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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
271. question about protein expression normalization. - (reply: 6)
272. Protein bands not migrating - (reply: 3)
273. Help with high background (have tried most trouble shooting) - (reply: 8)
274. Tumor protein precipitation at denaturing step? - (reply: 1)
275. How to prepare blood serum for western blot? - (reply: 3)
276. TNF alpha and IL 1b Western Blot - (reply: 1)
277. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
278. SDS-PAGE trouble with protein concentration - (reply: 2)
279. Acrylamid - (reply: 5)
280. Detecting doublet bands in Western for eGFP control - (reply: 1)
281. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
282. Protein Size 50 kDa higher than expected - (reply: 3)
283. Acid urea gel electrophoresis (AUGE) - (reply: 7)
284. Phospho-protein vs total protein Western blot - (reply: 5)
285. storing the loaded gel before running it - (reply: 7)
286. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
287. Western blot trouble shooting - (reply: 3)
288. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
289. two bands on WB-alternative use of start codons? - (reply: 2)
290. TCA Precipitation to remove albumin - (reply: 6)
291. adjusting pH of 4x stacking buffer - (reply: 3)
292. No WB bands for H2AX at all...! - (reply: 10)
293. any tips for doing Fluorescence Western Blot? - (reply: 2)
294. Unknown silver/grey residue left after transfer. - (reply: 2)
295. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
296. Changes to housekeeping loading controls after drug treatment - (reply: 2)
297. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
298. Multiple bands on blot with one antibody - (reply: 1)
299. sedimentation of cytosolic proteins - (reply: 2)
300. Determining pI of a protein - (reply: 3)

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