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Cloning Related Discussions
  1. Trouble with PCR after cloning - (reply: 1)
  2. Troubleshooting with restriction enzymes during cloning - (reply: 4)
  3. Favorite polymerase for general cloning? - (reply: 3)
  4. restriction enzyme pcr cloning - (reply: 1)
  5. polb cloned in pET not expressed - (reply: 2)
  6. Restriction Digest with pGEMT for TA cloning - (reply: 19)
  7. Cloning of big inserts - (reply: 2)
  8. TA cloning..Urgent Help!! - (reply: 4)
  9. Wrong or No fragment in Directional Cloning Vector - (reply: 8)
  10. Subcloning issue - (reply: 2)
  11. pGEM T Easy subcloning - (reply: 1)
  12. Target gene cloning - (reply: 4)
  13. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
  14. Problem with possible leaky expression in cloning - (reply: 4)
  15. Luciferase assay cloning - (reply: 2)
  16. Problem with restriction digestion of cloned PCR product - (reply: 5)
  17. SLIC Cloning Failure - (reply: 2)
  18. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  19. Polymerase mixture for blunt ended fragments - (reply: 2)
  20. Cloned gene in pET system not being expressed - (reply: 10)
  21. Problem with subcloning genescript synthesized genes - (reply: 2)
  22. Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
  23. Problem with double digest cloning - (reply: 3)
  24. How good is your cloning efficiency? - (reply: 2)
  25. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
  26. Cloning a 17 kb contruct - (reply: 2)
  27. Cloning of an unknown gene - (reply: 3)
  28. Cloning of an unknown gene - (reply: 3)
  29. cloning problems using in-fusion kit - (reply: 5)
  30. Cloning - Ligation problem - (reply: 3)
  31. How to clone a 2A peptide between two ORFs? - (reply: 2)
  32. TA Cloning - (reply: 11)
  33. Troubleshooting with cloning - (reply: 2)
  34. Suitable ratio of vector and insert in cloning - (reply: 5)
  35. Cloning a big insert in a small vector - (reply: 2)
  36. Cloning purified PCR product into cells - (reply: 5)
  37. Gateway cloning not working. - (reply: 7)
  38. Insert direction in TA cloning - (reply: 2)
  39. Problem with stable clone - (reply: 2)
  40. Should I proceed with clone selection? - (reply: 2)
  41. Can I loose the protein of interest from a stable clone? - (reply: 2)
  42. Cloning Woes: Dealing with Viral ITRs - (reply: 1)
  43. Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
  44. Sticky and blunt end ligation in single reaction? - (reply: 2)
  45. How can we expand molecular cloning further? - (reply: 3)
  46. Molecular cloning using an ezh2 insert - (reply: 2)
  47. Subtle change in insert size after cloning - (reply: 6)
  48. lactic acid bacteria - cloning - (reply: 2)
  49. Can't isolate plasmid from cloned E.coli - (reply: 4)
  50. cloning issues - (reply: 3)
  51. Is important the direction of IRES-GFP in cloning? - (reply: 1)
  52. Cloning inserts: forward and reverse orientation - (reply: 4)
  53. What are the error-rates of the TA cloning Kits? - (reply: 8)
  54. What sorts of cloning strategies are available for creating inducible knockouts - (reply: 2)
  55. Can mutations be created during cloning? - (reply: 7)
  56. What is the full name for TA cloning - (reply: 4)
  57. cloning problem - (reply: 2)
  58. Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
  59. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
  60. Cloning GFP into pCMV-Tag2B vector - (reply: 1)
  61. Cloning an antimicrobial peptide that may kill the host - (reply: 1)
  62. primers for cloning - (reply: 1)
  63. Cloning contamination - (reply: 3)
  64. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  65. Problem shrna cloning, bad sequencing results - (reply: 1)
  66. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  67. pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
  68. Blunt end cloning - (reply: 12)
  69. Molecular cloning - (reply: 5)
  70. Nuclease free water for cloning - (reply: 8)
  71. Directional cloning 2bps overhang - (reply: 3)
  72. filling the digested vector and insert for blunt end cloning - (reply: 2)
  73. OVA cDNA clone? - (reply: 2)
  74. HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
  75. Purpose of PolyA in cloning - (reply: 1)
  76. Cloning into pVIVO2 vector - (reply: 8)
  77. Cloning worked, but the expression vector is weird - (reply: 6)
  78. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
  79. Ubiquitin promoter cloning - (reply: 1)
  80. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  81. Is this counter-productive cloning or not? - (reply: 8)
  82. Failed cloning for 1month - TOPO - (reply: 7)
