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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
181. Semi-dry system for NuPage with MES buffer - (reply: 4)
182. Recommend anti-rat HRP secondary antibody - (reply: 2)
183. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
184. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)
185. Why Squeezing of lanes occur at bottom in PAGE - (reply: 6)
186. Western blot to CD44? - (reply: 3)
187. Amount of EDTA - (reply: 4)
188. SDS Apparatus for northern - (reply: 1)
189. Prey protein band bleeds, IgG band normal? - (reply: 3)
190. Coomassie gel after semi-dry transfer - normal like this? - (reply: 7)
191. His-Probe - (reply: 2)
192. Problems with western membrane stripping - (reply: 2)
193. Can protein isomers be detected in SDS-PAGE - (reply: 3)
194. western blot of cardiomyocytes - (reply: 2)
195. Gradient Gel Electrophoresis - (reply: 3)
196. SDS-PAGE resolving gel not completely set - (reply: 4)
197. Problem with SDS-PAGE gel - (reply: 13)
198. How to dissolve TCA/acetone precipitated proteins? - (reply: 1)
199. SDS-Page trouble-shooting - (reply: 3)
200. PVDF membrane long-term storage after transfer - (reply: 3)
201. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
202. Distorted bands from human colon samples - (reply: 2)
203. "Wavy" bands in SDS-PAGE gel - (reply: 5)
204. LMW proteins not transfer of gel to membrane - (reply: 1)
205. smear in SDS PAGE - (reply: 4)
206. Insufficient Transfer of proteins through semi dry transfer - (reply: 6)
207. Smeared Bands after Ponceau Staining? - (reply: 3)
208. If preimmune sera and immune sera are both reacting to recombinat antigen in wes - (reply: 7)
209. patchy/holey bands after transfer, invasive bubbles? - (reply: 7)
210. Western not working - (reply: 4)

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