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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. Problem with protein samples - No bands detected at all on gel (reply: 2)
752. using cell lysate to run native gel? - (reply: 1)
753. Bradford Query - (reply: 5)
754. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
755. About GFP- tagged proteins in western blot - (reply: 1)
756. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
757. PVP-40 for blocking? - Instead of milk (reply: 2)
758. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
759. How to pase my Co-IP over the weekend? - (reply: 1)
760. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
761. Protein Sample Preparation - Western Bloting (reply: 1)
762. No signal at all - (reply: 16)
763. Centrifuge after lysing - (reply: 5)
764. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)
765. "Diffuesed" bands of higher molecular weight - (reply: 6)
766. Dimeric form of a protein? - I want my monomer! (reply: 2)
767. PBS in my Tris Glycine Running Buffer....what will happen - (reply: 3)
768. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
769. how to get rabbit antibody for Western blotting - (reply: 2)
770. voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
771. Not sure of what % to use for gels - (reply: 6)
772. Protein Size - (reply: 3)
773. no separation of MW marker on gel - (reply: 1)
774. Transfer basic protein to PVDF - (reply: 1)
775. Expired pre-cast gels - (reply: 4)
776. LDS sample buffer in 95C - (reply: 2)
777. disappearing high molecular protein and marker,thanks - (reply: 1)
778. Use of cold milk??? - Western blot (reply: 2)
779. Antibody compatibility between different species - (reply: 4)
780. "weber & osborne" and neville's SDS-PAGE method - protocol please (reply: 6)

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