  83. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
  84. Which method for DNA purification b4 cloning - (reply: 4)
  85. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
  86. Failed TOPO cloning - what happened? - (reply: 9)
  87. TOPO cloning after Phusion-PCR - (reply: 1)
  88. Gene cloning with unknown sequence - (reply: 6)
  89. Failed cloning - different readings - (reply: 10)
  90. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
  91. Cloning pDsRed-Monomer-N1 - (reply: 1)
  92. Annexin V clone - (reply: 2)
  93. Please comment on my Cloning Plan - (reply: 1)
  94. Cloning problem still not solved - (reply: 2)
  95. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  96. cloning pEGFP-N1 - (reply: 1)
  97. Cloning beginner and clueless! - (reply: 1)
  98. Cloning Problems - (reply: 2)
  99. Different ways to ensure the successful cloning? - (reply: 2)
  100. pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
  101. Sticky and blunt ligation issue - (reply: 6)
  102. cloning - (reply: 7)
  103. Help~ Incorrect constructs in cloning - (reply: 3)
  104. Blunt end is ligating to sticky end? - (reply: 2)
  105. Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
  106. Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
  107. Sticky to Blunt end (Hypothetical) - (reply: 2)
  108. Do genes always need to be cloned in the "right direction"? - (reply: 3)
  109. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  110. Cloning and expression problem - (reply: 5)
  111. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  112. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  113. cloning help; high salt level in vector - (reply: 4)
  114. problem with gateway cloning: missing part of insert - (reply: 3)
  115. Type of polymerases compatible for TA cloning - (reply: 7)
  116. Molecular Cloning Sambrook - (reply: 1)
  117. golden gate cloning - (reply: 3)
  118. types of dna cloning methods - (reply: 2)
  119. excision of fragment during cloning - (reply: 4)
  120. Unusual cloning issue... anyone else?? - (reply: 4)
  121. Sequencing of the cloned plasmid - (reply: 5)
  122. Cloning synthesised genes - (reply: 1)
  123. 4 way cloning into TA Vector - (reply: 10)
  124. Neeed Help , cloning Confirmation ?! - (reply: 5)
  125. Puzzling Cloning Problem With a Specific Vector - (reply: 1)
  126. Newbie to smaI blunt end cloning! - (reply: 1)
  127. What part of the promoter to clone - (reply: 2)
  128. gene cloning, where to start? - (reply: 1)
  129. TA Cloning of ds cDNA - (reply: 6)
  130. Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
  131. Cloning cDNA construct into E.Coli. - (reply: 4)
  132. Cloning large transgenes - (reply: 2)
  133. In-fusion HD cloning - (reply: 5)
  134. Few colonies frm sticky end cloning - (reply: 3)
  135. Problem with cloning - (reply: 7)
  136. Too many negative clones using Directional TOPO Cloning - (reply: 5)
  137. Designing cloning primers for DNMT - (reply: 2)
  138. Cloning advice - (reply: 3)
  139. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  140. Negative control antibodies (Anti-mouse C5 Clone BB5.1) - (reply: 1)
  141. Cloning large fragments - (reply: 9)
  142. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
  143. Cloning Mitochondrial Targeting Sequence - (reply: 4)
  144. problem in cloning - (reply: 2)
  145. TA cloning: ligation problem or toxic construct? - (reply: 4)
  146. Designing PCR Primers for cloning - (reply: 17)
  147. Including ATG in cloning? - (reply: 1)
  148. gene cloning - (reply: 1)
  149. Cloning repetitive sequences in mammalian cells - (reply: 3)
  150. Truncated gene Cloning - (reply: 1)
  151. Mutation in cloning - (reply: 6)
  152. Understand the basics of molecular cloning - (reply: 6)
  153. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
  154. what is the ORFeome clone? - (reply: 2)
  155. rescue cloning of a transposon mutant - (reply: 2)
  156. problem in cloning PCR primer design with restriction site - (reply: 4)
  157. Cloning at 6 kb gene - (reply: 3)
  158. Vector insert ratio in cloning - (reply: 3)
  159. polymerase to use for cloning - (reply: 4)
  160. Oligo insert cloning - (reply: 3)
  161. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
  162. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  163. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
  164. Single RE site cloning - (reply: 3)
  165. Bisulfite PCR and cloning - (reply: 5)
  166. Cloning not working, all steps checked, clueless - (reply: 1)
  167. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
  168. Cloned but not expressing! - (reply: 8)
