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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. Invitrogen NuPAGE Sample buffer - (reply: 1)
752. western exposure one part of band darker - (reply: 6)
753. non-specific bands - (reply: 5)
754. Crazy looking BN-PAGE gel - No, really. (reply: 5)
755. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
756. bands of protein seems like it did not move - (reply: 2)
757. downshifted band - (reply: 4)
758. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
759. Western Troubleshooting Help, please! - (reply: 3)
760. Re-use membrane in Western Blot - (reply: 3)
761. NP40 or Triton as membrane protein detergent - (reply: 2)
762. Dot blot- great, western blot - no signal - (reply: 3)
763. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
764. Problem in transfer high MW protein - (reply: 5)
765. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
766. dimerization or aggregation of my protein! - (reply: 3)
767. basic question in SDS and western blot - (reply: 1)
768. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
769. Transfer problems - (reply: 1)
770. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
771. Stripping temperature - have no 55C water bath (reply: 1)
772. Nonspecificity of anti-flag - (reply: 2)
773. Blocking buffer - (reply: 7)
774. Detection of phospho-proteins - (reply: 1)
775. light chain - (reply: 5)
776. ugly merged bands in western blot - (reply: 3)
777. Western Blot Data Analysis - (reply: 2)
778. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
779. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
780. Protein Expression in stationary phase - (reply: 11)

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