Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
271. 17 kDa ideal transfer voltage - PVDF - (reply: 4)
272. Lysis buffer - (reply: 2)
273. SDS Page and WB: Wrong size bands - (reply: 2)
274. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
275. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
276. How to avoid gel breakage after electrophoresis - (reply: 5)
277. What is an Input in SDS-PAGE? - (reply: 1)
278. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
279. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
280. Good loading control for native gels? - (reply: 1)
281. Please help with odd bands and smears on Western blot - (reply: 4)
282. How to load equal amount of protein without using spectramax - (reply: 4)
283. Western blot membrane imaging - (reply: 7)
284. Detection of T7 RNA polymerase by WB - (reply: 6)
285. Shift in the position of band onto the western blot - (reply: 3)
286. Ghost and inverse bands and high background. Help! - (reply: 3)
287. No resolving of protein/bands below 50kDa :-s - (reply: 5)
288. Problems with wet transfer blot - (reply: 2)
289. 5% BSA blocking buffer - (reply: 3)
290. Storage of Membrane before addition of primary antibody - (reply: 3)
291. "Mickey Mouse" bands - (reply: 5)
292. SDS-PAGE : when the transfer stops - (reply: 1)
293. confusing Western results - (reply: 5)
294. aditional band on WB - (reply: 1)
295. Transferring >150 kDA proteins conditions - (reply: 3)
296. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
297. TAU gel electrophoresis of histones - (reply: 4)
298. Bands dark on sides and light in middle - (reply: 7)
299. Addition of sample loading buffer, before or after heating - (reply: 5)
300. question about protein expression normalization. - (reply: 6)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36