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Cloning Related Discussions
  1. Troubleshooting with restriction enzymes during cloning - (reply: 4)
  2. Favorite polymerase for general cloning? - (reply: 3)
  3. restriction enzyme pcr cloning - (reply: 1)
  4. polb cloned in pET not expressed - (reply: 2)
  5. Restriction Digest with pGEMT for TA cloning - (reply: 19)
  6. Cloning of big inserts - (reply: 2)
  7. TA cloning..Urgent Help!! - (reply: 4)
  8. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
  9. Subcloning issue - (reply: 2)
  10. pGEM T Easy subcloning - (reply: 1)
  11. Target gene cloning - (reply: 4)
  12. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
  13. Problem with possible leaky expression in cloning - (reply: 4)
  14. Luciferase assay cloning - (reply: 2)
  15. Problem with restriction digestion of cloned PCR product - (reply: 5)
  16. SLIC Cloning Failure - (reply: 2)
  17. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  18. Polymerase mixture for blunt ended fragments - (reply: 2)
  19. Cloned gene in pET system not being expressed - (reply: 10)
  20. Problem with subcloning genescript synthesized genes - (reply: 2)
  21. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
  22. Problem with double digest cloning - (reply: 3)
  23. How good is your cloning efficiency? - (reply: 2)
  24. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
  25. Cloning a 17 kb contruct - (reply: 2)
  26. Cloning of an unknown gene - (reply: 3)
  27. Cloning of an unknown gene - (reply: 3)
  28. cloning problems using in-fusion kit - (reply: 5)
  29. Cloning - Ligation problem - (reply: 3)
  30. How to clone a 2A peptide between two ORFs? - (reply: 2)
  31. TA Cloning - (reply: 11)
  32. Troubleshooting with cloning - (reply: 2)
  33. Suitable ratio of vector and insert in cloning - (reply: 5)
  34. Cloning a big insert in a small vector - (reply: 2)
  35. Cloning purified PCR product into cells - (reply: 5)
  36. Gateway cloning not working. - (reply: 7)
  37. Insert direction in TA cloning - (reply: 2)
  38. Problem with stable clone - (reply: 2)
  39. Should I proceed with clone selection? - (reply: 2)
  40. Can I loose the protein of interest from a stable clone? - (reply: 2)
  41. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
  42. Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
  43. Sticky and blunt end ligation in single reaction? - (reply: 2)
  44. How can we expand molecular cloning further? - (reply: 3)
  45. Molecular cloning using an ezh2 insert - (reply: 2)
  46. Subtle change in insert size after cloning - (reply: 6)
  47. lactic acid bacteria - cloning - (reply: 2)
  48. Can't isolate plasmid from cloned E.coli - (reply: 4)
  49. cloning issues - (reply: 3)
  50. Is important the direction of IRES-GFP in cloning? - (reply: 1)
  51. Cloning inserts: forward and reverse orientation - (reply: 4)
  52. What are the error-rates of the TA cloning Kits? - (reply: 8)
  53. What sorts of cloning strategies are available for creating inducible knockouts - (reply: 2)
  54. Can mutations be created during cloning? - (reply: 7)
  55. What is the full name for TA cloning - (reply: 4)
  56. cloning problem - (reply: 2)
  57. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
  58. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
  59. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
  60. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
  61. primers for cloning - (reply: 1)
  62. Cloning contamination - (reply: 3)
  63. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  64. Problem shrna cloning, bad sequencing results - (reply: 1)
  65. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  66. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
  67. Blunt end cloning - (reply: 12)
  68. Molecular cloning - (reply: 5)
  69. Nuclease free water for cloning - (reply: 8)
  70. Directional cloning 2bps overhang - (reply: 3)
  71. filling the digested vector and insert for blunt end cloning - (reply: 2)
  72. OVA cDNA clone? - (reply: 2)
  73. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
  74. Purpose of PolyA in cloning - (reply: 1)
  75. Cloning into pVIVO2 vector - (reply: 8)
  76. Cloning worked, but the expression vector is weird - (reply: 6)
  77. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
  78. Ubiquitin promoter cloning - (reply: 1)
  79. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  80. Is this counter-productive cloning or not? - (reply: 8)
  81. Failed cloning for 1month - TOPO - (reply: 7)
  82. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
