: 2)
  447. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  448. Cloning using BamH1 and HindIII - (reply: 8)
  449. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
  450. TA cloning necessary? - (reply: 2)
  451. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  452. cloning - how to clone a gene having stop codons (reply: 4)
  453. Cloning advice needed - (reply: 4)
  454. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  455. subcloning problem - inframe fusion (reply: 1)
  456. ta cloning of pfu amplified fragment - (reply: 9)
  457. Double digestion and cloning - (reply: 2)
  458. cloning of long fragment - (reply: 1)
  459. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
  460. cloning of cellulose synthase gene - (reply: 1)
  461. problem in ligation and cloning - (reply: 1)
  462. cloning of small double strande DNA - I NEED HELP (reply: 3)
  463. Please Help: human Sox2, Oct 4 promoter region cloning - (reply: 2)
  464. concentration for cloning - (reply: 1)
  465. cDNA clone - (reply: 1)
  466. cloning of non-coding region for protein expression - protein expression (reply: 1)
  467. Insert Sequence Problem in Cloning - (reply: 2)
  468. insert sequence in clone - (reply: 6)
  469. Help: incompatible RE site subcloning - (reply: 1)
  470. need help cloning cdna of a known protein - (reply: 1)
  471. cloning in pET 30 B - (reply: 1)
  472. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
  473. Trouble with subcloning - (reply: 6)
  474. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
  475. Cloning Problem - (reply: 3)
  476. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
  477. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  478. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  479. Promoter Cloning for Luciferase Assay - (reply: 3)
  480. having the wrong vector in my clone - (reply: 3)
  481. cloning protocol - (reply: 1)
  482. Problem cloning bisulfite PCR BSP product - (reply: 6)
  483. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  484. problem with my clone - (reply: 3)
  485. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
  486. cloning in Bacteroides - (reply: 9)
  487. Cloning woes - Running out of ideas! - (reply: 7)
  488. Insert problems with TA cloning - (reply: 1)
  489. Unstable clone? - (reply: 1)
  490. Problems facing in cloning with pBI121 vector - (reply: 1)
  491. Cloning into pGem - Problems with 5kb insert (reply: 3)
  492. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  493. Free trial for BxSeqTools: Online Sequence Analysis and Cloning software - free trial for BxSeqTools: Online Sequence Analysis and Cloning (reply: 1)
  494. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  495. lentiviral system: cloning strategy - (reply: 2)
  496. Difficulties cloning by PCR - (reply: 5)
  497. problem with subcloning, please help! - (reply: 8)
  498. Analyse mutant clone - (reply: 7)
  499. Cloning by multiple oligo ligation - (reply: 2)
  500. anyone working currently on gateway cloning???? - (reply: 1)
  501. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  502. Cheap cloning options - any ideas????? (reply: 2)
  503. Subcloning - (reply: 3)
  504. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  505. Subcloning into pMIR-REPORT-luc - (reply: 7)
  506. Cloning big fragment into smaller vector - (reply: 3)
  507. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  508. Difficulty cloning vector with multiple Lox sites - (reply: 2)
  509. Transcription of double blunt end Clone - (reply: 2)
  510. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  511. Gateway cloning - (reply: 8)
  512. T A cloning problems - (reply: 3)
  513. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  514. Blunt-end cloning problem - (reply: 6)
  515. problem in gene cloning - (reply: 4)
  516. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  517. can't make the cloning work - (reply: 3)
  518. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
  519. cloning help - (reply: 9)
  520. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  521. gateway cloning - (reply: 3)
  522. Purifying and cloning 69nt product - (reply: 5)
  523. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
  524. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
  525. A gateway cloning expression vector question - (reply: 1)
  526. Promoter/gene cloning question - (reply: 7)
  527. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  528. insert is missing after TA cloning - TA cloning (reply: 6)
  529. cloning problem - molecular cloning (reply: 1)
  530. Cloning problems - 16Kb and 1.7Kb cloning problems (reply: 5)
  531. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
  532. wrong bands after T-vector cloning - (reply: 3)
  533. Help with cloning - (reply: 6)
  534. Help for T-A cloning - (reply: 15)
  535. No colony for oligo cloning - (reply: 9)
  536. blunt end ligation problem - (reply: 2)
  537. blunt ligation - (reply: 3)
  538. pENTR TOPO cloning - (reply: 1)
  539. yet one more cloning problem - (reply: 7)
  540. cloning miRNA library - (reply: 4)
  541. olligo annealing for shRNA cloning - (reply: 20)
  542. cloning issue - (reply: 1)
  543. Cloning dilemma - (reply: 3)
  544. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  545. Cloning Problems! Need Help! - (reply: 3)
  546. in frame problem after 3 months of cloning! - can I stil use my construct (reply: 5)
  547. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  548. cloning problem - (reply: 3)
  549. Cloning Conundrum - (reply: 7)
  550. TOPO cloning problem - (reply: 15)
  551. Please Help with Lentiviral Cloning - (reply: 1)
  552. Help with cloning!. - Cloning problems (reply: 20)
  553. Cloning PCR fragment - (reply: 1)
  554. molecular cloning - (reply: 2)
  555. DNA cloning - (reply: 4)
  556. Who can explain this? Weird cloning problem.... - (reply: 6)
  557. Help cloning!!! - As usual cloning is not working! (reply: 11)
  558. pSuper cloning problems - (reply: 1)
  559. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
  560. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  561. Receptor discovery/cloning - (reply: 1)
  562. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
  563. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  564. annoying PCR cloning problem (season 2) - (reply: 5)
  565. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  566. an annoying cloning - (reply: 9)
  567. Controls for TOPO cloning? - (reply: 1)
  568. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
  569. Problems cloning Hairpin vector - (reply: 3)
  570. different digestion pattern for same clone - (reply: 4)
  571. cloning trouble - (reply: 15)
  572. cDNA cloning problem - (reply: 1)
  573. How Gateway cloning system works? - (reply: 1)
  574. ds-oligo cloning/ligation issues - (reply: 18)
  575. Cloning multiple miR30 based shRNAs into a Lenti - (reply: 2)
  576. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  577. Help with cloning a plasmid that contains repeats! - (reply: 2)
  578. questions about TOPO cloning - (reply: 7)
  579. Subcloning into pBluescript - (reply: 6)
  580. How to control cloned cDNA expression level in cells? - (reply: 8)
  581. Cloning a labeled PCR product - (reply: 1)
  582. PCR and cloning - (reply: 14)
  583. Cloning trouble - (reply: 5)
  584. Problem cloning oligo into double digested vector - (reply: 9)
  585. cloning 1052bp fragment - no colonies (reply: 11)
  586. cloning problems - empty vector (reply: 3)
  587. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  588. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)