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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
902. How to determine final amount of 20 ng protein per WB well - (reply: 8)
903. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
904. nonreducing antibody in reducing condition - (reply: 1)
905. High Molecular weight protein separation - (reply: 2)
906. Secreted protein for loading control - (reply: 2)
907. Only borders stained in Silver!!! - (reply: 7)
908. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
909. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
910. bromophenol blue - (reply: 10)
911. Native Page transfer/detection problems - (reply: 1)
912. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
913. nuclear loading control - (reply: 2)
914. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
915. Large protein transfer problem - (reply: 8)
916. overloading GAPDH but nice band of interest - (reply: 2)
917. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
918. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
919. Quantiying bands. - Determination of protein contens (reply: 2)
920. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
921. Strange lane pattern, yes/no pattern - (reply: 3)
922. Native PAGE w. Western protein marker - (reply: 4)
923. protein stuck - (reply: 3)
924. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
925. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
926. changing colour of front dye in sds-page - (reply: 10)
927. small protein blotting - (reply: 8)
928. how stable is alpha mouse HRP? - left on the bench (reply: 2)
929. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
930. Too many bands in my SDS-PAGE - (reply: 5)

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