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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
62. Help, protein ladder didn`t separate - (reply: 3)
63. Determining phosphorylation status of a protein by transfection - (reply: 5)
64. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
65. Problem with Denative lysis - (reply: 1)
66. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
67. Polyclonal antibody precipitating - (reply: 1)
68. manual 2D electrophoresis - (reply: 8)
69. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
70. sample sonication before loading - (reply: 6)
71. transfer of low molecular weight protein - (reply: 3)
72. How is the mass detected via SDS-PAGE? - (reply: 3)
73. Water in beads during boiling - (reply: 3)
74. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
75. Runing problems - (reply: 18)
76. Western Blotting of Amyloid Beta - (reply: 1)
77. 10x Laemmli buffer - (reply: 5)
78. Need help translating my western blot protocol into every language - (reply: 3)
79. Semi-dry system for NuPage with MES buffer - (reply: 4)
80. Recommend anti-rat HRP secondary antibody - (reply: 2)
81. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
82. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)
83. Why Squeezing of lanes occur at bottom in PAGE - (reply: 6)
84. Western blot to CD44? - (reply: 3)
85. Amount of EDTA - (reply: 4)
86. SDS Apparatus for northern - (reply: 1)
87. Prey protein band bleeds, IgG band normal? - (reply: 3)
88. Coomassie gel after semi-dry transfer - normal like this? - (reply: 7)
89. His-Probe - (reply: 2)
90. Problems with western membrane stripping - (reply: 2)

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