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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. Western blot with smeared bands - (reply: 3)
62. SDS PAGE sample preparation question, and discussion? - (reply: 3)
63. too much heating LDS-sample buffer - (reply: 3)
64. problem of cell lysate - (reply: 3)
65. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
66. Blocking agent in western blotting of histone proteins - (reply: 1)
67. Best western loading control for comparing different cell lines - (reply: 2)
68. western blotting running problem? - (reply: 2)
69. Keeping apoptotic/detached cells for WB? - (reply: 3)
70. Advice with WB please - (reply: 3)
71. Western Blot Wet Transfer Conditions - (reply: 10)
72. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
73. Western Blot and Inmunofluorescence contradictions - (reply: 12)
74. Nanog double band in WB - (reply: 1)
75. My beta actin bands are changing - (reply: 6)
76. ECL Substrate Choices - (reply: 2)
77. Why does my gel have streaks instead of bands? - (reply: 2)
78. Left ECL kit at room temp. Is it ruined? - (reply: 2)
79. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
80. Native PAGE problem - not entering gel enough - (reply: 9)
81. film saturation in immunoblots - (reply: 1)
82. Problems with wells on SDS PAGE gels - (reply: 3)
83. Protein is present, but not detected by antibody - (reply: 4)
84. secondary antibody creation - (reply: 1)
85. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
86. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
87. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
88. Low signal, weird background stains on blot - (reply: 5)
89. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
90. Incomplete Blotting and staining of NC membrane - (reply: 7)

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