Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
931. Lost my proteins ! - (reply: 3)
932. beginner's Questions on Western Blotting - (reply: 4)
933. lost signal - (reply: 4)
934. Using NaN3 to quench HRP conjugated secondary Ab - (reply: 1)
935. White speckles on western blots - Why?? :( (reply: 3)
936. Blocking buffer as antibody dilutent? - (reply: 4)
937. How to prepare colloidal coomassie blue? - ingredients can not dissolve (reply: 4)
938. problem with SDS-PAGE - Sample stuck in the wells of the gel (reply: 5)
939. protein quantification by bradford - (reply: 3)
940. protein extraction from tumor samples containing matrigel? - (reply: 2)
941. western blot - western blot (reply: 17)
942. Amount of protein loaded per well? - (reply: 4)
943. Casp3 - (reply: 2)
944. Anti-FLAG antibody help - Troubleshooting for Sigma anti-FLAG (A8592) (reply: 3)
945. taking protein out of membrane - (reply: 1)
946. prearing gel in advance - (reply: 6)
947. freezing after reducing and boiling - (reply: 10)
948. Invitrogen iBlot - (reply: 6)
949. Even volumes of different samples - (reply: 3)
950. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
951. A single band on WB smaller than expected - (reply: 3)
952. Ponceau staining after primary and secondary? - (reply: 5)
953. caspase3 detection - help (reply: 5)
954. Big white spots on PVDF membrane - (reply: 1)
955. High background.... - (reply: 6)
956. WB does not correspond to protein array - (reply: 1)
957. Strange pattern after transfer - (reply: 8)
958. Quantifying Western blots - (reply: 1)
959. Complete transfer, can it be done or not? - (reply: 4)
960. Can add new secondary Ab? - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34