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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. SDS ladder - which type works better? (reply: 3)
902. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
903. the sensitive amount of protein for western blotting - (reply: 3)
904. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
905. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
906. How to determine final amount of 20 ng protein per WB well - (reply: 8)
907. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
908. nonreducing antibody in reducing condition - (reply: 1)
909. High Molecular weight protein separation - (reply: 2)
910. Secreted protein for loading control - (reply: 2)
911. Only borders stained in Silver!!! - (reply: 7)
912. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
913. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
914. bromophenol blue - (reply: 10)
915. Native Page transfer/detection problems - (reply: 1)
916. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
917. nuclear loading control - (reply: 2)
918. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
919. Large protein transfer problem - (reply: 8)
920. overloading GAPDH but nice band of interest - (reply: 2)
921. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
922. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
923. Quantiying bands. - Determination of protein contens (reply: 2)
924. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
925. Strange lane pattern, yes/no pattern - (reply: 3)
926. Native PAGE w. Western protein marker - (reply: 4)
927. protein stuck - (reply: 3)
928. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
929. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
930. changing colour of front dye in sds-page - (reply: 10)

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