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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
811. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
812. Protein Expression in stationary phase - (reply: 11)
813. Protein Smearing - (reply: 2)
814. Western blot for muscle - (reply: 2)
815. Nupage Bis/Tris Current Voltage problem - Current is so high! (reply: 12)
816. Identify the band in western blot - (reply: 5)
817. What kind of post-translational modification do you think are these? - (reply: 2)
818. Need help with Caspase WB - (reply: 2)
819. to keep sds-pa gels - (reply: 2)
820. Overexpressed protein not detected - Oh, the frustration... (reply: 1)
821. Ponceau staining - Appearence of bands (reply: 1)
822. Detecting phosphorylated Erk1/2 - (reply: 6)
823. Detecting BDNF with WB... - troubleshooting... (reply: 2)
824. western blot using culture media - (reply: 4)
825. Re-inserting marker into scanned WB - (reply: 1)
826. pre stained marker issues - (reply: 2)
827. Functions of BSA and Tween 20 in western blotting - (reply: 2)
828. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
829. Western with conjugated antibody? - (reply: 2)
830. Protein bands on the bottom of the gel - (reply: 5)
831. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
832. Tube gel IEF problems - Polymerization... (reply: 2)
833. Cannot detect GFP using GFP antibody - (reply: 10)
834. Who can help me with my stupid mistake - wrong first antibody (reply: 3)
835. Acrylamide/bis stock expiration? - (reply: 2)
836. small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
837. Incubation with washing buffer only? - (reply: 4)
838. Ponceau Re-staining - (reply: 1)
839. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
840. color of 5 times SDS loading dye - (reply: 1)

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