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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. Rapidly decomposing HRP substrate - (reply: 2)
62. protein size in SDS-PAGE - (reply: 4)
63. Protein not migrating through gel - (reply: 9)
64. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
65. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
66. Extraction of bands from SDS-PAGE - (reply: 2)
67. Dumbbell-shaped bands on the WB - (reply: 3)
68. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
69. Deformed bands in gel - (reply: 8)
70. non specific immunoblot - (reply: 2)
71. Protein stuck in the origin of resolving gel - (reply: 12)
72. detection of small peptide by the SDS-PAGE gel - (reply: 1)
73. Help in western blotting GAPDH bands are not even - (reply: 1)
74. 37kb and 7kb protein in western blot - (reply: 24)
75. Dot blot/Western background signal problem - (reply: 1)
76. Blocking and Washing membrane - (reply: 3)
77. detection of a protein band of protein complex - (reply: 3)
78. Cell lysis - (reply: 1)
79. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
80. Western blot detects exogenous protein but not endogenous - (reply: 2)
81. White bands in western blotting - (reply: 4)
82. Question with the molecular weight of my protein - (reply: 1)
83. Possible Protein Aggregation Riddle - (reply: 3)
84. How to obtain a control positive for my WB - (reply: 3)
85. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
86. Antibody not binding to low MW targets - (reply: 4)
87. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
88. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
89. Question on loss of signal between samples in western blot - (reply: 4)
90. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)

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