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301. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
302. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
303. How to avoid gel breakage after electrophoresis - (reply: 5)
304. What is an Input in SDS-PAGE? - (reply: 1)
305. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
306. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
307. Good loading control for native gels? - (reply: 1)
308. Please help with odd bands and smears on Western blot - (reply: 4)
309. How to load equal amount of protein without using spectramax - (reply: 4)
310. Western blot membrane imaging - (reply: 7)
311. Detection of T7 RNA polymerase by WB - (reply: 6)
312. Shift in the position of band onto the western blot - (reply: 3)
313. Ghost and inverse bands and high background. Help! - (reply: 3)
314. No resolving of protein/bands below 50kDa :-s - (reply: 5)
315. Problems with wet transfer blot - (reply: 2)
316. 5% BSA blocking buffer - (reply: 3)
317. Storage of Membrane before addition of primary antibody - (reply: 3)
318. "Mickey Mouse" bands - (reply: 5)
319. SDS-PAGE : when the transfer stops - (reply: 1)
320. confusing Western results - (reply: 5)
321. aditional band on WB - (reply: 1)
322. Transferring >150 kDA proteins conditions - (reply: 3)
323. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
324. TAU gel electrophoresis of histones - (reply: 4)
325. Bands dark on sides and light in middle - (reply: 7)
326. Addition of sample loading buffer, before or after heating - (reply: 5)
327. question about protein expression normalization. - (reply: 6)
328. Protein bands not migrating - (reply: 3)
329. Help with high background (have tried most trouble shooting) - (reply: 8)
330. Tumor protein precipitation at denaturing step? - (reply: 1)
302. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
303. How to avoid gel breakage after electrophoresis - (reply: 5)
304. What is an Input in SDS-PAGE? - (reply: 1)
305. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
306. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
307. Good loading control for native gels? - (reply: 1)
308. Please help with odd bands and smears on Western blot - (reply: 4)
309. How to load equal amount of protein without using spectramax - (reply: 4)
310. Western blot membrane imaging - (reply: 7)
311. Detection of T7 RNA polymerase by WB - (reply: 6)
312. Shift in the position of band onto the western blot - (reply: 3)
313. Ghost and inverse bands and high background. Help! - (reply: 3)
314. No resolving of protein/bands below 50kDa :-s - (reply: 5)
315. Problems with wet transfer blot - (reply: 2)
316. 5% BSA blocking buffer - (reply: 3)
317. Storage of Membrane before addition of primary antibody - (reply: 3)
318. "Mickey Mouse" bands - (reply: 5)
319. SDS-PAGE : when the transfer stops - (reply: 1)
320. confusing Western results - (reply: 5)
321. aditional band on WB - (reply: 1)
322. Transferring >150 kDA proteins conditions - (reply: 3)
323. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
324. TAU gel electrophoresis of histones - (reply: 4)
325. Bands dark on sides and light in middle - (reply: 7)
326. Addition of sample loading buffer, before or after heating - (reply: 5)
327. question about protein expression normalization. - (reply: 6)
328. Protein bands not migrating - (reply: 3)
329. Help with high background (have tried most trouble shooting) - (reply: 8)
330. Tumor protein precipitation at denaturing step? - (reply: 1)