|
931. direct lysis in Laemmli buffer - (reply: 4)
932. anti-FLAG (M2) cross reaction - (reply: 6)
933. NFkB western blotting - (reply: 3)
934. Mannitol in Lysis Buffer - (reply: 1)
935. how long will a 1M DTT solution will last in -20C - (reply: 5)
936. pellet became insoluble after boiling. - (reply: 4)
937. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
938. Ladder but no protein - (reply: 4)
939. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
940. my membranes are blue! - silly mistake, what to do? (reply: 1)
941. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
942. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
943. Primary Antibody for SPDEF - (reply: 1)
944. Detecting Patterns of phosphorylated protein - (reply: 1)
945. Sample Diffusion Before Applying Current - (reply: 4)
946. IP and western blot - (reply: 5)
947. Semi-dry transfer conditions - Volts or Amps (reply: 1)
948. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
949. Destain after PoneauS - (reply: 6)
950. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
951. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
952. Variability in GAPDH - (reply: 5)
953. nitrocellulose or PVDF - (reply: 7)
954. crappy bands after developing - (reply: 3)
955. cloudy gel - (reply: 2)
956. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
957. Beginner Question - (reply: 6)
958. What's the problem with this SDS-PAGE? - (reply: 8)
959. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
960. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
932. anti-FLAG (M2) cross reaction - (reply: 6)
933. NFkB western blotting - (reply: 3)
934. Mannitol in Lysis Buffer - (reply: 1)
935. how long will a 1M DTT solution will last in -20C - (reply: 5)
936. pellet became insoluble after boiling. - (reply: 4)
937. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
938. Ladder but no protein - (reply: 4)
939. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
940. my membranes are blue! - silly mistake, what to do? (reply: 1)
941. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
942. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
943. Primary Antibody for SPDEF - (reply: 1)
944. Detecting Patterns of phosphorylated protein - (reply: 1)
945. Sample Diffusion Before Applying Current - (reply: 4)
946. IP and western blot - (reply: 5)
947. Semi-dry transfer conditions - Volts or Amps (reply: 1)
948. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
949. Destain after PoneauS - (reply: 6)
950. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
951. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
952. Variability in GAPDH - (reply: 5)
953. nitrocellulose or PVDF - (reply: 7)
954. crappy bands after developing - (reply: 3)
955. cloudy gel - (reply: 2)
956. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
957. Beginner Question - (reply: 6)
958. What's the problem with this SDS-PAGE? - (reply: 8)
959. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
960. Antibody Validation - Please help me! THis is very Urgent (reply: 2)