Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Cloning Related Discussions
  1. Designing Crispr guides and cloning into appropriate vectors - (reply: 1)
  2. Cloning protein of interest into lentiviral vector - help - I have no experience - (reply: 20)
  3. Trouble with PCR after cloning - (reply: 1)
  4. Troubleshooting with restriction enzymes during cloning - (reply: 5)
  5. Favorite polymerase for general cloning? - (reply: 3)
  6. restriction enzyme pcr cloning - (reply: 1)
  7. polb cloned in pET not expressed - (reply: 2)
  8. Restriction Digest with pGEMT for TA cloning - (reply: 19)
  9. Cloning of big inserts - (reply: 2)
  10. TA cloning..Urgent Help!! - (reply: 4)
  11. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
  12. Subcloning issue - (reply: 2)
  13. pGEM T Easy subcloning - (reply: 1)
  14. Target gene cloning - (reply: 4)
  15. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
  16. Problem with possible leaky expression in cloning - (reply: 4)
  17. Luciferase assay cloning - (reply: 2)
  18. Problem with restriction digestion of cloned PCR product - (reply: 5)
  19. SLIC Cloning Failure - (reply: 2)
  20. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  21. Polymerase mixture for blunt ended fragments - (reply: 2)
  22. Cloned gene in pET system not being expressed - (reply: 10)
  23. Problem with subcloning genescript synthesized genes - (reply: 2)
  24. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
  25. Problem with double digest cloning - (reply: 3)
  26. How good is your cloning efficiency? - (reply: 2)
  27. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
  28. Cloning a 17 kb contruct - (reply: 2)
  29. Cloning of an unknown gene - (reply: 4)
  30. Cloning of an unknown gene - (reply: 3)
  31. cloning problems using in-fusion kit - (reply: 5)
  32. Cloning - Ligation problem - (reply: 3)
  33. How to clone a 2A peptide between two ORFs? - (reply: 2)
  34. TA Cloning - (reply: 11)
  35. Troubleshooting with cloning - (reply: 2)
  36. Suitable ratio of vector and insert in cloning - (reply: 5)
  37. Cloning a big insert in a small vector - (reply: 2)
  38. Cloning purified PCR product into cells - (reply: 5)
  39. Gateway cloning not working. - (reply: 7)
  40. Insert direction in TA cloning - (reply: 2)
  41. Problem with stable clone - (reply: 2)
  42. Should I proceed with clone selection? - (reply: 2)
  43. Can I loose the protein of interest from a stable clone? - (reply: 2)
  44. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
  45. Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
  46. Sticky and blunt end ligation in single reaction? - (reply: 2)
  47. How can we expand molecular cloning further? - (reply: 3)
  48. Molecular cloning using an ezh2 insert - (reply: 2)
  49. Subtle change in insert size after cloning - (reply: 6)
  50. lactic acid bacteria - cloning - (reply: 2)
  51. Can't isolate plasmid from cloned E.coli - (reply: 4)
  52. cloning issues - (reply: 3)
  53. Is important the direction of IRES-GFP in cloning? - (reply: 1)
  54. Cloning inserts: forward and reverse orientation - (reply: 4)
  55. What are the error-rates of the TA cloning Kits? - (reply: 8)
  56. What sorts of cloning strategies are available for creating inducible knockouts - (reply: 2)
  57. Can mutations be created during cloning? - (reply: 7)
  58. What is the full name for TA cloning - (reply: 4)
  59. cloning problem - (reply: 2)
  60. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
  61. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
  62. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
  63. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
  64. primers for cloning - (reply: 1)
  65. Cloning contamination - (reply: 3)
  66. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  67. Problem shrna cloning, bad sequencing results - (reply: 1)
  68. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  69. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
  70. Blunt end cloning - (reply: 12)
  71. Molecular cloning - (reply: 5)
  72. Nuclease free water for cloning - (reply: 8)
  73. Directional cloning 2bps overhang - (reply: 3)
  74. filling the digested vector and insert for blunt end cloning - (reply: 2)
  75. OVA cDNA clone? - (reply: 2)
  76. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
  77. Purpose of PolyA in cloning - (reply: 1)
  78. Cloning into pVIVO2 vector - (reply: 8)
  79. Cloning worked, but the expression vector is weird - (reply: 6)
  80. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
  81. Ubiquitin promoter cloning - (reply: 1)