  169. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  170. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
  171. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
  172. TOPO TA CLONING - LIGATION STEP - (reply: 9)
  173. pEGFP C-1/N-1 Cloning - (reply: 11)
  174. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
  175. Problem with cloning - what is wrong? - (reply: 6)
  176. cloning: band at different site than desired after RE digestion - (reply: 11)
  177. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
  178. cloning pcr insert into plasmid - (reply: 2)
  179. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
  180. molecular cloning - (reply: 7)
  181. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  182. Another cloning hell....no transformants - (reply: 1)
  183. TOPO TA Cloning Troubleshooting - (reply: 1)
  184. TOPO TA Cloning - (reply: 1)
  185. No insert in subcloning - (reply: 3)
  186. Unable to clone in E. coli cells - (reply: 3)
  187. cloning into pPICZalphaA fails - (reply: 4)
  188. molecular cloning failure - (reply: 2)
  189. cloning antisense promoter in pGL3Basic - (reply: 2)
  190. Help - Troubleshooting cloning into pET28 - all screens positive, but sequencing - (reply: 2)
  191. Explanation of "In-Frame" cloning? - (reply: 6)
  192. Cloning Problem! I need help - (reply: 3)
  193. Help designing primers for use in In-fushion cloning - (reply: 4)
  194. Repeat of desired mutation in cloning product - (reply: 1)
  195. cloning a gene in to a plasmid - (reply: 5)
  196. recognition sites and cloning? - (reply: 2)
  197. question about PCR and cloning - (reply: 1)
  198. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  199. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
  200. Making a qPCR standard amplicon from cloning, why? - (reply: 4)
  201. help ,blunt end ligation - (reply: 4)
  202. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
  203. Subcloning - RE digest and ligation and transformation - (reply: 5)
  204. Making DNA Linkers for cloning purposes - (reply: 1)
  205. cloning short genomic DNA fragments - (reply: 1)
  206. How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
  207. cloning to pET28a - (reply: 1)
  208. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  209. CDS CLONING QUERY - (reply: 5)
  210. unable to amplify an infectious retrovirus clone - (reply: 4)
  211. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
  212. Cloning partial CDS - (reply: 5)
  213. Cloning problem into pGEMT-easy - (reply: 2)
  214. Cloning problem! - (reply: 8)
  215. Plasmid clone in BSP - (reply: 1)
  216. molecular cloning - (reply: 3)
  217. cloning without phosphorylation - (reply: 7)
  218. Cloning genetic sequences of proteins. - (reply: 1)
  219. should I do blunt end my pcr product before ligation - (reply: 2)
  220. Help with CGL gene cloning problems - (reply: 1)
  221. PUAST sub - cloning problem - (reply: 3)
  222. Cloning Problem - Ligation or Transformation - (reply: 4)
  223. Bisulfite cloning... reading material - (reply: 1)
  224. cloning into Pgem t easy - (reply: 1)
  225. I can´t obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
  226. cloning of a long gene using cDNA - (reply: 1)
  227. Isolation of a Gene of Interest for Subcloning - (reply: 8)
  228. Cloning into inducible vector but no expression - (reply: 1)
  229. weird problem in cloning!! - (reply: 4)
  230. Cloning HELP- Possible Primer issues - (reply: 6)
  231. Strain requirements for routine M13 phagemid cloning? - (reply: 1)
  232. Cloning in pETDuet-1 - (reply: 3)
  233. PCR cloning...help! - (reply: 5)
  234. Problems with blunt end ligation - (reply: 3)
  235. cloning problem - (reply: 2)
  236. problem in cloning involving partial digestion - (reply: 1)
  237. Design primers for expression cloning - (reply: 11)
  238. which software to draw the scheme of cloning strategy - (reply: 5)
  239. Blunt end ligation insert:vector ratio - (reply: 1)
  240. Has anyone ever had a large insert when subcloning? Mine is 4.3kb? - (reply: 2)
  241. Impact of primer sequence on TA cloning - (reply: 3)
  242. cloning an AT rich gene - (reply: 3)
  243. Cloning two genes in the same plasmid - (reply: 4)
  244. Problem: Cloning DNA shuffled band - (reply: 2)
  245. Transformation /Cloning Problem - (reply: 3)
  246. a robust multi-gene cloning method - (reply: 2)
  247. strange subcloning problem - (reply: 9)
  248. Cloned sequence is not predicted size by western blot - (reply: 1)
  249. good tutorials/protocols/books on gene cloning - (reply: 2)
  250. Cloning problem using pET32a vector - (reply: 3)
  251. Fixing SNPs after cloning - (reply: 5)
