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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
752. wet or semi-dry transfer for low MW proteins - (reply: 4)
753. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
754. quantifying "ghost" bands - (reply: 1)
755. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
756. Western blot running and loading samples - (reply: 6)
757. problems with His tagged protein western blots - (reply: 3)
758. Springerlink--Zymography - (reply: 3)
759. Methanol vs Isopropanol in fixing solution - (reply: 1)
760. Protein aggregates SDS-PAGE - (reply: 5)
761. probably DNA is disturbing the quantification of my sample - (reply: 3)
762. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
763. Optimising antibody concentration - hints and tips (reply: 1)
764. Lanes all run together - (reply: 5)
765. Protein Markers - (reply: 1)
766. Gel Running Crooked?? Please help.. - (reply: 7)
767. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
768. Strength of actin signal - How dark should the band be? (reply: 5)
769. Immunoprecipitation - (reply: 2)
770. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
771. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
772. SEmi-dry transfer blotting - (reply: 1)
773. Whole cell lysis of fibroblasts - (reply: 1)
774. SDS-PAGE: Strange Running - (reply: 8)
775. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
776. Different TBST pH's for WB incubation, why? - (reply: 1)
777. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
778. stability of the stripping buffer - (reply: 2)
779. small proteins have run ahead of tracking dye - (reply: 1)
780. cytoplasmic and nuclear extraction - (reply: 2)

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