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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. 10x Laemmli buffer - (reply: 6)
182. Need help translating my western blot protocol into every language - (reply: 3)
183. Semi-dry system for NuPage with MES buffer - (reply: 4)
184. Recommend anti-rat HRP secondary antibody - (reply: 2)
185. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
186. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)
187. Why Squeezing of lanes occur at bottom in PAGE - (reply: 6)
188. Western blot to CD44? - (reply: 3)
189. Amount of EDTA - (reply: 4)
190. SDS Apparatus for northern - (reply: 1)
191. Prey protein band bleeds, IgG band normal? - (reply: 3)
192. Coomassie gel after semi-dry transfer - normal like this? - (reply: 7)
193. His-Probe - (reply: 2)
194. Problems with western membrane stripping - (reply: 2)
195. Can protein isomers be detected in SDS-PAGE - (reply: 3)
196. western blot of cardiomyocytes - (reply: 2)
197. Gradient Gel Electrophoresis - (reply: 3)
198. SDS-PAGE resolving gel not completely set - (reply: 4)
199. Problem with SDS-PAGE gel - (reply: 13)
200. How to dissolve TCA/acetone precipitated proteins? - (reply: 1)
201. SDS-Page trouble-shooting - (reply: 3)
202. PVDF membrane long-term storage after transfer - (reply: 3)
203. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
204. Distorted bands from human colon samples - (reply: 2)
205. "Wavy" bands in SDS-PAGE gel - (reply: 5)
206. LMW proteins not transfer of gel to membrane - (reply: 1)
207. smear in SDS PAGE - (reply: 4)
208. Insufficient Transfer of proteins through semi dry transfer - (reply: 6)
209. Smeared Bands after Ponceau Staining? - (reply: 3)
210. If preimmune sera and immune sera are both reacting to recombinat antigen in wes - (reply: 7)

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