  83. Which method for DNA purification b4 cloning - (reply: 4)
  84. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
  85. Failed TOPO cloning - what happened? - (reply: 9)
  86. TOPO cloning after Phusion-PCR - (reply: 1)
  87. Gene cloning with unknown sequence - (reply: 6)
  88. Failed cloning - different readings - (reply: 10)
  89. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
  90. Cloning pDsRed-Monomer-N1 - (reply: 1)
  91. Annexin V clone - (reply: 2)
  92. Please comment on my Cloning Plan - (reply: 1)
  93. Cloning problem still not solved - (reply: 2)
  94. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  95. cloning pEGFP-N1 - (reply: 1)
  96. Cloning beginner and clueless! - (reply: 1)
  97. Cloning Problems - (reply: 2)
  98. Different ways to ensure the successful cloning? - (reply: 2)
  99. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
  100. Sticky and blunt ligation issue - (reply: 6)
  101. cloning - (reply: 7)
  102. Help~ Incorrect constructs in cloning - (reply: 3)
  103. Blunt end is ligating to sticky end? - (reply: 2)
  104. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
  105. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
  106. Sticky to Blunt end (Hypothetical) - (reply: 2)
  107. Do genes always need to be cloned in the "right direction"? - (reply: 3)
  108. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  109. Cloning and expression problem - (reply: 5)
  110. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  111. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  112. cloning help; high salt level in vector - (reply: 4)
  113. problem with gateway cloning: missing part of insert - (reply: 3)
  114. Type of polymerases compatible for TA cloning - (reply: 7)
  115. Molecular Cloning Sambrook - (reply: 1)
  116. golden gate cloning - (reply: 3)
  117. types of dna cloning methods - (reply: 2)
  118. excision of fragment during cloning - (reply: 4)
  119. Unusual cloning issue... anyone else?? - (reply: 4)
  120. Sequencing of the cloned plasmid - (reply: 5)
  121. Cloning synthesised genes - (reply: 1)
  122. 4 way cloning into TA Vector - (reply: 10)
  123. Neeed Help , cloning Confirmation ?! - (reply: 5)
  124. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
  125. Newbie to smaI blunt end cloning! - (reply: 1)
  126. What part of the promoter to clone - (reply: 2)
  127. gene cloning, where to start? - (reply: 1)
  128. TA Cloning of ds cDNA - (reply: 6)
  129. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
  130. Cloning cDNA construct into E.Coli. - (reply: 4)
  131. Cloning large transgenes - (reply: 2)
  132. In-fusion HD cloning - (reply: 5)
  133. Few colonies frm sticky end cloning - (reply: 3)
  134. Problem with cloning - (reply: 7)
  135. Too many negative clones using Directional TOPO Cloning - (reply: 5)
  136. Designing cloning primers for DNMT - (reply: 2)
  137. Cloning advice - (reply: 3)
  138. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  139. Negative control antibodies (Anti-mouse C5 Clone BB5.1) - (reply: 1)
  140. Cloning large fragments - (reply: 9)
  141. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
  142. Cloning Mitochondrial Targeting Sequence - (reply: 4)
  143. problem in cloning - (reply: 2)
  144. TA cloning: ligation problem or toxic construct? - (reply: 4)
  145. Designing PCR Primers for cloning - (reply: 17)
  146. Including ATG in cloning? - (reply: 1)
  147. gene cloning - (reply: 1)
  148. Cloning repetitive sequences in mammalian cells - (reply: 3)
  149. Truncated gene Cloning - (reply: 1)
  150. Mutation in cloning - (reply: 6)
  151. Understand the basics of molecular cloning - (reply: 6)
  152. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
  153. what is the ORFeome clone? - (reply: 2)
  154. rescue cloning of a transposon mutant - (reply: 2)
  155. problem in cloning PCR primer design with restriction site - (reply: 4)
  156. Cloning at 6 kb gene - (reply: 3)
  157. Vector insert ratio in cloning - (reply: 3)
  158. polymerase to use for cloning - (reply: 4)
  159. Oligo insert cloning - (reply: 3)
  160. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  161. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  162. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
  163. Single RE site cloning - (reply: 3)
  164. Bisulfite PCR and cloning - (reply: 5)
  165. Cloning not working, all steps checked, clueless - (reply: 1)
  166. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
  167. Cloned but not expressing! - (reply: 8)
  168. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  169. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
