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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
751. western bands look funny - (reply: 5)
752. IgG SDS-PAGE - (reply: 1)
753. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
754. wet or semi-dry transfer for low MW proteins - (reply: 4)
755. is it ok to use EtOH or MeOH instead of Butanol? - for sds-page, ran out of butaol (reply: 3)
756. quantifying "ghost" bands - (reply: 1)
757. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
758. Western blot running and loading samples - (reply: 6)
759. problems with His tagged protein western blots - (reply: 3)
760. Springerlink--Zymography - (reply: 3)
761. Methanol vs Isopropanol in fixing solution - (reply: 1)
762. Protein aggregates SDS-PAGE - (reply: 5)
763. probably DNA is disturbing the quantification of my sample - (reply: 3)
764. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
765. Optimising antibody concentration - hints and tips (reply: 1)
766. Lanes all run together - (reply: 5)
767. Protein Markers - (reply: 1)
768. Gel Running Crooked?? Please help.. - (reply: 7)
769. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
770. Strength of actin signal - How dark should the band be? (reply: 5)
771. Immunoprecipitation - (reply: 2)
772. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
773. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
774. SEmi-dry transfer blotting - (reply: 1)
775. Whole cell lysis of fibroblasts - (reply: 1)
776. SDS-PAGE: Strange Running - (reply: 8)
777. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
778. Different TBST pH's for WB incubation, why? - (reply: 1)
779. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
780. stability of the stripping buffer - (reply: 2)

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