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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. small protein blotting - (reply: 8)
902. how stable is alpha mouse HRP? - left on the bench (reply: 2)
903. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
904. Too many bands in my SDS-PAGE - (reply: 5)
905. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
906. Uneven bands in SDS-PAGE - (reply: 5)
907. Can primary antibody be reused ? - (reply: 6)
908. Preparation of tree tissue for western blot - (reply: 1)
909. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
910. direct lysis in Laemmli buffer - (reply: 4)
911. anti-FLAG (M2) cross reaction - (reply: 6)
912. NFkB western blotting - (reply: 3)
913. Mannitol in Lysis Buffer - (reply: 1)
914. how long will a 1M DTT solution will last in -20C - (reply: 5)
915. pellet became insoluble after boiling. - (reply: 4)
916. Pre-Making your own polyacrylamide gels - Short term storage options (reply: 4)
917. Ladder but no protein - (reply: 4)
918. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
919. my membranes are blue! - silly mistake, what to do? (reply: 1)
920. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
921. Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
922. Primary Antibody for SPDEF - (reply: 1)
923. Detecting Patterns of phosphorylated protein - (reply: 1)
924. Sample Diffusion Before Applying Current - (reply: 4)
925. IP and western blot - (reply: 5)
926. Semi-dry transfer conditions - Volts or Amps (reply: 1)
927. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
928. Destain after PoneauS - (reply: 6)
929. ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
930. Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)

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