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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. ECL Substrate Choices - (reply: 2)
62. Why does my gel have streaks instead of bands? - (reply: 2)
63. Left ECL kit at room temp. Is it ruined? - (reply: 2)
64. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
65. Native PAGE problem - not entering gel enough - (reply: 9)
66. film saturation in immunoblots - (reply: 1)
67. Problems with wells on SDS PAGE gels - (reply: 3)
68. Protein is present, but not detected by antibody - (reply: 4)
69. secondary antibody creation - (reply: 1)
70. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
71. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
72. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
73. Low signal, weird background stains on blot - (reply: 5)
74. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
75. Incomplete Blotting and staining of NC membrane - (reply: 7)
76. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
77. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
78. Help, protein ladder didn`t separate - (reply: 3)
79. Determining phosphorylation status of a protein by transfection - (reply: 5)
80. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
81. Problem with Denative lysis - (reply: 1)
82. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
83. Polyclonal antibody precipitating - (reply: 1)
84. manual 2D electrophoresis - (reply: 8)
85. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
86. sample sonication before loading - (reply: 6)
87. transfer of low molecular weight protein - (reply: 3)
88. How is the mass detected via SDS-PAGE? - (reply: 3)
89. Water in beads during boiling - (reply: 3)
90. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)

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