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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
752. quantifying "ghost" bands - (reply: 1)
753. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
754. Western blot running and loading samples - (reply: 6)
755. problems with His tagged protein western blots - (reply: 3)
756. Springerlink--Zymography - (reply: 3)
757. Methanol vs Isopropanol in fixing solution - (reply: 1)
758. Protein aggregates SDS-PAGE - (reply: 5)
759. probably DNA is disturbing the quantification of my sample - (reply: 3)
760. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
761. Optimising antibody concentration - hints and tips (reply: 1)
762. Lanes all run together - (reply: 5)
763. Protein Markers - (reply: 1)
764. Gel Running Crooked?? Please help.. - (reply: 7)
765. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
766. Strength of actin signal - How dark should the band be? (reply: 5)
767. Immunoprecipitation - (reply: 2)
768. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
769. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
770. SEmi-dry transfer blotting - (reply: 1)
771. Whole cell lysis of fibroblasts - (reply: 1)
772. SDS-PAGE: Strange Running - (reply: 8)
773. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
774. Different TBST pH's for WB incubation, why? - (reply: 1)
775. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
776. stability of the stripping buffer - (reply: 2)
777. small proteins have run ahead of tracking dye - (reply: 1)
778. cytoplasmic and nuclear extraction - (reply: 2)
779. Equal Protein Quantity - Loading onto Well (reply: 6)
780. Help, odd pattern - pic included - (reply: 3)

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