Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
931. small protein blotting - (reply: 8)
932. how stable is alpha mouse HRP? - left on the bench (reply: 2)
933. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
934. Too many bands in my SDS-PAGE - (reply: 5)
935. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
936. Uneven bands in SDS-PAGE - (reply: 5)
937. Can primary antibody be reused ? - (reply: 6)
938. Preparation of tree tissue for western blot - (reply: 1)
939. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
940. direct lysis in Laemmli buffer - (reply: 4)
941. anti-FLAG (M2) cross reaction - (reply: 6)
942. NFkB western blotting - (reply: 3)
943. Mannitol in Lysis Buffer - (reply: 1)
944. how long will a 1M DTT solution will last in -20C - (reply: 5)
945. pellet became insoluble after boiling. - (reply: 4)
946. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
947. Ladder but no protein - (reply: 4)
948. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
949. my membranes are blue! - silly mistake, what to do? (reply: 1)
950. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
951. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
952. Primary Antibody for SPDEF - (reply: 1)
953. Detecting Patterns of phosphorylated protein - (reply: 1)
954. Sample Diffusion Before Applying Current - (reply: 4)
955. IP and western blot - (reply: 5)
956. Semi-dry transfer conditions - Volts or Amps (reply: 1)
957. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
958. Destain after PoneauS - (reply: 6)
959. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
960. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37