  82. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  83. Is this counter-productive cloning or not? - (reply: 8)
  84. Failed cloning for 1month - TOPO - (reply: 7)
  85. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
  86. Which method for DNA purification b4 cloning - (reply: 4)
  87. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
  88. Failed TOPO cloning - what happened? - (reply: 9)
  89. TOPO cloning after Phusion-PCR - (reply: 1)
  90. Gene cloning with unknown sequence - (reply: 6)
  91. Failed cloning - different readings - (reply: 10)
  92. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
  93. Cloning pDsRed-Monomer-N1 - (reply: 1)
  94. Annexin V clone - (reply: 2)
  95. Please comment on my Cloning Plan - (reply: 1)
  96. Cloning problem still not solved - (reply: 2)
  97. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  98. cloning pEGFP-N1 - (reply: 1)
  99. Cloning beginner and clueless! - (reply: 1)
  100. Cloning Problems - (reply: 2)
  101. Different ways to ensure the successful cloning? - (reply: 2)
  102. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
  103. Sticky and blunt ligation issue - (reply: 6)
  104. cloning - (reply: 7)
  105. Help~ Incorrect constructs in cloning - (reply: 3)
  106. Blunt end is ligating to sticky end? - (reply: 2)
  107. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
  108. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
  109. Sticky to Blunt end (Hypothetical) - (reply: 2)
  110. Do genes always need to be cloned in the "right direction"? - (reply: 3)
  111. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  112. Cloning and expression problem - (reply: 5)
  113. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  114. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  115. cloning help; high salt level in vector - (reply: 4)
  116. problem with gateway cloning: missing part of insert - (reply: 3)
  117. Type of polymerases compatible for TA cloning - (reply: 7)
  118. Molecular Cloning Sambrook - (reply: 1)
  119. golden gate cloning - (reply: 3)
  120. types of dna cloning methods - (reply: 2)
  121. excision of fragment during cloning - (reply: 4)
  122. Unusual cloning issue... anyone else?? - (reply: 4)
  123. Sequencing of the cloned plasmid - (reply: 5)
  124. Cloning synthesised genes - (reply: 1)
  125. 4 way cloning into TA Vector - (reply: 10)
  126. Neeed Help , cloning Confirmation ?! - (reply: 5)
  127. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
  128. Newbie to smaI blunt end cloning! - (reply: 1)
  129. What part of the promoter to clone - (reply: 2)
  130. gene cloning, where to start? - (reply: 1)
  131. TA Cloning of ds cDNA - (reply: 6)
  132. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
  133. Cloning cDNA construct into E.Coli. - (reply: 4)
  134. Cloning large transgenes - (reply: 2)
  135. In-fusion HD cloning - (reply: 5)
  136. Few colonies frm sticky end cloning - (reply: 3)
  137. Problem with cloning - (reply: 7)
  138. Too many negative clones using Directional TOPO Cloning - (reply: 5)
  139. Designing cloning primers for DNMT - (reply: 2)
  140. Cloning advice - (reply: 3)
  141. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  142. Negative control antibodies (Anti-mouse C5 Clone BB5.1) - (reply: 1)
  143. Cloning large fragments - (reply: 9)
  144. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
  145. Cloning Mitochondrial Targeting Sequence - (reply: 4)
  146. problem in cloning - (reply: 2)
  147. TA cloning: ligation problem or toxic construct? - (reply: 4)
  148. Designing PCR Primers for cloning - (reply: 17)
  149. Including ATG in cloning? - (reply: 1)
  150. gene cloning - (reply: 1)
  151. Cloning repetitive sequences in mammalian cells - (reply: 3)
  152. Truncated gene Cloning - (reply: 1)
  153. Mutation in cloning - (reply: 6)
  154. Understand the basics of molecular cloning - (reply: 6)
  155. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
  156. what is the ORFeome clone? - (reply: 2)
  157. rescue cloning of a transposon mutant - (reply: 2)
  158. problem in cloning PCR primer design with restriction site - (reply: 4)
  159. Cloning at 6 kb gene - (reply: 3)
  160. Vector insert ratio in cloning - (reply: 3)
  161. polymerase to use for cloning - (reply: 4)
  162. Oligo insert cloning - (reply: 3)
  163. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  164. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  165. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
  166. Single RE site cloning - (reply: 3)
  167. Bisulfite PCR and cloning - (reply: 5)
  168. Cloning not working, all steps checked, clueless - (reply: 1)
