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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. Problems with histones in SDS-PAGE - (reply: 1)
62. Stripping problem - (reply: 5)
63. Problems of beta-actin - (reply: 1)
64. Problem with 15% SDS page gel during electrophoresis - (reply: 7)
65. Problems of western signal - (reply: 5)
66. How many nitrocellulose blots can be safely stripped and incubated in primary/se - (reply: 1)
67. 2x laemmli buffer - (reply: 4)
68. Relative humidity in the electrophoresis lab - (reply: 3)
69. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
70. how can i explain the different pattern from my labmate's one - (reply: 8)
71. Ladder and proteins do not migrate into stacking gel - (reply: 5)
72. Western Blot: Unequal Sample Migration - (reply: 4)
73. Western blot with smeared bands - (reply: 3)
74. SDS PAGE sample preparation question, and discussion? - (reply: 3)
75. too much heating LDS-sample buffer - (reply: 3)
76. problem of cell lysate - (reply: 3)
77. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
78. Blocking agent in western blotting of histone proteins - (reply: 1)
79. Best western loading control for comparing different cell lines - (reply: 2)
80. western blotting running problem? - (reply: 2)
81. Keeping apoptotic/detached cells for WB? - (reply: 3)
82. Advice with WB please - (reply: 3)
83. Western Blot Wet Transfer Conditions - (reply: 10)
84. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
85. Western Blot and Inmunofluorescence contradictions - (reply: 12)
86. Nanog double band in WB - (reply: 1)
87. My beta actin bands are changing - (reply: 6)
88. ECL Substrate Choices - (reply: 2)
89. Why does my gel have streaks instead of bands? - (reply: 2)
90. Left ECL kit at room temp. Is it ruined? - (reply: 2)

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