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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. Question on loss of signal between samples in western blot - (reply: 4)
62. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
63. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
64. Antibody Issues - (reply: 6)
65. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
66. Antibody-Biotinylation, detection via SDS-Gel - (reply: 10)
67. Is it possible to "over-block" a membrane? - (reply: 5)
68. Troubleshooting for BN PAGE - (reply: 10)
69. SDS PAGE Destaining - (reply: 7)
70. Layer of water above separating gel mixes into gel - (reply: 2)
71. PCR & Western Blot sample preperation - (reply: 2)
72. SDS page - (reply: 8)
73. Two-night primary antibody incubation? - (reply: 2)
74. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
75. A question about Gelanalyzer - (reply: 8)
76. storing membrane - (reply: 2)
77. band obove the ladder - (reply: 3)
78. DNA SDS-PAGE electrophoresis - (reply: 6)
79. Axin1 Co-IP - (reply: 2)
80. Glycine vs. tricine - (reply: 1)
81. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
82. SDS-PAGE power supply issues - (reply: 3)
83. Result in the lab is not the same as published - (reply: 3)
84. PKC theta - (reply: 3)
85. reason for curved bands? - (reply: 2)
86. Protein bands visible on unstained SDS-PAGE - (reply: 1)
87. Inconsistent stripping - (reply: 3)
88. After Co Ip can Iuse SDS PAGE - (reply: 1)
89. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
90. BCIP/NBT developed blot disappearing - (reply: 1)

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