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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. patchy/holey bands after transfer, invasive bubbles? - (reply: 5)
62. Western not working - (reply: 4)
63. fused bands of beta-actin in western blot - (reply: 2)
64. Suggestions - Nice Western Blot Image from Office Scanner? - (reply: 7)
65. Undergrad "activated" a NS membrane with 100% MeOH... Any hope? - (reply: 5)
66. Problem with GFP detection in western - (reply: 6)
67. Sudden Trouble with PVDF Transfer - (reply: 6)
68. Equal Loading Confusion - (reply: 6)
69. Wrong protein size on western blot - (reply: 10)
70. How to know if the signal of western blot is saturated ? - (reply: 4)
71. Scotch-Brite versus other foam pad for wet transfer? - (reply: 3)
72. 5x SDS lysis buffer - (reply: 5)
73. Protein measurement of Laemmli samples - (reply: 2)
74. SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
75. Uneven banding - Western blot troubleshooting - (reply: 4)
76. Western Blot with fixed cells - (reply: 5)
77. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
78. Primary antibody - (reply: 1)
79. Native-PAGE, help! - (reply: 3)
80. Trouble with purified recombinant protein bands - (reply: 3)
81. Stain in electrophoresis is going from + to - - (reply: 3)
82. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
83. Extraction of Transmembrane Proteins - (reply: 1)
84. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
85. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
86. Protein amount to load for publication figure? - (reply: 4)
87. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
88. What is different between Native protein and Denatured protein samples. - (reply: 4)
89. 3 extra bands show up on my western after an IP - (reply: 2)
90. IgG heavy chain from IP - (reply: 1)

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