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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
931. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
932. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
933. changing colour of front dye in sds-page - (reply: 10)
934. small protein blotting - (reply: 8)
935. how stable is alpha mouse HRP? - left on the bench (reply: 2)
936. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
937. Too many bands in my SDS-PAGE - (reply: 5)
938. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
939. Uneven bands in SDS-PAGE - (reply: 5)
940. Can primary antibody be reused ? - (reply: 6)
941. Preparation of tree tissue for western blot - (reply: 1)
942. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
943. direct lysis in Laemmli buffer - (reply: 4)
944. anti-FLAG (M2) cross reaction - (reply: 6)
945. NFkB western blotting - (reply: 3)
946. Mannitol in Lysis Buffer - (reply: 1)
947. how long will a 1M DTT solution will last in -20C - (reply: 5)
948. pellet became insoluble after boiling. - (reply: 4)
949. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
950. Ladder but no protein - (reply: 4)
951. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
952. my membranes are blue! - silly mistake, what to do? (reply: 1)
953. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
954. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
955. Primary Antibody for SPDEF - (reply: 1)
956. Detecting Patterns of phosphorylated protein - (reply: 1)
957. Sample Diffusion Before Applying Current - (reply: 4)
958. IP and western blot - (reply: 5)
959. Semi-dry transfer conditions - Volts or Amps (reply: 1)
960. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)

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