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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. Lysis buffer - (reply: 2)
182. SDS Page and WB: Wrong size bands - (reply: 2)
183. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
184. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
185. How to avoid gel breakage after electrophoresis - (reply: 5)
186. What is an Input in SDS-PAGE? - (reply: 1)
187. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
188. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
189. Good loading control for native gels? - (reply: 1)
190. Please help with odd bands and smears on Western blot - (reply: 4)
191. How to load equal amount of protein without using spectramax - (reply: 4)
192. Western blot membrane imaging - (reply: 7)
193. Detection of T7 RNA polymerase by WB - (reply: 6)
194. Shift in the position of band onto the western blot - (reply: 3)
195. Ghost and inverse bands and high background. Help! - (reply: 3)
196. No resolving of protein/bands below 50kDa :-s - (reply: 5)
197. Problems with wet transfer blot - (reply: 2)
198. 5% BSA blocking buffer - (reply: 3)
199. Storage of Membrane before addition of primary antibody - (reply: 3)
200. "Mickey Mouse" bands - (reply: 5)
201. SDS-PAGE : when the transfer stops - (reply: 1)
202. confusing Western results - (reply: 5)
203. aditional band on WB - (reply: 1)
204. Transferring >150 kDA proteins conditions - (reply: 3)
205. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
206. TAU gel electrophoresis of histones - (reply: 4)
207. Bands dark on sides and light in middle - (reply: 7)
208. Addition of sample loading buffer, before or after heating - (reply: 5)
209. question about protein expression normalization. - (reply: 6)
210. Protein bands not migrating - (reply: 3)

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