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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. What is different between Native protein and Denatured protein samples. - (reply: 4)
182. 3 extra bands show up on my western after an IP - (reply: 2)
183. IgG heavy chain from IP - (reply: 8)
184. about western blot analysis - (reply: 1)
185. Western Blotting loading control for large proteins - (reply: 2)
186. Uneven beta actin - (reply: 2)
187. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
188. NCOR1 western - (reply: 8)
189. Discrepancy between WB and Flow cytometry result - (reply: 5)
190. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
191. Western blotting transfer - (reply: 2)
192. Western Quantification (No BCA???) - (reply: 2)
193. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
194. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
195. Trouble shooting in Far-western blot. - (reply: 1)
196. Transcription/Translation reaction not resolving - (reply: 7)
197. Ponceau red problem: membrane turned red - (reply: 3)
198. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
199. No bands/weird bands on western blot - (reply: 3)
200. Protein ladder not separating - (reply: 6)
201. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
202. Preparing supernatant from cell culture for western blot - (reply: 4)
203. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
204. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
205. Curved bands in Western Blot - (reply: 2)
206. Some bands not coming up, others uneven - (reply: 9)
207. Western blot and Triton X-100 in blocking buffer - (reply: 1)
208. Dot blot translation to WB - (reply: 2)
209. Reference protein give different expression - (reply: 3)
210. Excess betamercaptoethanol during sample preparation - (reply: 4)

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