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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
62. Blocking agent in western blotting of histone proteins - (reply: 1)
63. Best western loading control for comparing different cell lines - (reply: 2)
64. western blotting running problem? - (reply: 2)
65. Keeping apoptotic/detached cells for WB? - (reply: 3)
66. Advice with WB please - (reply: 3)
67. Western Blot Wet Transfer Conditions - (reply: 10)
68. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
69. Western Blot and Inmunofluorescence contradictions - (reply: 12)
70. Nanog double band in WB - (reply: 1)
71. My beta actin bands are changing - (reply: 6)
72. ECL Substrate Choices - (reply: 2)
73. Why does my gel have streaks instead of bands? - (reply: 2)
74. Left ECL kit at room temp. Is it ruined? - (reply: 2)
75. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
76. Native PAGE problem - not entering gel enough - (reply: 9)
77. film saturation in immunoblots - (reply: 1)
78. Problems with wells on SDS PAGE gels - (reply: 3)
79. Protein is present, but not detected by antibody - (reply: 4)
80. secondary antibody creation - (reply: 1)
81. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
82. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
83. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
84. Low signal, weird background stains on blot - (reply: 5)
85. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
86. Incomplete Blotting and staining of NC membrane - (reply: 7)
87. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
88. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
89. Help, protein ladder didn`t separate - (reply: 3)
90. Determining phosphorylation status of a protein by transfection - (reply: 5)

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