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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
811. Laminin 5 antibody - (reply: 1)
812. Molecular weight marker - got different pattern! (reply: 13)
813. SDS ladder - which type works better? (reply: 3)
814. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
815. the sensitive amount of protein for western blotting - (reply: 3)
816. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
817. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
818. How to determine final amount of 20 ng protein per WB well - (reply: 8)
819. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
820. nonreducing antibody in reducing condition - (reply: 1)
821. High Molecular weight protein separation - (reply: 2)
822. Secreted protein for loading control - (reply: 2)
823. Only borders stained in Silver!!! - (reply: 7)
824. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
825. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
826. bromophenol blue - (reply: 10)
827. Native Page transfer/detection problems - (reply: 1)
828. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
829. nuclear loading control - (reply: 2)
830. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
831. Large protein transfer problem - (reply: 8)
832. overloading GAPDH but nice band of interest - (reply: 2)
833. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
834. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
835. Quantiying bands. - Determination of protein contens (reply: 2)
836. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
837. Strange lane pattern, yes/no pattern - (reply: 3)
838. Native PAGE w. Western protein marker - (reply: 4)
839. protein stuck - (reply: 3)
840. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)

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