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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
752. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
753. Sample loading buffer turning yellow. - (reply: 3)
754. western bands look funny - (reply: 5)
755. IgG SDS-PAGE - (reply: 1)
756. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
757. wet or semi-dry transfer for low MW proteins - (reply: 4)
758. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
759. quantifying "ghost" bands - (reply: 1)
760. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
761. Western blot running and loading samples - (reply: 6)
762. problems with His tagged protein western blots - (reply: 3)
763. Springerlink--Zymography - (reply: 3)
764. Methanol vs Isopropanol in fixing solution - (reply: 1)
765. Protein aggregates SDS-PAGE - (reply: 5)
766. probably DNA is disturbing the quantification of my sample - (reply: 3)
767. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
768. Optimising antibody concentration - hints and tips (reply: 1)
769. Lanes all run together - (reply: 5)
770. Protein Markers - (reply: 1)
771. Gel Running Crooked?? Please help.. - (reply: 7)
772. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
773. Strength of actin signal - How dark should the band be? (reply: 5)
774. Immunoprecipitation - (reply: 2)
775. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
776. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
777. SEmi-dry transfer blotting - (reply: 1)
778. Whole cell lysis of fibroblasts - (reply: 1)
779. SDS-PAGE: Strange Running - (reply: 8)
780. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)

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