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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. How to obtain a control positive for my WB - (reply: 3)
62. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
63. Antibody not binding to low MW targets - (reply: 4)
64. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
65. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
66. Question on loss of signal between samples in western blot - (reply: 4)
67. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
68. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
69. Antibody Issues - (reply: 6)
70. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
71. Antibody-Biotinylation, detection via SDS-Gel - (reply: 10)
72. Is it possible to "over-block" a membrane? - (reply: 5)
73. Troubleshooting for BN PAGE - (reply: 10)
74. SDS PAGE Destaining - (reply: 7)
75. Layer of water above separating gel mixes into gel - (reply: 2)
76. PCR & Western Blot sample preperation - (reply: 2)
77. SDS page - (reply: 8)
78. Two-night primary antibody incubation? - (reply: 2)
79. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
80. A question about Gelanalyzer - (reply: 8)
81. storing membrane - (reply: 2)
82. band obove the ladder - (reply: 3)
83. DNA SDS-PAGE electrophoresis - (reply: 6)
84. Axin1 Co-IP - (reply: 2)
85. Glycine vs. tricine - (reply: 1)
86. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
87. SDS-PAGE power supply issues - (reply: 3)
88. Result in the lab is not the same as published - (reply: 3)
89. PKC theta - (reply: 3)
90. reason for curved bands? - (reply: 2)

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