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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
931. pellet became insoluble after boiling. - (reply: 4)
932. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
933. Ladder but no protein - (reply: 4)
934. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
935. my membranes are blue! - silly mistake, what to do? (reply: 1)
936. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
937. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
938. Primary Antibody for SPDEF - (reply: 1)
939. Detecting Patterns of phosphorylated protein - (reply: 1)
940. Sample Diffusion Before Applying Current - (reply: 4)
941. IP and western blot - (reply: 5)
942. Semi-dry transfer conditions - Volts or Amps (reply: 1)
943. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
944. Destain after PoneauS - (reply: 6)
945. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
946. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
947. Variability in GAPDH - (reply: 5)
948. nitrocellulose or PVDF - (reply: 7)
949. crappy bands after developing - (reply: 3)
950. cloudy gel - (reply: 2)
951. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
952. Beginner Question - (reply: 6)
953. What's the problem with this SDS-PAGE? - (reply: 8)
954. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
955. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
956. Stripping question - (reply: 3)
957. Smears after transfer - (reply: 2)
958. Accidentally Froze Transfer Buffer - (reply: 1)
959. ImageJ analysis on IFs - (reply: 11)
960. Equal loading for Supernatant western blots??? - (reply: 1)

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