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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
902. SDS-Page issue - (reply: 5)
903. Fluorescent visualization of Western blot - (reply: 2)
904. MEthanol in NC membrane - (reply: 7)
905. Pre stained markers - (reply: 4)
906. Laminin 5 antibody - (reply: 1)
907. Molecular weight marker - got different pattern! (reply: 13)
908. SDS ladder - which type works better? (reply: 3)
909. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
910. the sensitive amount of protein for western blotting - (reply: 3)
911. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
912. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
913. How to determine final amount of 20 ng protein per WB well - (reply: 8)
914. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
915. nonreducing antibody in reducing condition - (reply: 1)
916. High Molecular weight protein separation - (reply: 2)
917. Secreted protein for loading control - (reply: 2)
918. Only borders stained in Silver!!! - (reply: 7)
919. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
920. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
921. bromophenol blue - (reply: 10)
922. Native Page transfer/detection problems - (reply: 1)
923. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
924. nuclear loading control - (reply: 2)
925. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
926. Large protein transfer problem - (reply: 8)
927. overloading GAPDH but nice band of interest - (reply: 2)
928. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
929. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
930. Quantiying bands. - Determination of protein contens (reply: 2)

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