  252. emty vector cloning - (reply: 3)
  253. Double digest after cloning doesn't give gene - (reply: 6)
  254. Blunt end PCR product ligation - (reply: 1)
  255. strange cloning problem - (reply: 9)
  256. need help with subcloning !! - (reply: 2)
  257. How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
  258. cloning with nested pcr - (reply: 3)
  259. cloning blues! - (reply: 12)
  260. Gateway cloning vector compatibility - (reply: 2)
  261. Cloning of IL8-promoter - (reply: 3)
  262. Cloning promoter (overlap with another gene!) - (reply: 3)
  263. Cloning a promoter - (reply: 2)
  264. Cloning two fragments into a vector - (reply: 3)
  265. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
  266. Problem with Blunt end ligation - (reply: 4)
  267. Cloning from a pGEMT Easy vector - (reply: 3)
  268. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  269. cloning problem - (reply: 9)
  270. Cloning genes into plant vector - (reply: 1)
  271. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
  272. Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
  273. Cloning App Released from Life Technologies - (reply: 2)
  274. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
  275. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  276. Blunt end cloning of 4Kb product - (reply: 3)
  277. competentce cell, cloning - (reply: 2)
  278. sequential cloning of multiple PCR products - how to do? (reply: 3)
  279. Cloning large gene - (reply: 3)
  280. Glycosilated peptide's cDNA cloning - (reply: 3)
  281. pWM91 cloning problem - (reply: 4)
  282. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
  283. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  284. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
  285. Cloning into lentiviral vector - (reply: 11)
  286. cloning failure help plzzz - cloning (reply: 1)
  287. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  288. PCR cloning problem - could not get colony (reply: 4)
  289. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  290. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  291. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  292. Cloning and expressing a mystery 71KDa protein - (reply: 9)
  293. Insert magically shortening??? - Cloning issues (reply: 2)
  294. Subcloning Problem - (reply: 3)
  295. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
  296. cloning problem... - (reply: 5)
  297. cDNA clone - (reply: 1)
  298. miRNA cloning - (reply: 2)
  299. PCR: Cloning Primers - (reply: 8)
  300. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  301. Cloning does not work - (reply: 4)
  302. Help needed with subcloning problem - (reply: 6)
  303. Mouse huntingtin clone - (reply: 1)
  304. BLUNT CLONING With EcoNI - (reply: 1)
  305. Designing a multiple cloning site - (reply: 5)
  306. blunt and sticky end clonning temperature - (reply: 2)
  307. Most common mistakes - What mistakes are made during cloning? (reply: 7)
  308. Cloning direction / orientation? - (reply: 2)
  309. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
  310. optimal size for 3'UTR cloning - miRNA and 3'UTR cloning (reply: 2)
  311. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
  312. Cloning of ITS region - (reply: 6)
  313. SmaI blunt end ligation problem... - (reply: 3)
  314. Cell dilution cloning - Cells cloning (reply: 2)
  315. Why shouldn't you clone using expression bacteria - (reply: 6)
  316. metylated cloning vector - restriction digest of the insert (reply: 2)
  317. Secretion cloning vectors - (reply: 1)
  318. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
  319. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
  320. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
  321. cloning of race products - (reply: 1)
  322. Advice on amount of starting template for subcloning - (reply: 3)
  323. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
  324. cloning a 34kb insert - please suggest good vectors (reply: 1)
  325. Cloning issue - (reply: 3)
  326. Comment of this cloning strategy - (reply: 3)
  327. Cloning Problem: HSP90AA1 - Cloning Problem: HSP90AA1 (reply: 1)
  328. cloning a large insert - (reply: 5)
  329. Transposase contamination in cloning - (reply: 7)
  330. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  331. Cloning of a gene containing 31 exon - (reply: 1)
  332. cloning help - (reply: 3)
  333. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
  334. weird bands after minipreps - molecular cloning (reply: 2)
  335. purification of PCR product for cloning in a vector - (reply: 3)
  336. Cloning a foreign gene into E-coli - (reply: 1)
