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931. nuclear loading control - (reply: 2)
932. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
933. Large protein transfer problem - (reply: 8)
934. overloading GAPDH but nice band of interest - (reply: 2)
935. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
936. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
937. Quantiying bands. - Determination of protein contens (reply: 2)
938. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
939. Strange lane pattern, yes/no pattern - (reply: 3)
940. Native PAGE w. Western protein marker - (reply: 4)
941. protein stuck - (reply: 3)
942. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
943. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
944. changing colour of front dye in sds-page - (reply: 10)
945. small protein blotting - (reply: 8)
946. how stable is alpha mouse HRP? - left on the bench (reply: 2)
947. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
948. Too many bands in my SDS-PAGE - (reply: 5)
949. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
950. Uneven bands in SDS-PAGE - (reply: 5)
951. Can primary antibody be reused ? - (reply: 6)
952. Preparation of tree tissue for western blot - (reply: 1)
953. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
954. direct lysis in Laemmli buffer - (reply: 4)
955. anti-FLAG (M2) cross reaction - (reply: 6)
956. NFkB western blotting - (reply: 3)
957. Mannitol in Lysis Buffer - (reply: 1)
958. how long will a 1M DTT solution will last in -20C - (reply: 5)
959. pellet became insoluble after boiling. - (reply: 4)
960. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
932. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
933. Large protein transfer problem - (reply: 8)
934. overloading GAPDH but nice band of interest - (reply: 2)
935. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
936. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
937. Quantiying bands. - Determination of protein contens (reply: 2)
938. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
939. Strange lane pattern, yes/no pattern - (reply: 3)
940. Native PAGE w. Western protein marker - (reply: 4)
941. protein stuck - (reply: 3)
942. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
943. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
944. changing colour of front dye in sds-page - (reply: 10)
945. small protein blotting - (reply: 8)
946. how stable is alpha mouse HRP? - left on the bench (reply: 2)
947. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
948. Too many bands in my SDS-PAGE - (reply: 5)
949. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
950. Uneven bands in SDS-PAGE - (reply: 5)
951. Can primary antibody be reused ? - (reply: 6)
952. Preparation of tree tissue for western blot - (reply: 1)
953. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
954. direct lysis in Laemmli buffer - (reply: 4)
955. anti-FLAG (M2) cross reaction - (reply: 6)
956. NFkB western blotting - (reply: 3)
957. Mannitol in Lysis Buffer - (reply: 1)
958. how long will a 1M DTT solution will last in -20C - (reply: 5)
959. pellet became insoluble after boiling. - (reply: 4)
960. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)