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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. NFkB western blotting - (reply: 3)
902. Mannitol in Lysis Buffer - (reply: 1)
903. how long will a 1M DTT solution will last in -20C - (reply: 5)
904. pellet became insoluble after boiling. - (reply: 4)
905. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
906. Ladder but no protein - (reply: 4)
907. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
908. my membranes are blue! - silly mistake, what to do? (reply: 1)
909. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
910. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
911. Primary Antibody for SPDEF - (reply: 1)
912. Detecting Patterns of phosphorylated protein - (reply: 1)
913. Sample Diffusion Before Applying Current - (reply: 4)
914. IP and western blot - (reply: 5)
915. Semi-dry transfer conditions - Volts or Amps (reply: 1)
916. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
917. Destain after PoneauS - (reply: 6)
918. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
919. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
920. Variability in GAPDH - (reply: 5)
921. nitrocellulose or PVDF - (reply: 7)
922. crappy bands after developing - (reply: 3)
923. cloudy gel - (reply: 2)
924. why BOIL.. samples for sdspage - sample + sample buffer + 'BOIL' 2 mins (reply: 9)
925. Beginner Question - (reply: 6)
926. What's the problem with this SDS-PAGE? - (reply: 8)
927. Crazy: ab burnout or something? Higher quant had lower signal? - (reply: 1)
928. Antibody Validation - Please help me! THis is very Urgent (reply: 2)
929. Stripping question - (reply: 3)
930. Smears after transfer - (reply: 2)

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