BioJob
BioBlog
PubAlert
BioTool
BioProduct
BioForum
Protocol
Home
Forum Index (1999-2009) Home
Forum Index (2009-) Home
Live Discussion
Top
:
New Forum Archives (2009-)
: :
SDS-PAGE-and-Western-Blotting
931.
What gel thickness and numebr of combs do you use? - and why??
(reply: 1)
932.
SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION
(reply: 9)
933.
changing colour of front dye in sds-page -
(reply: 10)
934.
small protein blotting -
(reply: 8)
935.
how stable is alpha mouse HRP? - left on the bench
(reply: 2)
936.
Uneven leveling in resolving gel - SDS-PAGE resolving gel woes
(reply: 6)
937.
Too many bands in my SDS-PAGE -
(reply: 5)
938.
Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though.
(reply: 6)
939.
Uneven bands in SDS-PAGE -
(reply: 5)
940.
Can primary antibody be reused ? -
(reply: 6)
941.
Preparation of tree tissue for western blot -
(reply: 1)
942.
Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins
(reply: 3)
943.
direct lysis in Laemmli buffer -
(reply: 4)
944.
anti-FLAG (M2) cross reaction -
(reply: 6)
945.
NFkB western blotting -
(reply: 3)
946.
Mannitol in Lysis Buffer -
(reply: 1)
947.
how long will a 1M DTT solution will last in -20C -
(reply: 5)
948.
pellet became insoluble after boiling. -
(reply: 4)
949.
Pre-Making your own polyacrylamide gels - Short term storage options
(reply: 4)
950.
Ladder but no protein -
(reply: 4)
951.
Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field????
(reply: 1)
952.
my membranes are blue! - silly mistake, what to do?
(reply: 1)
953.
How to solve this western bolt problem? - dumbbell-shape bands
(reply: 2)
954.
Western blotting question - How to strip a mambrane and how many times can it be used
(reply: 7)
955.
Primary Antibody for SPDEF -
(reply: 1)
956.
Detecting Patterns of phosphorylated protein -
(reply: 1)
957.
Sample Diffusion Before Applying Current -
(reply: 4)
958.
IP and western blot -
(reply: 5)
959.
Semi-dry transfer conditions - Volts or Amps
(reply: 1)
960.
Blue Native Gel - denaturing proteins after transfer? -
(reply: 9)
Pages:
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37