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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. SDS PAGE gels - (reply: 5)
62. chemi doc mp from biorad image quality - (reply: 2)
63. Western Blot v ELISA for Different Samples? - (reply: 1)
64. Premixing gel solutions - (reply: 2)
65. getting negative protein measurements - (reply: 3)
66. Peculiar background problem with MPM-2 antibodies - (reply: 2)
67. Stripping the membrane for LiCor - (reply: 1)
68. 17 kDa ideal transfer voltage - PVDF - (reply: 4)
69. Lysis buffer - (reply: 2)
70. SDS Page and WB: Wrong size bands - (reply: 2)
71. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
72. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
73. How to avoid gel breakage after electrophoresis - (reply: 5)
74. What is an Input in SDS-PAGE? - (reply: 1)
75. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
76. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
77. Good loading control for native gels? - (reply: 1)
78. Please help with odd bands and smears on Western blot - (reply: 4)
79. How to load equal amount of protein without using spectramax - (reply: 4)
80. Western blot membrane imaging - (reply: 7)
81. Detection of T7 RNA polymerase by WB - (reply: 6)
82. Shift in the position of band onto the western blot - (reply: 3)
83. Ghost and inverse bands and high background. Help! - (reply: 3)
84. No resolving of protein/bands below 50kDa :-s - (reply: 5)
85. Problems with wet transfer blot - (reply: 2)
86. 5% BSA blocking buffer - (reply: 3)
87. Storage of Membrane before addition of primary antibody - (reply: 3)
88. "Mickey Mouse" bands - (reply: 5)
89. SDS-PAGE : when the transfer stops - (reply: 1)
90. confusing Western results - (reply: 5)

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