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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
811. Large protein transfer problem - (reply: 8)
812. overloading GAPDH but nice band of interest - (reply: 2)
813. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
814. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
815. Quantiying bands. - Determination of protein contens (reply: 2)
816. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
817. Strange lane pattern, yes/no pattern - (reply: 3)
818. Native PAGE w. Western protein marker - (reply: 4)
819. protein stuck - (reply: 3)
820. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
821. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
822. changing colour of front dye in sds-page - (reply: 10)
823. small protein blotting - (reply: 8)
824. how stable is alpha mouse HRP? - left on the bench (reply: 2)
825. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
826. Too many bands in my SDS-PAGE - (reply: 5)
827. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
828. Uneven bands in SDS-PAGE - (reply: 5)
829. Can primary antibody be reused ? - (reply: 6)
830. Preparation of tree tissue for western blot - (reply: 1)
831. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
832. direct lysis in Laemmli buffer - (reply: 4)
833. anti-FLAG (M2) cross reaction - (reply: 6)
834. NFkB western blotting - (reply: 3)
835. Mannitol in Lysis Buffer - (reply: 1)
836. how long will a 1M DTT solution will last in -20C - (reply: 5)
837. pellet became insoluble after boiling. - (reply: 4)
838. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
839. Ladder but no protein - (reply: 4)
840. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)

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