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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. Western Blotting loading control for large proteins - (reply: 2)
182. Uneven beta actin - (reply: 2)
183. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
184. NCOR1 western - (reply: 8)
185. Discrepancy between WB and Flow cytometry result - (reply: 5)
186. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
187. Western blotting transfer - (reply: 2)
188. Western Quantification (No BCA???) - (reply: 2)
189. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
190. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
191. Trouble shooting in Far-western blot. - (reply: 1)
192. Transcription/Translation reaction not resolving - (reply: 7)
193. Ponceau red problem: membrane turned red - (reply: 3)
194. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
195. No bands/weird bands on western blot - (reply: 3)
196. Protein ladder not separating - (reply: 6)
197. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
198. Preparing supernatant from cell culture for western blot - (reply: 4)
199. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
200. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
201. Curved bands in Western Blot - (reply: 2)
202. Some bands not coming up, others uneven - (reply: 9)
203. Western blot and Triton X-100 in blocking buffer - (reply: 1)
204. Dot blot translation to WB - (reply: 2)
205. Reference protein give different expression - (reply: 3)
206. Excess betamercaptoethanol during sample preparation - (reply: 4)
207. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
208. Far western blot: probe concentration - (reply: 1)
209. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
210. Low Protein Content In Cell Lysate - (reply: 1)

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