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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
902. nonreducing antibody in reducing condition - (reply: 1)
903. High Molecular weight protein separation - (reply: 2)
904. Secreted protein for loading control - (reply: 2)
905. Only borders stained in Silver!!! - (reply: 7)
906. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
907. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
908. bromophenol blue - (reply: 10)
909. Native Page transfer/detection problems - (reply: 1)
910. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
911. nuclear loading control - (reply: 2)
912. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
913. Large protein transfer problem - (reply: 8)
914. overloading GAPDH but nice band of interest - (reply: 2)
915. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
916. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
917. Quantiying bands. - Determination of protein contens (reply: 2)
918. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
919. Strange lane pattern, yes/no pattern - (reply: 3)
920. Native PAGE w. Western protein marker - (reply: 4)
921. protein stuck - (reply: 3)
922. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
923. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
924. changing colour of front dye in sds-page - (reply: 10)
925. small protein blotting - (reply: 8)
926. how stable is alpha mouse HRP? - left on the bench (reply: 2)
927. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
928. Too many bands in my SDS-PAGE - (reply: 5)
929. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
930. Uneven bands in SDS-PAGE - (reply: 5)

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