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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
931. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
932. direct lysis in Laemmli buffer - (reply: 4)
933. anti-FLAG (M2) cross reaction - (reply: 6)
934. NFkB western blotting - (reply: 3)
935. Mannitol in Lysis Buffer - (reply: 1)
936. how long will a 1M DTT solution will last in -20C - (reply: 5)
937. pellet became insoluble after boiling. - (reply: 4)
938. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
939. Ladder but no protein - (reply: 4)
940. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
941. my membranes are blue! - silly mistake, what to do? (reply: 1)
942. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
943. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
944. Primary Antibody for SPDEF - (reply: 1)
945. Detecting Patterns of phosphorylated protein - (reply: 1)
946. Sample Diffusion Before Applying Current - (reply: 4)
947. IP and western blot - (reply: 5)
948. Semi-dry transfer conditions - Volts or Amps (reply: 1)
949. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
950. Destain after PoneauS - (reply: 6)
951. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
952. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
953. Variability in GAPDH - (reply: 5)
954. nitrocellulose or PVDF - (reply: 7)
955. crappy bands after developing - (reply: 3)
956. cloudy gel - (reply: 2)
957. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
958. Beginner Question - (reply: 6)
959. What's the problem with this SDS-PAGE? - (reply: 8)
960. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)

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