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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. Protein not migrating through gel - (reply: 9)
62. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
63. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
64. Extraction of bands from SDS-PAGE - (reply: 2)
65. Dumbbell-shaped bands on the WB - (reply: 3)
66. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
67. Deformed bands in gel - (reply: 8)
68. non specific immunoblot - (reply: 2)
69. Protein stuck in the origin of resolving gel - (reply: 12)
70. detection of small peptide by the SDS-PAGE gel - (reply: 1)
71. Help in western blotting GAPDH bands are not even - (reply: 1)
72. 37kb and 7kb protein in western blot - (reply: 24)
73. Dot blot/Western background signal problem - (reply: 1)
74. Blocking and Washing membrane - (reply: 3)
75. detection of a protein band of protein complex - (reply: 3)
76. Cell lysis - (reply: 1)
77. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
78. Western blot detects exogenous protein but not endogenous - (reply: 2)
79. White bands in western blotting - (reply: 4)
80. Question with the molecular weight of my protein - (reply: 1)
81. Possible Protein Aggregation Riddle - (reply: 3)
82. How to obtain a control positive for my WB - (reply: 3)
83. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
84. Antibody not binding to low MW targets - (reply: 4)
85. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
86. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
87. Question on loss of signal between samples in western blot - (reply: 4)
88. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
89. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
90. Antibody Issues - (reply: 6)

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