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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. Problem with SDS-PAGE gel - (reply: 13)
182. How to dissolve TCA/acetone precipitated proteins? - (reply: 1)
183. SDS-Page trouble-shooting - (reply: 3)
184. PVDF membrane long-term storage after transfer - (reply: 3)
185. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
186. Distorted bands from human colon samples - (reply: 2)
187. "Wavy" bands in SDS-PAGE gel - (reply: 5)
188. LMW proteins not transfer of gel to membrane - (reply: 1)
189. smear in SDS PAGE - (reply: 4)
190. Insufficient Transfer of proteins through semi dry transfer - (reply: 6)
191. Smeared Bands after Ponceau Staining? - (reply: 3)
192. If preimmune sera and immune sera are both reacting to recombinat antigen in wes - (reply: 7)
193. patchy/holey bands after transfer, invasive bubbles? - (reply: 7)
194. Western not working - (reply: 4)
195. fused bands of beta-actin in western blot - (reply: 2)
196. Suggestions - Nice Western Blot Image from Office Scanner? - (reply: 7)
197. Undergrad "activated" a NS membrane with 100% MeOH... Any hope? - (reply: 5)
198. Problem with GFP detection in western - (reply: 6)
199. Sudden Trouble with PVDF Transfer - (reply: 6)
200. Equal Loading Confusion - (reply: 6)
201. Wrong protein size on western blot - (reply: 10)
202. How to know if the signal of western blot is saturated ? - (reply: 4)
203. Scotch-Brite versus other foam pad for wet transfer? - (reply: 3)
204. 5x SDS lysis buffer - (reply: 5)
205. Protein measurement of Laemmli samples - (reply: 2)
206. SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
207. Uneven banding - Western blot troubleshooting - (reply: 4)
208. Western Blot with fixed cells - (reply: 5)
209. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
210. Primary antibody - (reply: 1)

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