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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. SDS-PAGE irregular and difuse bands - what's your opinion? (reply: 8)
902. SDS-PAGE Cell lysis problems - (reply: 3)
903. Using Photoshop etc on Western bot images - (reply: 8)
904. Ghost bands in western blot - Western blotting problems (reply: 9)
905. Why immunoblotting of actin coming at higher Molec. Weight - (reply: 1)
906. x-ray films vs direct imaging - Preferred method for western development (reply: 17)
907. proteins stuck at interface between stack and separating gel - (reply: 2)
908. Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins (reply: 16)
909. Lost my proteins ! - (reply: 3)
910. beginner's Questions on Western Blotting - (reply: 4)
911. lost signal - (reply: 4)
912. Using NaN3 to quench HRP conjugated secondary Ab - (reply: 1)
913. White speckles on western blots - Why?? :( (reply: 3)
914. Blocking buffer as antibody dilutent? - (reply: 4)
915. How to prepare colloidal coomassie blue? - ingredients can not dissolve (reply: 4)
916. problem with SDS-PAGE - Sample stuck in the wells of the gel (reply: 5)
917. protein quantification by bradford - (reply: 3)
918. protein extraction from tumor samples containing matrigel? - (reply: 2)
919. western blot - western blot (reply: 17)
920. Amount of protein loaded per well? - (reply: 4)
921. Casp3 - (reply: 2)
922. Anti-FLAG antibody help - Troubleshooting for Sigma anti-FLAG (A8592) (reply: 3)
923. taking protein out of membrane - (reply: 1)
924. prearing gel in advance - (reply: 6)
925. freezing after reducing and boiling - (reply: 10)
926. Invitrogen iBlot - (reply: 6)
927. Even volumes of different samples - (reply: 3)
928. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
929. A single band on WB smaller than expected - (reply: 3)
930. Ponceau staining after primary and secondary? - (reply: 5)

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