  337. subcloning into bacterial expression vector - (reply: 1)
  338. cloning into F1 ori of expression vector??? - (reply: 1)
  339. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  340. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  341. Cloning a Promoter - (reply: 3)
  342. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  343. glycerol stock preparation of recombinant clone - (reply: 2)
  344. Cloning - (reply: 1)
  345. Anyone make their own PCR cloning vector? - (reply: 5)
  346. Antibody gene cloning - (reply: 3)
  347. Which cloning kit should I use - (reply: 2)
  348. Topo cloning or pGEM-T easy ? - (reply: 3)
  349. Cloning the coding region of a protein - (reply: 4)
  350. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
  351. ta cloning problem - (reply: 2)
  352. Help: Cloning a 60KB fragment - (reply: 3)
  353. Cloning Problems/ Help - (reply: 2)
  354. clone GENE RT-PCT PRIMERS - cloning genes (reply: 1)
  355. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
  356. Molecular Cloning od Recombinant DNA - (reply: 3)
  357. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  358. obtain a full length cDNA clone WITH RT-PCR - Cloning (reply: 2)
  359. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  360. TOPO cloning problem - (reply: 4)
  361. Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
  362. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  363. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  364. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
  365. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  366. cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
  367. Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
  368. Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
  369. Cloning before sequencing- is it necessary??? - (reply: 13)
  370. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
  371. colony PCR for subcloning gene - (reply: 1)
  372. Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
  373. cloning of a 20 kb insert - (reply: 2)
  374. cloning of an operon - (reply: 2)
  375. Cloning problem - (reply: 2)
  376. Need advice on cloning very small insert into a vector - (reply: 3)
  377. A cloning nightmare - (reply: 8)
  378. Cloning problems i.e. Ligation - (reply: 2)
  379. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  380. cloning problem - (reply: 5)
  381. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
  382. Blunt end filling - Blunt end filling (reply: 1)
  383. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
  384. TOPO Cloning - (reply: 5)
  385. blunt ligation - (reply: 4)
  386. DNA Sequencing / Cloning ? - (reply: 5)
  387. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  388. Cloning <100 bp fragments into a vector - (reply: 7)
  389. Cloning <100 bp fragments into a plasmid - (reply: 7)
  390. HpaI blunt end cloning - (reply: 1)
  391. New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
  392. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
  393. Cloning in pCR2.1 plasmid - (reply: 7)
  394. Cloning a 3.5Kb fragment for sequencing - P-gem T-easy. (reply: 2)
  395. Advice on Cloning Strategy - (reply: 4)
  396. Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
  397. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
  398. Ligation of Blunt PCR Product - (reply: 1)
  399. Cloning Help!! - It is getting very annoying... (reply: 13)
  400. sequence errors created during cloning - (reply: 6)
  401. PCR & Cloning - (reply: 6)
  402. Help cloning large insert into large vector - (reply: 7)
  403. blunt end ligation problem - Self ligation and truncate (reply: 1)
  404. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  405. Cloning: Randomly recombined Plasmids? - (reply: 6)
  406. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
  407. Cloning problems - (reply: 7)
  408. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  409. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  410. Blunt end ligation - Cant get ligation to work (reply: 4)
  411. blunt ligation - (reply: 1)
  412. please help!!cloning problems.. - (reply: 4)
  413. Cloning - PCR and Cloning problem (reply: 3)
  414. problem with cloning of dsred plz help - vector cut seems fine, still religation oocurs (reply: 13)
  415. cloning of GroEL - (reply: 3)
  416. Problems with High Background with Gateway Cloning - (reply: 1)
  417. blunt end ligation - how to solve the blunt end ligation?? (reply: 5)
  418. Removal of 5´ overhangs to form blunt ends - (reply: 1)
  419. problem with pcr cloning from mouse cDNA - (reply: 2)
  420. QUESTION about cloning - (reply: 5)
