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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. Antibody not binding to low MW targets - (reply: 4)
62. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
63. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
64. Question on loss of signal between samples in western blot - (reply: 4)
65. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
66. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
67. Antibody Issues - (reply: 6)
68. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
69. Antibody-Biotinylation, detection via SDS-Gel - (reply: 10)
70. Is it possible to "over-block" a membrane? - (reply: 5)
71. Troubleshooting for BN PAGE - (reply: 10)
72. SDS PAGE Destaining - (reply: 7)
73. Layer of water above separating gel mixes into gel - (reply: 2)
74. PCR & Western Blot sample preperation - (reply: 2)
75. SDS page - (reply: 8)
76. Two-night primary antibody incubation? - (reply: 2)
77. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
78. A question about Gelanalyzer - (reply: 8)
79. storing membrane - (reply: 2)
80. band obove the ladder - (reply: 3)
81. DNA SDS-PAGE electrophoresis - (reply: 6)
82. Axin1 Co-IP - (reply: 2)
83. Glycine vs. tricine - (reply: 1)
84. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
85. SDS-PAGE power supply issues - (reply: 3)
86. Result in the lab is not the same as published - (reply: 3)
87. PKC theta - (reply: 3)
88. reason for curved bands? - (reply: 2)
89. Protein bands visible on unstained SDS-PAGE - (reply: 1)
90. Inconsistent stripping - (reply: 3)

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