  170. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
  171. TOPO TA CLONING - LIGATION STEP - (reply: 9)
  172. pEGFP C-1/N-1 Cloning - (reply: 11)
  173. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
  174. Problem with cloning - what is wrong? - (reply: 6)
  175. cloning: band at different site than desired after RE digestion - (reply: 11)
  176. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
  177. cloning pcr insert into plasmid - (reply: 2)
  178. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
  179. molecular cloning - (reply: 7)
  180. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  181. Another cloning hell....no transformants - (reply: 1)
  182. TOPO TA Cloning Troubleshooting - (reply: 1)
  183. TOPO TA Cloning - (reply: 1)
  184. No insert in subcloning - (reply: 3)
  185. Unable to clone in E. coli cells - (reply: 3)
  186. cloning into pPICZalphaA fails - (reply: 4)
  187. molecular cloning failure - (reply: 2)
  188. cloning antisense promoter in pGL3Basic - (reply: 2)
  189. Help - Troubleshooting cloning into pET28 - all screens positive, but sequencing - (reply: 2)
  190. Explanation of "In-Frame" cloning? - (reply: 6)
  191. Cloning Problem! I need help - (reply: 3)
  192. Help designing primers for use in In-fushion cloning - (reply: 4)
  193. Repeat of desired mutation in cloning product - (reply: 1)
  194. cloning a gene in to a plasmid - (reply: 5)
  195. recognition sites and cloning? - (reply: 2)
  196. question about PCR and cloning - (reply: 1)
  197. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  198. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
  199. Making a qPCR standard amplicon from cloning, why? - (reply: 4)
  200. help ,blunt end ligation - (reply: 4)
  201. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
  202. Subcloning - RE digest and ligation and transformation - (reply: 5)
  203. Making DNA Linkers for cloning purposes - (reply: 1)
  204. cloning short genomic DNA fragments - (reply: 1)
  205. How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
  206. cloning to pET28a - (reply: 1)
  207. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  208. CDS CLONING QUERY - (reply: 5)
  209. unable to amplify an infectious retrovirus clone - (reply: 4)
  210. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
  211. Cloning partial CDS - (reply: 5)
  212. Cloning problem into pGEMT-easy - (reply: 2)
  213. Cloning problem! - (reply: 8)
  214. Plasmid clone in BSP - (reply: 1)
  215. molecular cloning - (reply: 3)
  216. cloning without phosphorylation - (reply: 7)
  217. Cloning genetic sequences of proteins. - (reply: 1)
  218. should I do blunt end my pcr product before ligation - (reply: 2)
  219. Help with CGL gene cloning problems - (reply: 1)
  220. PUAST sub - cloning problem - (reply: 3)
  221. Cloning Problem - Ligation or Transformation - (reply: 4)
  222. Bisulfite cloning... reading material - (reply: 1)
  223. cloning into Pgem t easy - (reply: 1)
  224. I can´t obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
  225. cloning of a long gene using cDNA - (reply: 1)
  226. Isolation of a Gene of Interest for Subcloning - (reply: 8)
  227. Cloning into inducible vector but no expression - (reply: 1)
  228. weird problem in cloning!! - (reply: 4)
  229. Cloning HELP- Possible Primer issues - (reply: 6)
  230. Strain requirements for routine M13 phagemid cloning? - (reply: 1)
  231. Cloning in pETDuet-1 - (reply: 3)
  232. PCR cloning...help! - (reply: 5)
  233. Problems with blunt end ligation - (reply: 3)
  234. cloning problem - (reply: 2)
  235. problem in cloning involving partial digestion - (reply: 1)
  236. Design primers for expression cloning - (reply: 11)
  237. which software to draw the scheme of cloning strategy - (reply: 5)
  238. Blunt end ligation insert:vector ratio - (reply: 1)
  239. Has anyone ever had a large insert when subcloning? Mine is 4.3kb? - (reply: 2)
  240. Impact of primer sequence on TA cloning - (reply: 3)
  241. cloning an AT rich gene - (reply: 3)
  242. Cloning two genes in the same plasmid - (reply: 4)
  243. Problem: Cloning DNA shuffled band - (reply: 2)
  244. Transformation /Cloning Problem - (reply: 3)
  245. a robust multi-gene cloning method - (reply: 2)
  246. strange subcloning problem - (reply: 9)
  247. Cloned sequence is not predicted size by western blot - (reply: 1)
  248. good tutorials/protocols/books on gene cloning - (reply: 2)
  249. Cloning problem using pET32a vector - (reply: 3)
  250. Fixing SNPs after cloning - (reply: 5)
  251. emty vector cloning - (reply: 3)
