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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
811. Ponceau staining - Appearence of bands (reply: 1)
812. Detecting phosphorylated Erk1/2 - (reply: 6)
813. Detecting BDNF with WB... - troubleshooting... (reply: 2)
814. western blot using culture media - (reply: 4)
815. Re-inserting marker into scanned WB - (reply: 1)
816. pre stained marker issues - (reply: 2)
817. Functions of BSA and Tween 20 in western blotting - (reply: 2)
818. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
819. Western with conjugated antibody? - (reply: 2)
820. Protein bands on the bottom of the gel - (reply: 5)
821. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
822. Tube gel IEF problems - Polymerization... (reply: 2)
823. Cannot detect GFP using GFP antibody - (reply: 10)
824. Who can help me with my stupid mistake - wrong first antibody (reply: 3)
825. Acrylamide/bis stock expiration? - (reply: 2)
826. small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
827. Incubation with washing buffer only? - (reply: 4)
828. Ponceau Re-staining - (reply: 1)
829. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
830. color of 5 times SDS loading dye - (reply: 1)
831. Protein concentration and - (reply: 3)
832. Running bacterias as control on SDS-PAGE - should I see anything without breaking them? (reply: 3)
833. Multiple antibodies give no signal - (reply: 10)
834. Problem with protein samples - No bands detected at all on gel (reply: 2)
835. using cell lysate to run native gel? - (reply: 1)
836. Bradford Query - (reply: 5)
837. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
838. About GFP- tagged proteins in western blot - (reply: 1)
839. SDS-PAGE after ammonium sulfate precipitation - (reply: 3)
840. PVP-40 for blocking? - Instead of milk (reply: 2)

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