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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
902. Ladder but no protein - (reply: 4)
903. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
904. my membranes are blue! - silly mistake, what to do? (reply: 1)
905. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
906. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
907. Primary Antibody for SPDEF - (reply: 1)
908. Detecting Patterns of phosphorylated protein - (reply: 1)
909. Sample Diffusion Before Applying Current - (reply: 4)
910. IP and western blot - (reply: 5)
911. Semi-dry transfer conditions - Volts or Amps (reply: 1)
912. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
913. Destain after PoneauS - (reply: 6)
914. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
915. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
916. Variability in GAPDH - (reply: 5)
917. nitrocellulose or PVDF - (reply: 7)
918. crappy bands after developing - (reply: 3)
919. cloudy gel - (reply: 2)
920. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
921. Beginner Question - (reply: 6)
922. What's the problem with this SDS-PAGE? - (reply: 8)
923. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
924. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
925. Stripping question - (reply: 3)
926. Smears after transfer - (reply: 2)
927. Accidentally Froze Transfer Buffer - (reply: 1)
928. ImageJ analysis on IFs - (reply: 11)
929. Equal loading for Supernatant western blots??? - (reply: 1)
930. Ponceau Staining Question - (reply: 1)

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