  169. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
  170. Cloned but not expressing! - (reply: 8)
  171. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  172. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
  173. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
  174. TOPO TA CLONING - LIGATION STEP - (reply: 9)
  175. pEGFP C-1/N-1 Cloning - (reply: 11)
  176. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
  177. Problem with cloning - what is wrong? - (reply: 6)
  178. cloning: band at different site than desired after RE digestion - (reply: 11)
  179. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
  180. cloning pcr insert into plasmid - (reply: 2)
  181. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
  182. molecular cloning - (reply: 7)
  183. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  184. Another cloning hell....no transformants - (reply: 1)
  185. TOPO TA Cloning Troubleshooting - (reply: 1)
  186. TOPO TA Cloning - (reply: 1)
  187. No insert in subcloning - (reply: 3)
  188. Unable to clone in E. coli cells - (reply: 3)
  189. cloning into pPICZalphaA fails - (reply: 4)
  190. molecular cloning failure - (reply: 2)
  191. cloning antisense promoter in pGL3Basic - (reply: 2)
  192. Help - Troubleshooting cloning into pET28 - all screens positive, but sequencing - (reply: 2)
  193. Explanation of "In-Frame" cloning? - (reply: 6)
  194. Cloning Problem! I need help - (reply: 3)
  195. Help designing primers for use in In-fushion cloning - (reply: 4)
  196. Repeat of desired mutation in cloning product - (reply: 1)
  197. cloning a gene in to a plasmid - (reply: 5)
  198. recognition sites and cloning? - (reply: 2)
  199. question about PCR and cloning - (reply: 1)
  200. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  201. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
  202. Making a qPCR standard amplicon from cloning, why? - (reply: 4)
  203. help ,blunt end ligation - (reply: 4)
  204. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
  205. Subcloning - RE digest and ligation and transformation - (reply: 5)
  206. Making DNA Linkers for cloning purposes - (reply: 1)
  207. cloning short genomic DNA fragments - (reply: 1)
  208. How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
  209. cloning to pET28a - (reply: 1)
  210. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  211. CDS CLONING QUERY - (reply: 5)
  212. unable to amplify an infectious retrovirus clone - (reply: 4)
  213. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
  214. Cloning partial CDS - (reply: 5)
  215. Cloning problem into pGEMT-easy - (reply: 2)
  216. Cloning problem! - (reply: 8)
  217. Plasmid clone in BSP - (reply: 1)
  218. molecular cloning - (reply: 3)
  219. cloning without phosphorylation - (reply: 7)
  220. Cloning genetic sequences of proteins. - (reply: 1)
  221. should I do blunt end my pcr product before ligation - (reply: 2)
  222. Help with CGL gene cloning problems - (reply: 1)
  223. PUAST sub - cloning problem - (reply: 3)
  224. Cloning Problem - Ligation or Transformation - (reply: 4)
  225. Bisulfite cloning... reading material - (reply: 1)
  226. cloning into Pgem t easy - (reply: 1)
  227. I can´t obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
  228. cloning of a long gene using cDNA - (reply: 1)
  229. Isolation of a Gene of Interest for Subcloning - (reply: 8)
  230. Cloning into inducible vector but no expression - (reply: 1)
  231. weird problem in cloning!! - (reply: 4)
  232. Cloning HELP- Possible Primer issues - (reply: 6)
  233. Strain requirements for routine M13 phagemid cloning? - (reply: 1)
  234. Cloning in pETDuet-1 - (reply: 3)
  235. PCR cloning...help! - (reply: 5)
  236. Problems with blunt end ligation - (reply: 3)
  237. cloning problem - (reply: 2)
  238. problem in cloning involving partial digestion - (reply: 1)
  239. Design primers for expression cloning - (reply: 11)
  240. which software to draw the scheme of cloning strategy - (reply: 5)
  241. Blunt end ligation insert:vector ratio - (reply: 1)
  242. Has anyone ever had a large insert when subcloning? Mine is 4.3kb? - (reply: 2)
  243. Impact of primer sequence on TA cloning - (reply: 3)
  244. cloning an AT rich gene - (reply: 3)
  245. Cloning two genes in the same plasmid - (reply: 4)
  246. Problem: Cloning DNA shuffled band - (reply: 2)
  247. Transformation /Cloning Problem - (reply: 3)
  248. a robust multi-gene cloning method - (reply: 2)
  249. strange subcloning problem - (reply: 9)
  250. Cloned sequence is not predicted size by western blot - (reply: 1)
  251. good tutorials/protocols/books on gene cloning - (reply: 2)
