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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
902. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
903. bromophenol blue - (reply: 10)
904. Native Page transfer/detection problems - (reply: 1)
905. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
906. nuclear loading control - (reply: 2)
907. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
908. Large protein transfer problem - (reply: 8)
909. overloading GAPDH but nice band of interest - (reply: 2)
910. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
911. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
912. Quantiying bands. - Determination of protein contens (reply: 2)
913. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
914. Strange lane pattern, yes/no pattern - (reply: 3)
915. Native PAGE w. Western protein marker - (reply: 4)
916. protein stuck - (reply: 3)
917. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
918. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
919. changing colour of front dye in sds-page - (reply: 10)
920. small protein blotting - (reply: 8)
921. how stable is alpha mouse HRP? - left on the bench (reply: 2)
922. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
923. Too many bands in my SDS-PAGE - (reply: 5)
924. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
925. Uneven bands in SDS-PAGE - (reply: 5)
926. Can primary antibody be reused ? - (reply: 6)
927. Preparation of tree tissue for western blot - (reply: 1)
928. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
929. direct lysis in Laemmli buffer - (reply: 4)
930. anti-FLAG (M2) cross reaction - (reply: 6)

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