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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. Preparing supernatant from cell culture for western blot - (reply: 4)
182. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
183. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
184. Curved bands in Western Blot - (reply: 2)
185. Some bands not coming up, others uneven - (reply: 9)
186. Western blot and Triton X-100 in blocking buffer - (reply: 1)
187. Dot blot translation to WB - (reply: 2)
188. Reference protein give different expression - (reply: 3)
189. Excess betamercaptoethanol during sample preparation - (reply: 4)
190. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
191. Far western blot: probe concentration - (reply: 1)
192. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
193. Low Protein Content In Cell Lysate - (reply: 1)
194. HELP making 6X SDS Loading Buffer for SDS-PAGE - (reply: 3)
195. B- actin band looks like dumbbells! - (reply: 2)
196. western blot explenation - (reply: 8)
197. WB of cellular proteins in human serum - (reply: 2)
198. Western blotting - (reply: 9)
199. How long can I store cell lysis buffer at 4C for? - (reply: 1)
200. Which antibody is good for HA-tag with low background bands? - (reply: 1)
201. Blocking solution mistake, please help! - (reply: 2)
202. Membrane Protein SDS PAGE and WB - (reply: 1)
203. Wet transfer of proteins between 100-200kDa - (reply: 2)
204. western blot and actin issue - (reply: 2)
205. Low-temperature SDS-PAGE: tips and suggestions - (reply: 4)
206. conjugated antibodies in immunoprecipitation - (reply: 3)
207. Separating proteing with difference of 5Kd on western blot - (reply: 4)
208. small proteins - (reply: 2)
209. SDS PAGE gel storing - (reply: 3)
210. Interpretation & Troubleshooting on SDS/Native PAGE - (reply: 5)

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