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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
901. Bands visible in membrane but not in the film - ?? (reply: 10)
902. non desired brigther bands - (reply: 7)
903. Dimer or monomer - (reply: 11)
904. Protein precipitation by TCA acid + acetone - (reply: 4)
905. Non Reducing SDS protein ladder?? - (reply: 4)
906. Protein Free Blocking Buffers - (reply: 1)
907. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
908. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
909. SDS-Page issue - (reply: 5)
910. Fluorescent visualization of Western blot - (reply: 2)
911. MEthanol in NC membrane - (reply: 7)
912. Pre stained markers - (reply: 4)
913. Laminin 5 antibody - (reply: 1)
914. Molecular weight marker - got different pattern! (reply: 13)
915. SDS ladder - which type works better? (reply: 3)
916. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
917. the sensitive amount of protein for western blotting - (reply: 3)
918. Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity (reply: 3)
919. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
920. How to determine final amount of 20 ng protein per WB well - (reply: 8)
921. Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n (reply: 4)
922. nonreducing antibody in reducing condition - (reply: 1)
923. High Molecular weight protein separation - (reply: 2)
924. Secreted protein for loading control - (reply: 2)
925. Only borders stained in Silver!!! - (reply: 7)
926. Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
927. How Best to Clean the Sonicator Tip Between Samples? - (reply: 5)
928. bromophenol blue - (reply: 10)
929. Native Page transfer/detection problems - (reply: 1)
930. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)

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