  252. Double digest after cloning doesn't give gene - (reply: 6)
  253. Blunt end PCR product ligation - (reply: 1)
  254. strange cloning problem - (reply: 9)
  255. need help with subcloning !! - (reply: 2)
  256. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
  257. cloning with nested pcr - (reply: 3)
  258. cloning blues! - (reply: 12)
  259. Gateway cloning vector compatibility - (reply: 2)
  260. Cloning of IL8-promoter - (reply: 3)
  261. Cloning promoter (overlap with another gene!) - (reply: 3)
  262. Cloning a promoter - (reply: 2)
  263. Cloning two fragments into a vector - (reply: 3)
  264. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
  265. Problem with Blunt end ligation - (reply: 4)
  266. Cloning from a pGEMT Easy vector - (reply: 3)
  267. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  268. cloning problem - (reply: 9)
  269. Cloning genes into plant vector - (reply: 1)
  270. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
  271. Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
  272. Cloning App Released from Life Technologies - (reply: 2)
  273. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
  274. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  275. Blunt end cloning of 4Kb product - (reply: 3)
  276. competentce cell, cloning - (reply: 2)
  277. sequential cloning of multiple PCR products - how to do? (reply: 3)
  278. Cloning large gene - (reply: 3)
  279. Glycosilated peptide's cDNA cloning - (reply: 3)
  280. pWM91 cloning problem - (reply: 4)
  281. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
  282. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  283. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
  284. Cloning into lentiviral vector - (reply: 11)
  285. cloning failure help plzzz - cloning (reply: 1)
  286. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  287. PCR cloning problem - could not get colony (reply: 4)
  288. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  289. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  290. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  291. Cloning and expressing a mystery 71KDa protein - (reply: 9)
  292. Insert magically shortening??? - Cloning issues (reply: 2)
  293. Subcloning Problem - (reply: 3)
  294. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  295. cloning problem... - (reply: 5)
  296. cDNA clone - (reply: 1)
  297. miRNA cloning - (reply: 2)
  298. PCR: Cloning Primers - (reply: 8)
  299. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  300. Cloning does not work - (reply: 4)
  301. Help needed with subcloning problem - (reply: 6)
  302. Mouse huntingtin clone - (reply: 1)
  303. BLUNT CLONING With EcoNI - (reply: 1)
  304. Designing a multiple cloning site - (reply: 5)
  305. blunt and sticky end clonning temperature - (reply: 2)
  306. Most common mistakes - What mistakes are made during cloning? (reply: 7)
  307. Cloning direction / orientation? - (reply: 2)
  308. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
  309. optimal size for 3'UTR cloning - miRNA and 3'UTR cloning (reply: 2)
  310. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
  311. Cloning of ITS region - (reply: 6)
  312. SmaI blunt end ligation problem... - (reply: 3)
  313. Cell dilution cloning - Cells cloning (reply: 2)
  314. Why shouldn't you clone using expression bacteria - (reply: 6)
  315. metylated cloning vector - restriction digest of the insert (reply: 2)
  316. Secretion cloning vectors - (reply: 1)
  317. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
  318. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
  319. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
  320. cloning of race products - (reply: 1)
  321. Advice on amount of starting template for subcloning - (reply: 3)
  322. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
  323. cloning a 34kb insert - please suggest good vectors (reply: 1)
  324. Cloning issue - (reply: 3)
  325. Comment of this cloning strategy - (reply: 3)
  326. Cloning Problem: HSP90AA1 - Cloning Problem: HSP90AA1 (reply: 1)
  327. cloning a large insert - (reply: 5)
  328. Transposase contamination in cloning - (reply: 7)
  329. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  330. Cloning of a gene containing 31 exon - (reply: 1)
  331. cloning help - (reply: 3)
  332. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
  333. weird bands after minipreps - molecular cloning (reply: 2)
  334. purification of PCR product for cloning in a vector - (reply: 3)
  335. Cloning a foreign gene into E-coli - (reply: 1)
