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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. Ponceau red problem: membrane turned red - (reply: 3)
182. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
183. No bands/weird bands on western blot - (reply: 3)
184. Protein ladder not separating - (reply: 6)
185. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
186. Preparing supernatant from cell culture for western blot - (reply: 4)
187. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
188. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
189. Curved bands in Western Blot - (reply: 2)
190. Some bands not coming up, others uneven - (reply: 9)
191. Western blot and Triton X-100 in blocking buffer - (reply: 1)
192. Dot blot translation to WB - (reply: 2)
193. Reference protein give different expression - (reply: 3)
194. Excess betamercaptoethanol during sample preparation - (reply: 4)
195. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
196. Far western blot: probe concentration - (reply: 1)
197. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
198. Low Protein Content In Cell Lysate - (reply: 1)
199. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)
200. B- actin band looks like dumbbells! - (reply: 2)
201. western blot explenation - (reply: 8)
202. WB of cellular proteins in human serum - (reply: 2)
203. Western blotting - (reply: 9)
204. How long can I store cell lysis buffer at 4C for? - (reply: 1)
205. Which antibody is good for HA-tag with low background bands? - (reply: 1)
206. Blocking solution mistake, please help! - (reply: 2)
207. Membrane Protein SDS PAGE and WB - (reply: 1)
208. Wet transfer of proteins between 100-200kDa - (reply: 2)
209. western blot and actin issue - (reply: 2)
210. Low-temperature SDS-PAGE: tips and suggestions - (reply: 4)

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