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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. non specific immunoblot - (reply: 2)
62. Protein stuck in the origin of resolving gel - (reply: 12)
63. detection of small peptide by the SDS-PAGE gel - (reply: 1)
64. Help in western blotting GAPDH bands are not even - (reply: 1)
65. 37kb and 7kb protein in western blot - (reply: 24)
66. Dot blot/Western background signal problem - (reply: 1)
67. Blocking and Washing membrane - (reply: 3)
68. detection of a protein band of protein complex - (reply: 3)
69. Cell lysis - (reply: 1)
70. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
71. Western blot detects exogenous protein but not endogenous - (reply: 2)
72. White bands in western blotting - (reply: 4)
73. Question with the molecular weight of my protein - (reply: 1)
74. Possible Protein Aggregation Riddle - (reply: 3)
75. How to obtain a control positive for my WB - (reply: 3)
76. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
77. Antibody not binding to low MW targets - (reply: 4)
78. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
79. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
80. Question on loss of signal between samples in western blot - (reply: 4)
81. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
82. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
83. Antibody Issues - (reply: 6)
84. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
85. Antibody-Biotinylation, detection via SDS-Gel - (reply: 10)
86. Is it possible to "over-block" a membrane? - (reply: 5)
87. Troubleshooting for BN PAGE - (reply: 10)
88. SDS PAGE Destaining - (reply: 7)
89. Layer of water above separating gel mixes into gel - (reply: 2)
90. PCR & Western Blot sample preperation - (reply: 2)

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