  336. subcloning into bacterial expression vector - (reply: 1)
  337. cloning into F1 ori of expression vector??? - (reply: 1)
  338. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  339. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  340. Cloning a Promoter - (reply: 3)
  341. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  342. glycerol stock preparation of recombinant clone - (reply: 2)
  343. Cloning - (reply: 1)
  344. Anyone make their own PCR cloning vector? - (reply: 5)
  345. Antibody gene cloning - (reply: 3)
  346. Which cloning kit should I use - (reply: 2)
  347. Topo cloning or pGEM-T easy ? - (reply: 3)
  348. Cloning the coding region of a protein - (reply: 4)
  349. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
  350. ta cloning problem - (reply: 2)
  351. Help: Cloning a 60KB fragment - (reply: 3)
  352. Cloning Problems/ Help - (reply: 2)
  353. clone GENE RT-PCT PRIMERS - cloning genes (reply: 1)
  354. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
  355. Molecular Cloning od Recombinant DNA - (reply: 3)
  356. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  357. obtain a full length cDNA clone WITH RT-PCR - Cloning (reply: 2)
  358. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  359. TOPO cloning problem - (reply: 4)
  360. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
  361. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  362. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  363. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
  364. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  365. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
  366. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
  367. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
  368. Cloning before sequencing- is it necessary??? - (reply: 13)
  369. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
  370. colony PCR for subcloning gene - (reply: 1)
  371. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  372. cloning of a 20 kb insert - (reply: 2)
  373. cloning of an operon - (reply: 2)
  374. Cloning problem - (reply: 2)
  375. Need advice on cloning very small insert into a vector - (reply: 3)
  376. A cloning nightmare - (reply: 8)
  377. Cloning problems i.e. Ligation - (reply: 2)
  378. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  379. cloning problem - (reply: 5)
  380. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  381. Blunt end filling - Blunt end filling (reply: 1)
  382. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
  383. TOPO Cloning - (reply: 5)
  384. blunt ligation - (reply: 4)
  385. DNA Sequencing / Cloning ? - (reply: 5)
  386. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  387. Cloning <100 bp fragments into a vector - (reply: 7)
  388. Cloning <100 bp fragments into a plasmid - (reply: 7)
  389. HpaI blunt end cloning - (reply: 1)
  390. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
  391. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
  392. Cloning in pCR2.1 plasmid - (reply: 7)
  393. Cloning a 3.5Kb fragment for sequencing - P-gem T-easy. (reply: 2)
  394. Advice on Cloning Strategy - (reply: 4)
  395. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
  396. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
  397. Ligation of Blunt PCR Product - (reply: 1)
  398. Cloning Help!! - It is getting very annoying... (reply: 13)
  399. sequence errors created during cloning - (reply: 6)
  400. PCR & Cloning - (reply: 6)
  401. Help cloning large insert into large vector - (reply: 7)
  402. blunt end ligation problem - Self ligation and truncate (reply: 1)
  403. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  404. Cloning: Randomly recombined Plasmids? - (reply: 6)
  405. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
  406. Cloning problems - (reply: 7)
  407. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  408. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  409. Blunt end ligation - Cant get ligation to work (reply: 4)
  410. blunt ligation - (reply: 1)
  411. please help!!cloning problems.. - (reply: 4)
  412. Cloning - PCR and Cloning problem (reply: 3)
  413. problem with cloning of dsred plz help - vector cut seems fine, still religation oocurs (reply: 13)
  414. cloning of GroEL - (reply: 3)
  415. Problems with High Background with Gateway Cloning - (reply: 1)
  416. blunt end ligation - how to solve the blunt end ligation?? (reply: 5)
  417. Removal of 5´ overhangs to form blunt ends - (reply: 1)
  418. problem with pcr cloning from mouse cDNA - (reply: 2)
  419. QUESTION about cloning - (reply: 5)
