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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. Phospho-ATF-2 coming up different sizes - (reply: 1)
302. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
303. A possible protocol for western blotting troubleshooting? - (reply: 1)
304. Vertical Vs horizontal electrophoresis - (reply: 3)
305. Reprobing with the same antibody - (reply: 3)
306. SDS-PAGE problem with staining yellow background - (reply: 3)
307. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
308. Western blot narrow bands - (reply: 1)
309. Grainy image and no signal on Western using ChemiDoc - (reply: 6)
310. Western Blot Antibody Epitope - (reply: 1)
311. Western blot band anaylisis - (reply: 3)
312. protein aggregation (?) in gelatin zymography - (reply: 1)
313. Use of wrong TBS concentration - (reply: 1)
314. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
315. SDS-PAGE staining for digestion assay - (reply: 1)
316. Can I improve my primary antibody? - (reply: 2)
317. got band in control positive but not in sample... - (reply: 2)
318. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
319. Problems with IPs - (reply: 2)
320. Band is at the wrong size, but same for all antibodies - (reply: 6)
321. Problems with western blot detection - (reply: 1)
322. Methods of concentrating total protein extracts - (reply: 2)
323. Using serum as the sample in Western blot - (reply: 3)
324. Puzzeling western blot results, missing band - (reply: 3)
325. Secondary AB blocking - (reply: 1)
326. western blood immunodetection Ab - (reply: 1)
327. Excess bromophenol blue in 2-D rehydration buffer - (reply: 1)
328. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
329. What does percentage mean when dealing with stacking and running gel for SDS pag - (reply: 6)
330. What percentage should I use for a stacking gel? - (reply: 1)

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