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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
931. SDS-PAGE irregular and difuse bands - what's your opinion? (reply: 8)
932. SDS-PAGE Cell lysis problems - (reply: 3)
933. Using Photoshop etc on Western bot images - (reply: 8)
934. Ghost bands in western blot - Western blotting problems (reply: 9)
935. Why immunoblotting of actin coming at higher Molec. Weight - (reply: 1)
936. x-ray films vs direct imaging - Preferred method for western development (reply: 17)
937. proteins stuck at interface between stack and separating gel - (reply: 2)
938. Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins (reply: 16)
939. Lost my proteins ! - (reply: 3)
940. beginner's Questions on Western Blotting - (reply: 4)
941. lost signal - (reply: 4)
942. Using NaN3 to quench HRP conjugated secondary Ab - (reply: 1)
943. White speckles on western blots - Why?? :( (reply: 3)
944. Blocking buffer as antibody dilutent? - (reply: 4)
945. How to prepare colloidal coomassie blue? - ingredients can not dissolve (reply: 4)
946. problem with SDS-PAGE - Sample stuck in the wells of the gel (reply: 5)
947. protein quantification by bradford - (reply: 3)
948. protein extraction from tumor samples containing matrigel? - (reply: 2)
949. western blot - western blot (reply: 17)
950. Amount of protein loaded per well? - (reply: 4)
951. Casp3 - (reply: 2)
952. Anti-FLAG antibody help - Troubleshooting for Sigma anti-FLAG (A8592) (reply: 3)
953. taking protein out of membrane - (reply: 1)
954. prearing gel in advance - (reply: 6)
955. freezing after reducing and boiling - (reply: 10)
956. Invitrogen iBlot - (reply: 6)
957. Even volumes of different samples - (reply: 3)
958. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
959. A single band on WB smaller than expected - (reply: 3)
960. Ponceau staining after primary and secondary? - (reply: 5)

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