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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. 37kb and 7kb protein in western blot - (reply: 24)
62. Dot blot/Western background signal problem - (reply: 1)
63. Blocking and Washing membrane - (reply: 3)
64. detection of a protein band of protein complex - (reply: 3)
65. Cell lysis - (reply: 1)
66. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
67. Western blot detects exogenous protein but not endogenous - (reply: 2)
68. White bands in western blotting - (reply: 4)
69. Question with the molecular weight of my protein - (reply: 1)
70. Possible Protein Aggregation Riddle - (reply: 3)
71. How to obtain a control positive for my WB - (reply: 3)
72. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
73. Antibody not binding to low MW targets - (reply: 4)
74. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
75. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
76. Question on loss of signal between samples in western blot - (reply: 4)
77. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
78. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
79. Antibody Issues - (reply: 6)
80. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
81. Antibody-Biotinylation, detection via SDS-Gel - (reply: 10)
82. Is it possible to "over-block" a membrane? - (reply: 5)
83. Troubleshooting for BN PAGE - (reply: 10)
84. SDS PAGE Destaining - (reply: 7)
85. Layer of water above separating gel mixes into gel - (reply: 2)
86. PCR & Western Blot sample preperation - (reply: 2)
87. SDS page - (reply: 8)
88. Two-night primary antibody incubation? - (reply: 2)
89. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
90. A question about Gelanalyzer - (reply: 8)

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