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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
62. Antibody not binding to low MW targets - (reply: 4)
63. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
64. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
65. Question on loss of signal between samples in western blot - (reply: 4)
66. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
67. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
68. Antibody Issues - (reply: 6)
69. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
70. Antibody-Biotinylation, detection via SDS-Gel - (reply: 10)
71. Is it possible to "over-block" a membrane? - (reply: 5)
72. Troubleshooting for BN PAGE - (reply: 10)
73. SDS PAGE Destaining - (reply: 7)
74. Layer of water above separating gel mixes into gel - (reply: 2)
75. PCR & Western Blot sample preperation - (reply: 2)
76. SDS page - (reply: 8)
77. Two-night primary antibody incubation? - (reply: 2)
78. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
79. A question about Gelanalyzer - (reply: 8)
80. storing membrane - (reply: 2)
81. band obove the ladder - (reply: 3)
82. DNA SDS-PAGE electrophoresis - (reply: 6)
83. Axin1 Co-IP - (reply: 2)
84. Glycine vs. tricine - (reply: 1)
85. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
86. SDS-PAGE power supply issues - (reply: 3)
87. Result in the lab is not the same as published - (reply: 3)
88. PKC theta - (reply: 3)
89. reason for curved bands? - (reply: 2)
90. Protein bands visible on unstained SDS-PAGE - (reply: 1)

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