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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
811. nonreducing antibody in reducing condition - (reply: 1)
812. High Molecular weight protein separation - (reply: 2)
813. Secreted protein for loading control - (reply: 2)
814. Only borders stained in Silver!!! - (reply: 7)
815. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
816. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
817. bromophenol blue - (reply: 10)
818. Native Page transfer/detection problems - (reply: 1)
819. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
820. nuclear loading control - (reply: 2)
821. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
822. Large protein transfer problem - (reply: 8)
823. overloading GAPDH but nice band of interest - (reply: 2)
824. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
825. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
826. Quantiying bands. - Determination of protein contens (reply: 2)
827. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
828. Strange lane pattern, yes/no pattern - (reply: 3)
829. Native PAGE w. Western protein marker - (reply: 4)
830. protein stuck - (reply: 3)
831. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
832. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
833. changing colour of front dye in sds-page - (reply: 10)
834. small protein blotting - (reply: 8)
835. how stable is alpha mouse HRP? - left on the bench (reply: 2)
836. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
837. Too many bands in my SDS-PAGE - (reply: 5)
838. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
839. Uneven bands in SDS-PAGE - (reply: 5)
840. Can primary antibody be reused ? - (reply: 6)

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