Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. Protein precipitation by TCA acid + acetone - (reply: 4)
902. Non Reducing SDS protein ladder?? - (reply: 4)
903. Protein Free Blocking Buffers - (reply: 1)
904. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
905. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
906. SDS-Page issue - (reply: 5)
907. Fluorescent visualization of Western blot - (reply: 2)
908. MEthanol in NC membrane - (reply: 7)
909. Pre stained markers - (reply: 4)
910. Laminin 5 antibody - (reply: 1)
911. Molecular weight marker - got different pattern! (reply: 13)
912. SDS ladder - which type works better? (reply: 3)
913. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
914. the sensitive amount of protein for western blotting - (reply: 3)
915. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
916. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
917. How to determine final amount of 20 ng protein per WB well - (reply: 8)
918. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
919. nonreducing antibody in reducing condition - (reply: 1)
920. High Molecular weight protein separation - (reply: 2)
921. Secreted protein for loading control - (reply: 2)
922. Only borders stained in Silver!!! - (reply: 7)
923. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
924. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
925. bromophenol blue - (reply: 10)
926. Native Page transfer/detection problems - (reply: 1)
927. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
928. nuclear loading control - (reply: 2)
929. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
930. Large protein transfer problem - (reply: 8)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37