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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
271. Non-specific bands and fuzzy bands - (reply: 1)
272. protein showing up around 50 kda rather than 20 kda - (reply: 2)
273. Help for western blot wet transfer blurry bands - (reply: 2)
274. anti-BRCA1 antibody specificity issues - (reply: 2)
275. Concentrating a diluted protein and protein determination - (reply: 4)
276. Blurry bands ... - (reply: 12)
277. Dot blotting problems - (reply: 3)
278. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
279. Uneven lane widths during western blots - (reply: 10)
280. Using human serum as first antibody in Western/ELISA - (reply: 3)
281. Strip Western Blotting Troubleshooting - (reply: 2)
282. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
283. Phospho-ATF-2 coming up different sizes - (reply: 1)
284. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
285. A possible protocol for western blotting troubleshooting? - (reply: 1)
286. Vertical Vs horizontal electrophoresis - (reply: 3)
287. Reprobing with the same antibody - (reply: 3)
288. SDS-PAGE problem with staining yellow background - (reply: 3)
289. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
290. Western blot narrow bands - (reply: 1)
291. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
292. Western Blot Antibody Epitope - (reply: 1)
293. Western blot band anaylisis - (reply: 3)
294. protein aggregation (?) in gelatin zymography - (reply: 1)
295. Use of wrong TBS concentration - (reply: 1)
296. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
297. SDS-PAGE staining for digestion assay - (reply: 1)
298. Can I improve my primary antibody? - (reply: 2)
299. got band in control positive but not in sample... - (reply: 2)
300. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)

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