Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
752. Optimising antibody concentration - hints and tips (reply: 1)
753. Lanes all run together - (reply: 5)
754. Protein Markers - (reply: 1)
755. Gel Running Crooked?? Please help.. - (reply: 7)
756. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
757. Strength of actin signal - How dark should the band be? (reply: 5)
758. Immunoprecipitation - (reply: 2)
759. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
760. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
761. SEmi-dry transfer blotting - (reply: 1)
762. Whole cell lysis of fibroblasts - (reply: 1)
763. SDS-PAGE: Strange Running - (reply: 8)
764. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
765. Different TBST pH's for WB incubation, why? - (reply: 1)
766. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
767. stability of the stripping buffer - (reply: 2)
768. small proteins have run ahead of tracking dye - (reply: 1)
769. cytoplasmic and nuclear extraction - (reply: 2)
770. Equal Protein Quantity - Loading onto Well (reply: 6)
771. Help, odd pattern - pic included - (reply: 3)
772. Fermentas prestained protein marker issue! - (reply: 7)
773. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
774. Invitrogen NuPAGE Sample buffer - (reply: 1)
775. western exposure one part of band darker - (reply: 6)
776. non-specific bands - (reply: 5)
777. Crazy looking BN-PAGE gel - No, really. (reply: 5)
778. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
779. bands of protein seems like it did not move - (reply: 2)
780. downshifted band - (reply: 4)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37