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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. LMW proteins not transfer of gel to membrane - (reply: 1)
182. smear in SDS PAGE - (reply: 4)
183. Insufficient Transfer of proteins through semi dry transfer - (reply: 6)
184. Smeared Bands after Ponceau Staining? - (reply: 3)
185. If preimmune sera and immune sera are both reacting to recombinat antigen in wes - (reply: 7)
186. patchy/holey bands after transfer, invasive bubbles? - (reply: 7)
187. Western not working - (reply: 4)
188. fused bands of beta-actin in western blot - (reply: 2)
189. Suggestions - Nice Western Blot Image from Office Scanner? - (reply: 7)
190. Undergrad "activated" a NS membrane with 100% MeOH... Any hope? - (reply: 5)
191. Problem with GFP detection in western - (reply: 6)
192. Sudden Trouble with PVDF Transfer - (reply: 6)
193. Equal Loading Confusion - (reply: 6)
194. Wrong protein size on western blot - (reply: 10)
195. How to know if the signal of western blot is saturated ? - (reply: 4)
196. Scotch-Brite versus other foam pad for wet transfer? - (reply: 3)
197. 5x SDS lysis buffer - (reply: 5)
198. Protein measurement of Laemmli samples - (reply: 2)
199. SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
200. Uneven banding - Western blot troubleshooting - (reply: 4)
201. Western Blot with fixed cells - (reply: 5)
202. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
203. Primary antibody - (reply: 1)
204. Native-PAGE, help! - (reply: 3)
205. Trouble with purified recombinant protein bands - (reply: 3)
206. Stain in electrophoresis is going from + to - - (reply: 3)
207. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
208. Extraction of Transmembrane Proteins - (reply: 1)
209. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
210. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)

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