Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
902. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
903. SDS-Page issue - (reply: 5)
904. Fluorescent visualization of Western blot - (reply: 2)
905. MEthanol in NC membrane - (reply: 7)
906. Pre stained markers - (reply: 4)
907. Laminin 5 antibody - (reply: 1)
908. Molecular weight marker - got different pattern! (reply: 13)
909. SDS ladder - which type works better? (reply: 3)
910. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
911. the sensitive amount of protein for western blotting - (reply: 3)
912. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
913. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
914. How to determine final amount of 20 ng protein per WB well - (reply: 8)
915. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
916. nonreducing antibody in reducing condition - (reply: 1)
917. High Molecular weight protein separation - (reply: 2)
918. Secreted protein for loading control - (reply: 2)
919. Only borders stained in Silver!!! - (reply: 7)
920. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
921. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
922. bromophenol blue - (reply: 10)
923. Native Page transfer/detection problems - (reply: 1)
924. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
925. nuclear loading control - (reply: 2)
926. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
927. Large protein transfer problem - (reply: 8)
928. overloading GAPDH but nice band of interest - (reply: 2)
929. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
930. Could I denature the proteins that were transferred on a membrane? - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37