  252. Cloning problem using pET32a vector - (reply: 3)
  253. Fixing SNPs after cloning - (reply: 5)
  254. emty vector cloning - (reply: 3)
  255. Double digest after cloning doesn't give gene - (reply: 6)
  256. Blunt end PCR product ligation - (reply: 1)
  257. strange cloning problem - (reply: 9)
  258. need help with subcloning !! - (reply: 2)
  259. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
  260. cloning with nested pcr - (reply: 3)
  261. cloning blues! - (reply: 12)
  262. Gateway cloning vector compatibility - (reply: 2)
  263. Cloning of IL8-promoter - (reply: 3)
  264. Cloning promoter (overlap with another gene!) - (reply: 3)
  265. Cloning a promoter - (reply: 2)
  266. Cloning two fragments into a vector - (reply: 3)
  267. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
  268. Problem with Blunt end ligation - (reply: 4)
  269. Cloning from a pGEMT Easy vector - (reply: 3)
  270. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  271. cloning problem - (reply: 9)
  272. Cloning genes into plant vector - (reply: 1)
  273. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
  274. Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
  275. Cloning App Released from Life Technologies - (reply: 2)
  276. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
  277. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  278. Blunt end cloning of 4Kb product - (reply: 3)
  279. competentce cell, cloning - (reply: 2)
  280. sequential cloning of multiple PCR products - how to do? (reply: 3)
  281. Cloning large gene - (reply: 3)
  282. Glycosilated peptide's cDNA cloning - (reply: 3)
  283. pWM91 cloning problem - (reply: 4)
  284. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
  285. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  286. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
  287. Cloning into lentiviral vector - (reply: 11)
  288. cloning failure help plzzz - cloning (reply: 1)
  289. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  290. PCR cloning problem - could not get colony (reply: 4)
  291. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  292. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  293. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  294. Cloning and expressing a mystery 71KDa protein - (reply: 9)
  295. Insert magically shortening??? - Cloning issues (reply: 2)
  296. Subcloning Problem - (reply: 3)
  297. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  298. cloning problem... - (reply: 5)
  299. cDNA clone - (reply: 1)
  300. miRNA cloning - (reply: 2)
  301. PCR: Cloning Primers - (reply: 8)
  302. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  303. Cloning does not work - (reply: 4)
  304. Help needed with subcloning problem - (reply: 6)
  305. Mouse huntingtin clone - (reply: 1)
  306. BLUNT CLONING With EcoNI - (reply: 1)
  307. Designing a multiple cloning site - (reply: 5)
  308. blunt and sticky end clonning temperature - (reply: 2)
  309. Most common mistakes - What mistakes are made during cloning? (reply: 7)
  310. Cloning direction / orientation? - (reply: 2)
  311. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
  312. optimal size for 3'UTR cloning - miRNA and 3'UTR cloning (reply: 2)
  313. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
  314. Cloning of ITS region - (reply: 6)
  315. SmaI blunt end ligation problem... - (reply: 3)
  316. Cell dilution cloning - Cells cloning (reply: 2)
  317. Why shouldn't you clone using expression bacteria - (reply: 6)
  318. metylated cloning vector - restriction digest of the insert (reply: 2)
  319. Secretion cloning vectors - (reply: 1)
  320. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
  321. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
  322. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
  323. cloning of race products - (reply: 1)
  324. Advice on amount of starting template for subcloning - (reply: 3)
  325. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
  326. cloning a 34kb insert - please suggest good vectors (reply: 1)
  327. Cloning issue - (reply: 3)
  328. Comment of this cloning strategy - (reply: 3)
  329. Cloning Problem: HSP90AA1 - Cloning Problem: HSP90AA1 (reply: 1)
  330. cloning a large insert - (reply: 5)
  331. Transposase contamination in cloning - (reply: 7)
  332. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  333. Cloning of a gene containing 31 exon - (reply: 1)
  334. cloning help - (reply: 3)
  335. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
  336. weird bands after minipreps - molecular cloning (reply: 2)
  337. purification of PCR product for cloning in a vector - (reply: 3)
