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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
62. Question on loss of signal between samples in western blot - (reply: 4)
63. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
64. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
65. Antibody Issues - (reply: 6)
66. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
67. Antibody-Biotinylation, detection via SDS-Gel - (reply: 10)
68. Is it possible to "over-block" a membrane? - (reply: 5)
69. Troubleshooting for BN PAGE - (reply: 10)
70. SDS PAGE Destaining - (reply: 7)
71. Layer of water above separating gel mixes into gel - (reply: 2)
72. PCR & Western Blot sample preperation - (reply: 2)
73. SDS page - (reply: 8)
74. Two-night primary antibody incubation? - (reply: 2)
75. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
76. A question about Gelanalyzer - (reply: 8)
77. storing membrane - (reply: 2)
78. band obove the ladder - (reply: 3)
79. DNA SDS-PAGE electrophoresis - (reply: 6)
80. Axin1 Co-IP - (reply: 2)
81. Glycine vs. tricine - (reply: 1)
82. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
83. SDS-PAGE power supply issues - (reply: 3)
84. Result in the lab is not the same as published - (reply: 3)
85. PKC theta - (reply: 3)
86. reason for curved bands? - (reply: 2)
87. Protein bands visible on unstained SDS-PAGE - (reply: 1)
88. Inconsistent stripping - (reply: 3)
89. After Co Ip can Iuse SDS PAGE - (reply: 1)
90. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)

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