751. Help!! Uneven actin expression . - Thick and thin actin expression. (reply: 1)
753. Lost protein from blot? - (reply: 1)
755. No SDS in gels - (reply: 12)
757. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
759. western bands look funny - (reply: 5)
761. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
763. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
765. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
767. problems with His tagged protein western blots - (reply: 3)
769. Methanol vs Isopropanol in fixing solution - (reply: 1)
771. probably DNA is disturbing the quantification of my sample - (reply: 3)
773. Optimising antibody concentration - hints and tips (reply: 1)
775. Protein Markers - (reply: 1)
777. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
779. Immunoprecipitation - (reply: 2)

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