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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. Detection of T7 RNA polymerase by WB - (reply: 6)
182. Shift in the position of band onto the western blot - (reply: 3)
183. Ghost and inverse bands and high background. Help! - (reply: 3)
184. No resolving of protein/bands below 50kDa :-s - (reply: 5)
185. Problems with wet transfer blot - (reply: 2)
186. 5% BSA blocking buffer - (reply: 3)
187. Storage of Membrane before addition of primary antibody - (reply: 3)
188. "Mickey Mouse" bands - (reply: 5)
189. SDS-PAGE : when the transfer stops - (reply: 1)
190. confusing Western results - (reply: 5)
191. aditional band on WB - (reply: 1)
192. Transferring >150 kDA proteins conditions - (reply: 3)
193. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
194. TAU gel electrophoresis of histones - (reply: 4)
195. Bands dark on sides and light in middle - (reply: 7)
196. Addition of sample loading buffer, before or after heating - (reply: 5)
197. question about protein expression normalization. - (reply: 6)
198. Protein bands not migrating - (reply: 3)
199. Help with high background (have tried most trouble shooting) - (reply: 8)
200. Tumor protein precipitation at denaturing step? - (reply: 1)
201. How to prepare blood serum for western blot? - (reply: 3)
202. TNF alpha and IL 1b Western Blot - (reply: 1)
203. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
204. SDS-PAGE trouble with protein concentration - (reply: 2)
205. Acrylamid - (reply: 5)
206. Detecting doublet bands in Western for eGFP control - (reply: 1)
207. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
208. Protein Size 50 kDa higher than expected - (reply: 3)
209. Acid urea gel electrophoresis (AUGE) - (reply: 7)
210. Phospho-protein vs total protein Western blot - (reply: 5)

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