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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. Non Reducing SDS protein ladder?? - (reply: 4)
902. Protein Free Blocking Buffers - (reply: 1)
903. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
904. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
905. SDS-Page issue - (reply: 5)
906. Fluorescent visualization of Western blot - (reply: 2)
907. MEthanol in NC membrane - (reply: 7)
908. Pre stained markers - (reply: 4)
909. Laminin 5 antibody - (reply: 1)
910. Molecular weight marker - got different pattern! (reply: 13)
911. SDS ladder - which type works better? (reply: 3)
912. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
913. the sensitive amount of protein for western blotting - (reply: 3)
914. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
915. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
916. How to determine final amount of 20 ng protein per WB well - (reply: 8)
917. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
918. nonreducing antibody in reducing condition - (reply: 1)
919. High Molecular weight protein separation - (reply: 2)
920. Secreted protein for loading control - (reply: 2)
921. Only borders stained in Silver!!! - (reply: 7)
922. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
923. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
924. bromophenol blue - (reply: 10)
925. Native Page transfer/detection problems - (reply: 1)
926. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
927. nuclear loading control - (reply: 2)
928. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
929. Large protein transfer problem - (reply: 8)
930. overloading GAPDH but nice band of interest - (reply: 2)

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