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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. How to load equal amount of protein without using spectramax - (reply: 4)
182. Western blot membrane imaging - (reply: 7)
183. Detection of T7 RNA polymerase by WB - (reply: 6)
184. Shift in the position of band onto the western blot - (reply: 3)
185. Ghost and inverse bands and high background. Help! - (reply: 3)
186. No resolving of protein/bands below 50kDa :-s - (reply: 5)
187. Problems with wet transfer blot - (reply: 2)
188. 5% BSA blocking buffer - (reply: 3)
189. Storage of Membrane before addition of primary antibody - (reply: 3)
190. "Mickey Mouse" bands - (reply: 5)
191. SDS-PAGE : when the transfer stops - (reply: 1)
192. confusing Western results - (reply: 5)
193. aditional band on WB - (reply: 1)
194. Transferring >150 kDA proteins conditions - (reply: 3)
195. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
196. TAU gel electrophoresis of histones - (reply: 4)
197. Bands dark on sides and light in middle - (reply: 7)
198. Addition of sample loading buffer, before or after heating - (reply: 5)
199. question about protein expression normalization. - (reply: 6)
200. Protein bands not migrating - (reply: 3)
201. Help with high background (have tried most trouble shooting) - (reply: 8)
202. Tumor protein precipitation at denaturing step? - (reply: 1)
203. How to prepare blood serum for western blot? - (reply: 3)
204. TNF alpha and IL 1b Western Blot - (reply: 1)
205. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
206. SDS-PAGE trouble with protein concentration - (reply: 2)
207. Acrylamid - (reply: 5)
208. Detecting doublet bands in Western for eGFP control - (reply: 1)
209. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
210. Protein Size 50 kDa higher than expected - (reply: 3)

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