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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. Strength of actin signal - How dark should the band be? (reply: 5)
752. Immunoprecipitation - (reply: 2)
753. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
754. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
755. SEmi-dry transfer blotting - (reply: 1)
756. Whole cell lysis of fibroblasts - (reply: 1)
757. SDS-PAGE: Strange Running - (reply: 8)
758. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
759. Different TBST pH's for WB incubation, why? - (reply: 1)
760. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
761. stability of the stripping buffer - (reply: 2)
762. small proteins have run ahead of tracking dye - (reply: 1)
763. cytoplasmic and nuclear extraction - (reply: 2)
764. Equal Protein Quantity - Loading onto Well (reply: 6)
765. Help, odd pattern - pic included - (reply: 3)
766. Fermentas prestained protein marker issue! - (reply: 7)
767. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
768. Invitrogen NuPAGE Sample buffer - (reply: 1)
769. western exposure one part of band darker - (reply: 6)
770. non-specific bands - (reply: 5)
771. Crazy looking BN-PAGE gel - No, really. (reply: 5)
772. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
773. bands of protein seems like it did not move - (reply: 2)
774. downshifted band - (reply: 4)
775. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
776. Western Troubleshooting Help, please! - (reply: 3)
777. Re-use membrane in Western Blot - (reply: 3)
778. NP40 or Triton as membrane protein detergent - (reply: 2)
779. Dot blot- great, western blot - no signal - (reply: 3)
780. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)

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