  338. Cloning a foreign gene into E-coli - (reply: 1)
  339. subcloning into bacterial expression vector - (reply: 1)
  340. cloning into F1 ori of expression vector??? - (reply: 1)
  341. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  342. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  343. Cloning a Promoter - (reply: 3)
  344. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  345. glycerol stock preparation of recombinant clone - (reply: 2)
  346. Cloning - (reply: 1)
  347. Anyone make their own PCR cloning vector? - (reply: 5)
  348. Antibody gene cloning - (reply: 3)
  349. Which cloning kit should I use - (reply: 2)
  350. Topo cloning or pGEM-T easy ? - (reply: 3)
  351. Cloning the coding region of a protein - (reply: 4)
  352. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
  353. ta cloning problem - (reply: 2)
  354. Help: Cloning a 60KB fragment - (reply: 3)
  355. Cloning Problems/ Help - (reply: 2)
  356. clone GENE RT-PCT PRIMERS - cloning genes (reply: 1)
  357. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
  358. Molecular Cloning od Recombinant DNA - (reply: 3)
  359. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  360. obtain a full length cDNA clone WITH RT-PCR - Cloning (reply: 2)
  361. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  362. TOPO cloning problem - (reply: 4)
  363. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
  364. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  365. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  366. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
  367. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  368. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
  369. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
  370. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
  371. Cloning before sequencing- is it necessary??? - (reply: 14)
  372. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
  373. colony PCR for subcloning gene - (reply: 1)
  374. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  375. cloning of a 20 kb insert - (reply: 2)
  376. cloning of an operon - (reply: 2)
  377. Cloning problem - (reply: 2)
  378. Need advice on cloning very small insert into a vector - (reply: 3)
  379. A cloning nightmare - (reply: 8)
  380. Cloning problems i.e. Ligation - (reply: 2)
  381. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  382. cloning problem - (reply: 5)
  383. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  384. Blunt end filling - Blunt end filling (reply: 1)
  385. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
  386. TOPO Cloning - (reply: 5)
  387. blunt ligation - (reply: 4)
  388. DNA Sequencing / Cloning ? - (reply: 5)
  389. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  390. Cloning <100 bp fragments into a vector - (reply: 7)
  391. Cloning <100 bp fragments into a plasmid - (reply: 7)
  392. HpaI blunt end cloning - (reply: 1)
  393. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
  394. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
  395. Cloning in pCR2.1 plasmid - (reply: 7)
  396. Cloning a 3.5Kb fragment for sequencing - P-gem T-easy. (reply: 2)
  397. Advice on Cloning Strategy - (reply: 4)
  398. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
  399. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
  400. Ligation of Blunt PCR Product - (reply: 1)
  401. Cloning Help!! - It is getting very annoying... (reply: 13)
  402. sequence errors created during cloning - (reply: 6)
  403. PCR & Cloning - (reply: 6)
  404. Help cloning large insert into large vector - (reply: 7)
  405. blunt end ligation problem - Self ligation and truncate (reply: 1)
  406. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  407. Cloning: Randomly recombined Plasmids? - (reply: 6)
  408. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
  409. Cloning problems - (reply: 7)
  410. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  411. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  412. Blunt end ligation - Cant get ligation to work (reply: 4)
  413. blunt ligation - (reply: 1)
  414. please help!!cloning problems.. - (reply: 4)
  415. Cloning - PCR and Cloning problem (reply: 3)
  416. problem with cloning of dsred plz help - vector cut seems fine, still religation oocurs (reply: 13)
  417. cloning of GroEL - (reply: 3)
  418. Problems with High Background with Gateway Cloning - (reply: 1)
  419. blunt end ligation - how to solve the blunt end ligation?? (reply: 5)
  420. Removal of 5´ overhangs to form blunt ends - (reply: 1)
