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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
811. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
812. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
813. changing colour of front dye in sds-page - (reply: 10)
814. small protein blotting - (reply: 8)
815. how stable is alpha mouse HRP? - left on the bench (reply: 2)
816. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
817. Too many bands in my SDS-PAGE - (reply: 5)
818. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
819. Uneven bands in SDS-PAGE - (reply: 5)
820. Can primary antibody be reused ? - (reply: 6)
821. Preparation of tree tissue for western blot - (reply: 1)
822. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
823. direct lysis in Laemmli buffer - (reply: 4)
824. anti-FLAG (M2) cross reaction - (reply: 6)
825. NFkB western blotting - (reply: 3)
826. Mannitol in Lysis Buffer - (reply: 1)
827. how long will a 1M DTT solution will last in -20C - (reply: 5)
828. pellet became insoluble after boiling. - (reply: 4)
829. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
830. Ladder but no protein - (reply: 4)
831. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
832. my membranes are blue! - silly mistake, what to do? (reply: 1)
833. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
834. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
835. Primary Antibody for SPDEF - (reply: 1)
836. Detecting Patterns of phosphorylated protein - (reply: 1)
837. Sample Diffusion Before Applying Current - (reply: 4)
838. IP and western blot - (reply: 5)
839. Semi-dry transfer conditions - Volts or Amps (reply: 1)
840. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)

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