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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. Western Blot with fixed cells - (reply: 5)
182. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
183. Primary antibody - (reply: 1)
184. Native-PAGE, help! - (reply: 3)
185. Trouble with purified recombinant protein bands - (reply: 3)
186. Stain in electrophoresis is going from + to - - (reply: 3)
187. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
188. Extraction of Transmembrane Proteins - (reply: 1)
189. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
190. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
191. Protein amount to load for publication figure? - (reply: 4)
192. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
193. What is different between Native protein and Denatured protein samples. - (reply: 4)
194. 3 extra bands show up on my western after an IP - (reply: 2)
195. IgG heavy chain from IP - (reply: 8)
196. about western blot analysis - (reply: 1)
197. Western Blotting loading control for large proteins - (reply: 2)
198. Uneven beta actin - (reply: 2)
199. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
200. NCOR1 western - (reply: 8)
201. Discrepancy between WB and Flow cytometry result - (reply: 5)
202. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
203. Western blotting transfer - (reply: 2)
204. Western Quantification (No BCA???) - (reply: 2)
205. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
206. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
207. Trouble shooting in Far-western blot. - (reply: 1)
208. Transcription/Translation reaction not resolving - (reply: 7)
209. Ponceau red problem: membrane turned red - (reply: 3)
210. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)

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