  421. problem with pcr cloning from mouse cDNA - (reply: 2)
  422. QUESTION about cloning - (reply: 5)
  423. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
  424. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  425. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  426. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  427. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  428. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
  429. TOPO TA cloning advice - (reply: 4)
  430. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  431. I have cloning problems with pGEM-T Easy - (reply: 5)
  432. blunt end cloning - (reply: 4)
  433. How can multiple cloning sites (MCS) NOT disrupt lacZ gene? - (reply: 2)
  434. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  435. How to retrieve precursor miRNA sequence with extra bases for cloning - (reply: 5)
  436. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  437. blunt-end / 3´A overhang cloning - (reply: 1)
  438. bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
  439. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  440. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  441. cloning - (reply: 6)
  442. Can't seem to do a simple cloning - (reply: 2)
  443. Cloning into pIBV5-His (not working) - (reply: 1)
  444. Cloning from cDNA - the wrong gene?! - (reply: 2)
  445. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
  446. circular or linear pDNA for stable clone generation? - (reply: 1)
  447. TOPO TA cloning problem - (reply: 12)
  448. cloning problem - (reply: 2)
  449. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
  450. serious problem in cloning - (reply: 2)
  451. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  452. Cloning using BamH1 and HindIII - (reply: 8)
  453. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
  454. TA cloning necessary? - (reply: 2)
  455. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  456. cloning - how to clone a gene having stop codons (reply: 4)
  457. Cloning advice needed - (reply: 4)
  458. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  459. subcloning problem - inframe fusion (reply: 1)
  460. ta cloning of pfu amplified fragment - (reply: 9)
  461. Double digestion and cloning - (reply: 2)
  462. cloning of long fragment - (reply: 1)
  463. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
  464. cloning of cellulose synthase gene - (reply: 1)
  465. problem in ligation and cloning - (reply: 1)
  466. cloning of small double strande DNA - I NEED HELP (reply: 3)
  467. Please Help: human Sox2, Oct 4 promoter region cloning - (reply: 2)
  468. concentration for cloning - (reply: 1)
  469. cDNA clone - (reply: 1)
  470. cloning of non-coding region for protein expression - protein expression (reply: 1)
  471. Insert Sequence Problem in Cloning - (reply: 2)
  472. insert sequence in clone - (reply: 6)
  473. Help: incompatible RE site subcloning - (reply: 1)
  474. need help cloning cdna of a known protein - (reply: 1)
  475. cloning in pET 30 B - (reply: 1)
  476. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
  477. Trouble with subcloning - (reply: 6)
  478. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
  479. Cloning Problem - (reply: 3)
  480. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
  481. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  482. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  483. Promoter Cloning for Luciferase Assay - (reply: 3)
  484. having the wrong vector in my clone - (reply: 3)
  485. cloning protocol - (reply: 1)
  486. Problem cloning bisulfite PCR BSP product - (reply: 6)
  487. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  488. problem with my clone - (reply: 3)
  489. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
  490. cloning in Bacteroides - (reply: 9)
  491. Cloning woes - Running out of ideas! - (reply: 7)
  492. Insert problems with TA cloning - (reply: 1)
  493. Unstable clone? - (reply: 1)
  494. Problems facing in cloning with pBI121 vector - (reply: 1)
  495. Cloning into pGem - Problems with 5kb insert (reply: 3)
  496. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  497. Free trial for BxSeqTools: Online Sequence Analysis and Cloning software - free trial for BxSeqTools: Online Sequence Analysis and Cloning (reply: 1)
  498. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  499. lentiviral system: cloning strategy - (reply: 2)
  500. Difficulties cloning by PCR - (reply: 5)
  501. problem with subcloning, please help! - (reply: 8)
  502. Analyse mutant clone - (reply: 7)
  503. Cloning by multiple oligo ligation - (reply: 2)
