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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
271. Peculiar background problem with MPM-2 antibodies - (reply: 2)
272. Stripping the membrane for LiCor - (reply: 1)
273. 17 kDa ideal transfer voltage - PVDF - (reply: 4)
274. Lysis buffer - (reply: 2)
275. SDS Page and WB: Wrong size bands - (reply: 2)
276. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
277. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
278. How to avoid gel breakage after electrophoresis - (reply: 5)
279. What is an Input in SDS-PAGE? - (reply: 1)
280. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
281. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
282. Good loading control for native gels? - (reply: 1)
283. Please help with odd bands and smears on Western blot - (reply: 4)
284. How to load equal amount of protein without using spectramax - (reply: 4)
285. Western blot membrane imaging - (reply: 7)
286. Detection of T7 RNA polymerase by WB - (reply: 6)
287. Shift in the position of band onto the western blot - (reply: 3)
288. Ghost and inverse bands and high background. Help! - (reply: 3)
289. No resolving of protein/bands below 50kDa :-s - (reply: 5)
290. Problems with wet transfer blot - (reply: 2)
291. 5% BSA blocking buffer - (reply: 3)
292. Storage of Membrane before addition of primary antibody - (reply: 3)
293. "Mickey Mouse" bands - (reply: 5)
294. SDS-PAGE : when the transfer stops - (reply: 1)
295. confusing Western results - (reply: 5)
296. aditional band on WB - (reply: 1)
297. Transferring >150 kDA proteins conditions - (reply: 3)
298. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
299. TAU gel electrophoresis of histones - (reply: 4)
300. Bands dark on sides and light in middle - (reply: 7)

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