Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
931. Large protein transfer problem - (reply: 8)
932. overloading GAPDH but nice band of interest - (reply: 2)
933. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
934. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
935. Quantiying bands. - Determination of protein contens (reply: 2)
936. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
937. Strange lane pattern, yes/no pattern - (reply: 3)
938. Native PAGE w. Western protein marker - (reply: 4)
939. protein stuck - (reply: 3)
940. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
941. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
942. changing colour of front dye in sds-page - (reply: 10)
943. small protein blotting - (reply: 8)
944. how stable is alpha mouse HRP? - left on the bench (reply: 2)
945. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
946. Too many bands in my SDS-PAGE - (reply: 5)
947. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
948. Uneven bands in SDS-PAGE - (reply: 5)
949. Can primary antibody be reused ? - (reply: 6)
950. Preparation of tree tissue for western blot - (reply: 1)
951. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
952. direct lysis in Laemmli buffer - (reply: 4)
953. anti-FLAG (M2) cross reaction - (reply: 6)
954. NFkB western blotting - (reply: 3)
955. Mannitol in Lysis Buffer - (reply: 1)
956. how long will a 1M DTT solution will last in -20C - (reply: 5)
957. pellet became insoluble after boiling. - (reply: 4)
958. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
959. Ladder but no protein - (reply: 4)
960. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37