  421. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
  422. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  423. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  424. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  425. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  426. CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
  427. TOPO TA cloning advice - (reply: 4)
  428. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  429. I have cloning problems with pGEM-T Easy - (reply: 5)
  430. blunt end cloning - (reply: 4)
  431. How can multiple cloning sites (MCS) NOT disrupt lacZ gene? - (reply: 2)
  432. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  433. How to retrieve precursor miRNA sequence with extra bases for cloning - (reply: 5)
  434. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  435. blunt-end / 3´A overhang cloning - (reply: 1)
  436. bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
  437. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  438. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  439. cloning - (reply: 6)
  440. Can't seem to do a simple cloning - (reply: 2)
  441. Cloning into pIBV5-His (not working) - (reply: 1)
  442. Cloning from cDNA - the wrong gene?! - (reply: 2)
  443. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
  444. circular or linear pDNA for stable clone generation? - (reply: 1)
  445. TOPO TA cloning problem - (reply: 12)
  446. cloning problem - (reply: 2)
  447. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
  448. serious problem in cloning - (reply: 2)
  449. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  450. Cloning using BamH1 and HindIII - (reply: 8)
  451. minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
  452. TA cloning necessary? - (reply: 2)
  453. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  454. cloning - how to clone a gene having stop codons (reply: 4)
  455. Cloning advice needed - (reply: 4)
  456. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  457. subcloning problem - inframe fusion (reply: 1)
  458. ta cloning of pfu amplified fragment - (reply: 9)
  459. Double digestion and cloning - (reply: 2)
  460. cloning of long fragment - (reply: 1)
  461. AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
  462. cloning of cellulose synthase gene - (reply: 1)
  463. problem in ligation and cloning - (reply: 1)
  464. cloning of small double strande DNA - I NEED HELP (reply: 3)
  465. Please Help: human Sox2, Oct 4 promoter region cloning - (reply: 2)
  466. concentration for cloning - (reply: 1)
  467. cDNA clone - (reply: 1)
  468. cloning of non-coding region for protein expression - protein expression (reply: 1)
  469. Insert Sequence Problem in Cloning - (reply: 2)
  470. insert sequence in clone - (reply: 6)
  471. Help: incompatible RE site subcloning - (reply: 1)
  472. need help cloning cdna of a known protein - (reply: 1)
  473. cloning in pET 30 B - (reply: 1)
  474. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
  475. Trouble with subcloning - (reply: 6)
  476. about pre-miRNA cloned into lentivirus vectors - (reply: 4)
  477. Cloning Problem - (reply: 3)
  478. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
  479. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  480. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  481. Promoter Cloning for Luciferase Assay - (reply: 3)
  482. having the wrong vector in my clone - (reply: 3)
  483. cloning protocol - (reply: 1)
  484. Problem cloning bisulfite PCR BSP product - (reply: 6)
  485. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
  486. problem with my clone - (reply: 3)
  487. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
  488. cloning in Bacteroides - (reply: 9)
  489. Cloning woes - Running out of ideas! - (reply: 7)
  490. Insert problems with TA cloning - (reply: 1)
  491. Unstable clone? - (reply: 1)
  492. Problems facing in cloning with pBI121 vector - (reply: 1)
  493. Cloning into pGem - Problems with 5kb insert (reply: 3)
  494. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  495. Free trial for BxSeqTools: Online Sequence Analysis and Cloning software - free trial for BxSeqTools: Online Sequence Analysis and Cloning (reply: 1)
  496. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  497. lentiviral system: cloning strategy - (reply: 2)
  498. Difficulties cloning by PCR - (reply: 5)
  499. problem with subcloning, please help! - (reply: 8)
  500. Analyse mutant clone - (reply: 7)
  501. Cloning by multiple oligo ligation - (reply: 2)
  502. anyone working currently on gateway cloning???? - (reply: 1)
