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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. NUCLEAR PROTEIN PREPARATION - (reply: 1)
752. Lost protein from blot? - (reply: 1)
753. Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
754. No SDS in gels - (reply: 12)
755. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
756. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
757. Sample loading buffer turning yellow. - (reply: 3)
758. western bands look funny - (reply: 5)
759. IgG SDS-PAGE - (reply: 1)
760. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
761. wet or semi-dry transfer for low MW proteins - (reply: 4)
762. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
763. quantifying "ghost" bands - (reply: 1)
764. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
765. Western blot running and loading samples - (reply: 6)
766. problems with His tagged protein western blots - (reply: 3)
767. Springerlink--Zymography - (reply: 3)
768. Methanol vs Isopropanol in fixing solution - (reply: 1)
769. Protein aggregates SDS-PAGE - (reply: 5)
770. probably DNA is disturbing the quantification of my sample - (reply: 3)
771. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
772. Optimising antibody concentration - hints and tips (reply: 1)
773. Lanes all run together - (reply: 5)
774. Protein Markers - (reply: 1)
775. Gel Running Crooked?? Please help.. - (reply: 7)
776. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
777. Strength of actin signal - How dark should the band be? (reply: 5)
778. Immunoprecipitation - (reply: 2)
779. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
780. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)

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