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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. Insufficient Transfer of proteins through semi dry transfer - (reply: 6)
182. Smeared Bands after Ponceau Staining? - (reply: 3)
183. If preimmune sera and immune sera are both reacting to recombinat antigen in wes - (reply: 7)
184. patchy/holey bands after transfer, invasive bubbles? - (reply: 7)
185. Western not working - (reply: 4)
186. fused bands of beta-actin in western blot - (reply: 2)
187. Suggestions - Nice Western Blot Image from Office Scanner? - (reply: 7)
188. Undergrad "activated" a NS membrane with 100% MeOH... Any hope? - (reply: 5)
189. Problem with GFP detection in western - (reply: 6)
190. Sudden Trouble with PVDF Transfer - (reply: 6)
191. Equal Loading Confusion - (reply: 6)
192. Wrong protein size on western blot - (reply: 10)
193. How to know if the signal of western blot is saturated ? - (reply: 4)
194. Scotch-Brite versus other foam pad for wet transfer? - (reply: 3)
195. 5x SDS lysis buffer - (reply: 5)
196. Protein measurement of Laemmli samples - (reply: 2)
197. SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
198. Uneven banding - Western blot troubleshooting - (reply: 4)
199. Western Blot with fixed cells - (reply: 5)
200. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
201. Primary antibody - (reply: 1)
202. Native-PAGE, help! - (reply: 3)
203. Trouble with purified recombinant protein bands - (reply: 3)
204. Stain in electrophoresis is going from + to - - (reply: 3)
205. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
206. Extraction of Transmembrane Proteins - (reply: 1)
207. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
208. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
209. Protein amount to load for publication figure? - (reply: 4)
210. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)

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