  503. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  504. Cheap cloning options - any ideas????? (reply: 2)
  505. Subcloning - (reply: 3)
  506. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  507. Subcloning into pMIR-REPORT-luc - (reply: 7)
  508. Cloning big fragment into smaller vector - (reply: 3)
  509. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  510. Difficulty cloning vector with multiple Lox sites - (reply: 2)
  511. Transcription of double blunt end Clone - (reply: 2)
  512. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  513. Gateway cloning - (reply: 8)
  514. T A cloning problems - (reply: 3)
  515. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
  516. Blunt-end cloning problem - (reply: 6)
  517. problem in gene cloning - (reply: 4)
  518. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  519. can't make the cloning work - (reply: 3)
  520. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
  521. cloning help - (reply: 9)
  522. Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
  523. gateway cloning - (reply: 3)
  524. Purifying and cloning 69nt product - (reply: 5)
  525. Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
  526. InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
  527. A gateway cloning expression vector question - (reply: 1)
  528. Promoter/gene cloning question - (reply: 7)
  529. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  530. insert is missing after TA cloning - TA cloning (reply: 6)
  531. cloning problem - molecular cloning (reply: 1)
  532. Cloning problems - 16Kb and 1.7Kb cloning problems (reply: 5)
  533. Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
  534. wrong bands after T-vector cloning - (reply: 3)
  535. Help with cloning - (reply: 6)
  536. Help for T-A cloning - (reply: 15)
  537. No colony for oligo cloning - (reply: 9)
  538. blunt end ligation problem - (reply: 2)
  539. blunt ligation - (reply: 3)
  540. pENTR TOPO cloning - (reply: 1)
  541. yet one more cloning problem - (reply: 7)
  542. cloning miRNA library - (reply: 4)
  543. olligo annealing for shRNA cloning - (reply: 20)
  544. cloning issue - (reply: 1)
  545. Cloning dilemma - (reply: 3)
  546. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  547. Cloning Problems! Need Help! - (reply: 3)
  548. in frame problem after 3 months of cloning! - can I stil use my construct (reply: 5)
  549. Cloning done....sequence of the insert for the expression vector - (reply: 1)
  550. cloning problem - (reply: 3)
  551. Cloning Conundrum - (reply: 7)
  552. TOPO cloning problem - (reply: 15)
  553. Please Help with Lentiviral Cloning - (reply: 1)
  554. Help with cloning!. - Cloning problems (reply: 20)
  555. Cloning PCR fragment - (reply: 1)
  556. molecular cloning - (reply: 2)
  557. DNA cloning - (reply: 4)
  558. Who can explain this? Weird cloning problem.... - (reply: 6)
  559. Help cloning!!! - As usual cloning is not working! (reply: 11)
  560. pSuper cloning problems - (reply: 1)
  561. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
  562. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  563. Receptor discovery/cloning - (reply: 1)
  564. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
  565. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  566. annoying PCR cloning problem (season 2) - (reply: 5)
  567. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  568. an annoying cloning - (reply: 9)
  569. Controls for TOPO cloning? - (reply: 1)
  570. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
  571. Problems cloning Hairpin vector - (reply: 3)
  572. different digestion pattern for same clone - (reply: 4)
  573. cloning trouble - (reply: 15)
  574. cDNA cloning problem - (reply: 1)
  575. How Gateway cloning system works? - (reply: 1)
  576. ds-oligo cloning/ligation issues - (reply: 18)
  577. Cloning multiple miR30 based shRNAs into a Lenti - (reply: 2)
  578. vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
  579. Help with cloning a plasmid that contains repeats! - (reply: 2)
  580. questions about TOPO cloning - (reply: 7)
  581. Subcloning into pBluescript - (reply: 6)
  582. How to control cloned cDNA expression level in cells? - (reply: 8)
  583. Cloning a labeled PCR product - (reply: 1)
  584. PCR and cloning - (reply: 14)
  585. Cloning trouble - (reply: 5)
  586. Problem cloning oligo into double digested vector - (reply: 9)
  587. cloning 1052bp fragment - no colonies (reply: 11)
  588. cloning problems - empty vector (reply: 3)
  589. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  590. cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)