  420. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
  421. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  422. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  423. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  424. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  425. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
  426. TOPO TA cloning advice - (reply: 4)
  427. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  428. I have cloning problems with pGEM-T Easy - (reply: 5)
  429. blunt end cloning - (reply: 4)
  430. How can multiple cloning sites (MCS) NOT disrupt lacZ gene? - (reply: 2)
  431. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  432. How to retrieve precursor miRNA sequence with extra bases for cloning - (reply: 5)
  433. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  434. blunt-end / 3´A overhang cloning - (reply: 1)
  435. bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
  436. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  437. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  438. cloning - (reply: 6)
  439. Can't seem to do a simple cloning - (reply: 2)
  440. Cloning into pIBV5-His (not working) - (reply: 1)
  441. Cloning from cDNA - the wrong gene?! - (reply: 2)
  442. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
  443. circular or linear pDNA for stable clone generation? - (reply: 1)
  444. TOPO TA cloning problem - (reply: 12)
  445. cloning problem - (reply: 2)
  446. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
  447. serious problem in cloning - (reply: 2)
  448. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  449. Cloning using BamH1 and HindIII - (reply: 8)
  450. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
  451. TA cloning necessary? - (reply: 2)
  452. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  453. cloning - how to clone a gene having stop codons (reply: 4)
  454. Cloning advice needed - (reply: 4)
  455. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  456. subcloning problem - inframe fusion (reply: 1)
  457. ta cloning of pfu amplified fragment - (reply: 9)
  458. Double digestion and cloning - (reply: 2)
  459. cloning of long fragment - (reply: 1)
  460. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
  461. cloning of cellulose synthase gene - (reply: 1)
  462. problem in ligation and cloning - (reply: 1)
  463. cloning of small double strande DNA - I NEED HELP (reply: 3)
  464. Please Help: human Sox2, Oct 4 promoter region cloning - (reply: 2)
  465. concentration for cloning - (reply: 1)
  466. cDNA clone - (reply: 1)
  467. cloning of non-coding region for protein expression - protein expression (reply: 1)
  468. Insert Sequence Problem in Cloning - (reply: 2)
  469. insert sequence in clone - (reply: 6)
  470. Help: incompatible RE site subcloning - (reply: 1)
  471. need help cloning cdna of a known protein - (reply: 1)
  472. cloning in pET 30 B - (reply: 1)
  473. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
  474. Trouble with subcloning - (reply: 6)
  475. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
  476. Cloning Problem - (reply: 3)
  477. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
  478. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  479. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  480. Promoter Cloning for Luciferase Assay - (reply: 3)
  481. having the wrong vector in my clone - (reply: 3)
  482. cloning protocol - (reply: 1)
  483. Problem cloning bisulfite PCR BSP product - (reply: 6)
  484. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  485. problem with my clone - (reply: 3)
  486. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
  487. cloning in Bacteroides - (reply: 9)
  488. Cloning woes - Running out of ideas! - (reply: 7)
  489. Insert problems with TA cloning - (reply: 1)
  490. Unstable clone? - (reply: 1)
  491. Problems facing in cloning with pBI121 vector - (reply: 1)
  492. Cloning into pGem - Problems with 5kb insert (reply: 3)
  493. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  494. Free trial for BxSeqTools: Online Sequence Analysis and Cloning software - free trial for BxSeqTools: Online Sequence Analysis and Cloning (reply: 1)
  495. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  496. lentiviral system: cloning strategy - (reply: 2)
  497. Difficulties cloning by PCR - (reply: 5)
  498. problem with subcloning, please help! - (reply: 8)
  499. Analyse mutant clone - (reply: 7)
  500. Cloning by multiple oligo ligation - (reply: 2)
  501. anyone working currently on gateway cloning???? - (reply: 1)