  504. anyone working currently on gateway cloning???? - (reply: 1)
  505. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  506. Cheap cloning options - any ideas????? (reply: 2)
  507. Subcloning - (reply: 3)
  508. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  509. Subcloning into pMIR-REPORT-luc - (reply: 7)
  510. Cloning big fragment into smaller vector - (reply: 3)
  511. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  512. Difficulty cloning vector with multiple Lox sites - (reply: 2)
  513. Transcription of double blunt end Clone - (reply: 2)
  514. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  515. Gateway cloning - (reply: 8)
  516. T A cloning problems - (reply: 3)
  517. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  518. Blunt-end cloning problem - (reply: 6)
  519. problem in gene cloning - (reply: 4)
  520. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  521. can't make the cloning work - (reply: 3)
  522. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
  523. cloning help - (reply: 9)
  524. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  525. gateway cloning - (reply: 3)
  526. Purifying and cloning 69nt product - (reply: 5)
  527. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
  528. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
  529. A gateway cloning expression vector question - (reply: 1)
  530. Promoter/gene cloning question - (reply: 7)
  531. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  532. insert is missing after TA cloning - TA cloning (reply: 6)
  533. cloning problem - molecular cloning (reply: 1)
  534. Cloning problems - 16Kb and 1.7Kb cloning problems (reply: 5)
  535. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
  536. wrong bands after T-vector cloning - (reply: 3)
  537. Help with cloning - (reply: 6)
  538. Help for T-A cloning - (reply: 15)
  539. No colony for oligo cloning - (reply: 9)
  540. blunt end ligation problem - (reply: 2)
  541. blunt ligation - (reply: 3)
  542. pENTR TOPO cloning - (reply: 1)
  543. yet one more cloning problem - (reply: 7)
  544. cloning miRNA library - (reply: 4)
  545. olligo annealing for shRNA cloning - (reply: 20)
  546. cloning issue - (reply: 1)
  547. Cloning dilemma - (reply: 3)
  548. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  549. Cloning Problems! Need Help! - (reply: 3)
  550. in frame problem after 3 months of cloning! - can I stil use my construct (reply: 5)
  551. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  552. cloning problem - (reply: 3)
  553. Cloning Conundrum - (reply: 7)
  554. TOPO cloning problem - (reply: 15)
  555. Please Help with Lentiviral Cloning - (reply: 1)
  556. Help with cloning!. - Cloning problems (reply: 20)
  557. Cloning PCR fragment - (reply: 1)
  558. molecular cloning - (reply: 2)
  559. DNA cloning - (reply: 4)
  560. Who can explain this? Weird cloning problem.... - (reply: 6)
  561. Help cloning!!! - As usual cloning is not working! (reply: 11)
  562. pSuper cloning problems - (reply: 1)
  563. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
  564. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  565. Receptor discovery/cloning - (reply: 1)
  566. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
  567. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  568. annoying PCR cloning problem (season 2) - (reply: 5)
  569. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  570. an annoying cloning - (reply: 9)
  571. Controls for TOPO cloning? - (reply: 1)
  572. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
  573. Problems cloning Hairpin vector - (reply: 3)
  574. different digestion pattern for same clone - (reply: 4)
  575. cloning trouble - (reply: 15)
  576. cDNA cloning problem - (reply: 1)
  577. How Gateway cloning system works? - (reply: 1)
  578. ds-oligo cloning/ligation issues - (reply: 18)
  579. Cloning multiple miR30 based shRNAs into a Lenti - (reply: 2)
  580. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  581. Help with cloning a plasmid that contains repeats! - (reply: 2)
  582. questions about TOPO cloning - (reply: 7)
  583. Subcloning into pBluescript - (reply: 6)
  584. How to control cloned cDNA expression level in cells? - (reply: 8)
  585. Cloning a labeled PCR product - (reply: 1)
  586. PCR and cloning - (reply: 14)
  587. Cloning trouble - (reply: 5)
  588. Problem cloning oligo into double digested vector - (reply: 9)
  589. cloning 1052bp fragment - no colonies (reply: 11)
  590. cloning problems - empty vector (reply: 3)
  591. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  592. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)