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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
811. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
812. Western with conjugated antibody? - (reply: 2)
813. Protein bands on the bottom of the gel - (reply: 5)
814. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
815. Tube gel IEF problems - Polymerization... (reply: 2)
816. Cannot detect GFP using GFP antibody - (reply: 10)
817. Who can help me with my stupid mistake - wrong first antibody (reply: 3)
818. Acrylamide/bis stock expiration? - (reply: 2)
819. small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
820. Incubation with washing buffer only? - (reply: 4)
821. Ponceau Re-staining - (reply: 1)
822. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
823. color of 5 times SDS loading dye - (reply: 1)
824. Protein concentration and - (reply: 3)
825. Running bacterias as control on SDS-PAGE - should I see anything without breaking them? (reply: 3)
826. Multiple antibodies give no signal - (reply: 10)
827. Problem with protein samples - No bands detected at all on gel (reply: 2)
828. using cell lysate to run native gel? - (reply: 1)
829. Bradford Query - (reply: 5)
830. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
831. About GFP- tagged proteins in western blot - (reply: 1)
832. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
833. PVP-40 for blocking? - Instead of milk (reply: 2)
834. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
835. How to pase my Co-IP over the weekend? - (reply: 1)
836. Is beta-actin a good cyto vs. nuc control? - (reply: 2)
837. Protein Sample Preparation - Western Bloting (reply: 1)
838. No signal at all - (reply: 16)
839. Centrifuge after lysing - (reply: 5)
840. Isse with "Smiles" on gel - Lanes with Octyl-POE run strangely (reply: 1)

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