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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
271. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
272. Changes to housekeeping loading controls after drug treatment - (reply: 2)
273. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
274. Multiple bands on blot with one antibody - (reply: 1)
275. sedimentation of cytosolic proteins - (reply: 2)
276. Determining pI of a protein - (reply: 3)
277. Non-Reduced Western Blot - (reply: 5)
278. Incomplete transfer - (reply: 5)
279. can coommassie stained gel be western blot - (reply: 5)
280. Wet transfer: western blot - (reply: 3)
281. Reprobing a PVDF membrane which has gone dry - (reply: 1)
282. Non-specific bands and fuzzy bands - (reply: 1)
283. protein showing up around 50 kda rather than 20 kda - (reply: 2)
284. Help for western blot wet transfer blurry bands - (reply: 2)
285. anti-BRCA1 antibody specificity issues - (reply: 2)
286. Concentrating a diluted protein and protein determination - (reply: 4)
287. Blurry bands ... - (reply: 12)
288. Dot blotting problems - (reply: 3)
289. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
290. Uneven lane widths during western blots - (reply: 10)
291. Using human serum as first antibody in Western/ELISA - (reply: 3)
292. Strip Western Blotting Troubleshooting - (reply: 2)
293. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
294. Phospho-ATF-2 coming up different sizes - (reply: 1)
295. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
296. A possible protocol for western blotting troubleshooting? - (reply: 1)
297. Vertical Vs horizontal electrophoresis - (reply: 3)
298. Reprobing with the same antibody - (reply: 3)
299. SDS-PAGE problem with staining yellow background - (reply: 3)
300. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)

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