  502. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  503. Cheap cloning options - any ideas????? (reply: 2)
  504. Subcloning - (reply: 3)
  505. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  506. Subcloning into pMIR-REPORT-luc - (reply: 7)
  507. Cloning big fragment into smaller vector - (reply: 3)
  508. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  509. Difficulty cloning vector with multiple Lox sites - (reply: 2)
  510. Transcription of double blunt end Clone - (reply: 2)
  511. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  512. Gateway cloning - (reply: 8)
  513. T A cloning problems - (reply: 3)
  514. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  515. Blunt-end cloning problem - (reply: 6)
  516. problem in gene cloning - (reply: 4)
  517. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  518. can't make the cloning work - (reply: 3)
  519. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
  520. cloning help - (reply: 9)
  521. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  522. gateway cloning - (reply: 3)
  523. Purifying and cloning 69nt product - (reply: 5)
  524. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
  525. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
  526. A gateway cloning expression vector question - (reply: 1)
  527. Promoter/gene cloning question - (reply: 7)
  528. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  529. insert is missing after TA cloning - TA cloning (reply: 6)
  530. cloning problem - molecular cloning (reply: 1)
  531. Cloning problems - 16Kb and 1.7Kb cloning problems (reply: 5)
  532. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
  533. wrong bands after T-vector cloning - (reply: 3)
  534. Help with cloning - (reply: 6)
  535. Help for T-A cloning - (reply: 15)
  536. No colony for oligo cloning - (reply: 9)
  537. blunt end ligation problem - (reply: 2)
  538. blunt ligation - (reply: 3)
  539. pENTR TOPO cloning - (reply: 1)
  540. yet one more cloning problem - (reply: 7)
  541. cloning miRNA library - (reply: 4)
  542. olligo annealing for shRNA cloning - (reply: 20)
  543. cloning issue - (reply: 1)
  544. Cloning dilemma - (reply: 3)
  545. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  546. Cloning Problems! Need Help! - (reply: 3)
  547. in frame problem after 3 months of cloning! - can I stil use my construct (reply: 5)
  548. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  549. cloning problem - (reply: 3)
  550. Cloning Conundrum - (reply: 7)
  551. TOPO cloning problem - (reply: 15)
  552. Please Help with Lentiviral Cloning - (reply: 1)
  553. Help with cloning!. - Cloning problems (reply: 20)
  554. Cloning PCR fragment - (reply: 1)
  555. molecular cloning - (reply: 2)
  556. DNA cloning - (reply: 4)
  557. Who can explain this? Weird cloning problem.... - (reply: 6)
  558. Help cloning!!! - As usual cloning is not working! (reply: 11)
  559. pSuper cloning problems - (reply: 1)
  560. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
  561. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  562. Receptor discovery/cloning - (reply: 1)
  563. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
  564. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  565. annoying PCR cloning problem (season 2) - (reply: 5)
  566. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  567. an annoying cloning - (reply: 9)
  568. Controls for TOPO cloning? - (reply: 1)
  569. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
  570. Problems cloning Hairpin vector - (reply: 3)
  571. different digestion pattern for same clone - (reply: 4)
  572. cloning trouble - (reply: 15)
  573. cDNA cloning problem - (reply: 1)
  574. How Gateway cloning system works? - (reply: 1)
  575. ds-oligo cloning/ligation issues - (reply: 18)
  576. Cloning multiple miR30 based shRNAs into a Lenti - (reply: 2)
  577. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  578. Help with cloning a plasmid that contains repeats! - (reply: 2)
  579. questions about TOPO cloning - (reply: 7)
  580. Subcloning into pBluescript - (reply: 6)
  581. How to control cloned cDNA expression level in cells? - (reply: 8)
  582. Cloning a labeled PCR product - (reply: 1)
  583. PCR and cloning - (reply: 14)
  584. Cloning trouble - (reply: 5)
  585. Problem cloning oligo into double digested vector - (reply: 9)
  586. cloning 1052bp fragment - no colonies (reply: 11)
  587. cloning problems - empty vector (reply: 3)
  588. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  589. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)