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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
931. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
932. nuclear loading control - (reply: 2)
933. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
934. Large protein transfer problem - (reply: 8)
935. overloading GAPDH but nice band of interest - (reply: 2)
936. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
937. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
938. Quantiying bands. - Determination of protein contens (reply: 2)
939. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
940. Strange lane pattern, yes/no pattern - (reply: 3)
941. Native PAGE w. Western protein marker - (reply: 4)
942. protein stuck - (reply: 3)
943. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
944. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
945. changing colour of front dye in sds-page - (reply: 10)
946. small protein blotting - (reply: 8)
947. how stable is alpha mouse HRP? - left on the bench (reply: 2)
948. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
949. Too many bands in my SDS-PAGE - (reply: 5)
950. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
951. Uneven bands in SDS-PAGE - (reply: 5)
952. Can primary antibody be reused ? - (reply: 6)
953. Preparation of tree tissue for western blot - (reply: 1)
954. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
955. direct lysis in Laemmli buffer - (reply: 4)
956. anti-FLAG (M2) cross reaction - (reply: 6)
957. NFkB western blotting - (reply: 3)
958. Mannitol in Lysis Buffer - (reply: 1)
959. how long will a 1M DTT solution will last in -20C - (reply: 5)
960. pellet became insoluble after boiling. - (reply: 4)

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