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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. Protein aggregates SDS-PAGE - (reply: 5)
752. probably DNA is disturbing the quantification of my sample - (reply: 3)
753. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
754. Optimising antibody concentration - hints and tips (reply: 1)
755. Lanes all run together - (reply: 5)
756. Protein Markers - (reply: 1)
757. Gel Running Crooked?? Please help.. - (reply: 7)
758. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
759. Strength of actin signal - How dark should the band be? (reply: 5)
760. Immunoprecipitation - (reply: 2)
761. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
762. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
763. SEmi-dry transfer blotting - (reply: 1)
764. Whole cell lysis of fibroblasts - (reply: 1)
765. SDS-PAGE: Strange Running - (reply: 8)
766. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
767. Different TBST pH's for WB incubation, why? - (reply: 1)
768. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
769. stability of the stripping buffer - (reply: 2)
770. small proteins have run ahead of tracking dye - (reply: 1)
771. cytoplasmic and nuclear extraction - (reply: 2)
772. Equal Protein Quantity - Loading onto Well (reply: 6)
773. Help, odd pattern - pic included - (reply: 3)
774. Fermentas prestained protein marker issue! - (reply: 7)
775. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
776. Invitrogen NuPAGE Sample buffer - (reply: 1)
777. western exposure one part of band darker - (reply: 6)
778. non-specific bands - (reply: 5)
779. Crazy looking BN-PAGE gel - No, really. (reply: 5)
780. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)

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