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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. SEmi-dry transfer blotting - (reply: 1)
752. Whole cell lysis of fibroblasts - (reply: 1)
753. SDS-PAGE: Strange Running - (reply: 8)
754. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
755. Different TBST pH's for WB incubation, why? - (reply: 1)
756. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
757. stability of the stripping buffer - (reply: 2)
758. small proteins have run ahead of tracking dye - (reply: 1)
759. cytoplasmic and nuclear extraction - (reply: 2)
760. Equal Protein Quantity - Loading onto Well (reply: 6)
761. Help, odd pattern - pic included - (reply: 3)
762. Fermentas prestained protein marker issue! - (reply: 7)
763. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
764. Invitrogen NuPAGE Sample buffer - (reply: 1)
765. western exposure one part of band darker - (reply: 6)
766. non-specific bands - (reply: 5)
767. Crazy looking BN-PAGE gel - No, really. (reply: 5)
768. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
769. bands of protein seems like it did not move - (reply: 2)
770. downshifted band - (reply: 4)
771. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
772. Western Troubleshooting Help, please! - (reply: 3)
773. Re-use membrane in Western Blot - (reply: 3)
774. NP40 or Triton as membrane protein detergent - (reply: 2)
775. Dot blot- great, western blot - no signal - (reply: 3)
776. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
777. Problem in transfer high MW protein - (reply: 5)
778. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
779. dimerization or aggregation of my protein! - (reply: 3)
780. basic question in SDS and western blot - (reply: 1)

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