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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. "Wavy" bands in SDS-PAGE gel - (reply: 5)
182. LMW proteins not transfer of gel to membrane - (reply: 1)
183. smear in SDS PAGE - (reply: 4)
184. Insufficient Transfer of proteins through semi dry transfer - (reply: 6)
185. Smeared Bands after Ponceau Staining? - (reply: 3)
186. If preimmune sera and immune sera are both reacting to recombinat antigen in wes - (reply: 7)
187. patchy/holey bands after transfer, invasive bubbles? - (reply: 7)
188. Western not working - (reply: 4)
189. fused bands of beta-actin in western blot - (reply: 2)
190. Suggestions - Nice Western Blot Image from Office Scanner? - (reply: 7)
191. Undergrad "activated" a NS membrane with 100% MeOH... Any hope? - (reply: 5)
192. Problem with GFP detection in western - (reply: 6)
193. Sudden Trouble with PVDF Transfer - (reply: 6)
194. Equal Loading Confusion - (reply: 6)
195. Wrong protein size on western blot - (reply: 10)
196. How to know if the signal of western blot is saturated ? - (reply: 4)
197. Scotch-Brite versus other foam pad for wet transfer? - (reply: 3)
198. 5x SDS lysis buffer - (reply: 5)
199. Protein measurement of Laemmli samples - (reply: 2)
200. SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
201. Uneven banding - Western blot troubleshooting - (reply: 4)
202. Western Blot with fixed cells - (reply: 5)
203. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
204. Primary antibody - (reply: 1)
205. Native-PAGE, help! - (reply: 3)
206. Trouble with purified recombinant protein bands - (reply: 3)
207. Stain in electrophoresis is going from + to - - (reply: 3)
208. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
209. Extraction of Transmembrane Proteins - (reply: 1)
210. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)

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