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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
271. Uneven lane widths during western blots - (reply: 10)
272. Using human serum as first antibody in Western/ELISA - (reply: 3)
273. Strip Western Blotting Troubleshooting - (reply: 2)
274. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
275. Phospho-ATF-2 coming up different sizes - (reply: 1)
276. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
277. A possible protocol for western blotting troubleshooting? - (reply: 1)
278. Vertical Vs horizontal electrophoresis - (reply: 3)
279. Reprobing with the same antibody - (reply: 3)
280. SDS-PAGE problem with staining yellow background - (reply: 3)
281. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
282. Western blot narrow bands - (reply: 1)
283. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
284. Western Blot Antibody Epitope - (reply: 1)
285. Western blot band anaylisis - (reply: 3)
286. protein aggregation (?) in gelatin zymography - (reply: 1)
287. Use of wrong TBS concentration - (reply: 1)
288. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
289. SDS-PAGE staining for digestion assay - (reply: 1)
290. Can I improve my primary antibody? - (reply: 2)
291. got band in control positive but not in sample... - (reply: 2)
292. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
293. Problems with IPs - (reply: 2)
294. Band is at the wrong size, but same for all antibodies - (reply: 6)
295. Problems with western blot detection - (reply: 1)
296. Methods of concentrating total protein extracts - (reply: 2)
297. Using serum as the sample in Western blot - (reply: 3)
298. Puzzeling western blot results, missing band - (reply: 3)
299. Secondary AB blocking - (reply: 1)
300. western blood immunodetection Ab - (reply: 1)

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