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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
61. secondary antibody creation - (reply: 1)
62. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
63. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
64. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
65. Low signal, weird background stains on blot - (reply: 5)
66. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
67. Incomplete Blotting and staining of NC membrane - (reply: 7)
68. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
69. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
70. Help, protein ladder didn`t separate - (reply: 3)
71. Determining phosphorylation status of a protein by transfection - (reply: 5)
72. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
73. Problem with Denative lysis - (reply: 1)
74. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
75. Polyclonal antibody precipitating - (reply: 1)
76. manual 2D electrophoresis - (reply: 8)
77. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
78. sample sonication before loading - (reply: 6)
79. transfer of low molecular weight protein - (reply: 3)
80. How is the mass detected via SDS-PAGE? - (reply: 3)
81. Water in beads during boiling - (reply: 3)
82. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
83. Runing problems - (reply: 18)
84. Western Blotting of Amyloid Beta - (reply: 1)
85. 10x Laemmli buffer - (reply: 5)
86. Need help translating my western blot protocol into every language - (reply: 3)
87. Semi-dry system for NuPage with MES buffer - (reply: 4)
88. Recommend anti-rat HRP secondary antibody - (reply: 2)
89. Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
90. Rewetting the dry PVDF after secondary antibody with MetOH or not? - (reply: 1)

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