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931. Preparation of tree tissue for western blot - (reply: 1)
932. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
933. direct lysis in Laemmli buffer - (reply: 4)
934. anti-FLAG (M2) cross reaction - (reply: 6)
935. NFkB western blotting - (reply: 3)
936. Mannitol in Lysis Buffer - (reply: 1)
937. how long will a 1M DTT solution will last in -20C - (reply: 5)
938. pellet became insoluble after boiling. - (reply: 4)
939. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
940. Ladder but no protein - (reply: 4)
941. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
942. my membranes are blue! - silly mistake, what to do? (reply: 1)
943. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
944. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
945. Primary Antibody for SPDEF - (reply: 1)
946. Detecting Patterns of phosphorylated protein - (reply: 1)
947. Sample Diffusion Before Applying Current - (reply: 4)
948. IP and western blot - (reply: 5)
949. Semi-dry transfer conditions - Volts or Amps (reply: 1)
950. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
951. Destain after PoneauS - (reply: 6)
952. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
953. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
954. Variability in GAPDH - (reply: 5)
955. nitrocellulose or PVDF - (reply: 7)
956. crappy bands after developing - (reply: 3)
957. cloudy gel - (reply: 2)
958. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
959. Beginner Question - (reply: 6)
960. What's the problem with this SDS-PAGE? - (reply: 8)
932. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
933. direct lysis in Laemmli buffer - (reply: 4)
934. anti-FLAG (M2) cross reaction - (reply: 6)
935. NFkB western blotting - (reply: 3)
936. Mannitol in Lysis Buffer - (reply: 1)
937. how long will a 1M DTT solution will last in -20C - (reply: 5)
938. pellet became insoluble after boiling. - (reply: 4)
939. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
940. Ladder but no protein - (reply: 4)
941. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
942. my membranes are blue! - silly mistake, what to do? (reply: 1)
943. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
944. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
945. Primary Antibody for SPDEF - (reply: 1)
946. Detecting Patterns of phosphorylated protein - (reply: 1)
947. Sample Diffusion Before Applying Current - (reply: 4)
948. IP and western blot - (reply: 5)
949. Semi-dry transfer conditions - Volts or Amps (reply: 1)
950. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
951. Destain after PoneauS - (reply: 6)
952. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
953. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
954. Variability in GAPDH - (reply: 5)
955. nitrocellulose or PVDF - (reply: 7)
956. crappy bands after developing - (reply: 3)
957. cloudy gel - (reply: 2)
958. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
959. Beginner Question - (reply: 6)
960. What's the problem with this SDS-PAGE? - (reply: 8)