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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. Addition of sample loading buffer, before or after heating - (reply: 5)
302. question about protein expression normalization. - (reply: 6)
303. Protein bands not migrating - (reply: 3)
304. Help with high background (have tried most trouble shooting) - (reply: 8)
305. Tumor protein precipitation at denaturing step? - (reply: 1)
306. How to prepare blood serum for western blot? - (reply: 3)
307. TNF alpha and IL 1b Western Blot - (reply: 1)
308. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
309. SDS-PAGE trouble with protein concentration - (reply: 2)
310. Acrylamid - (reply: 5)
311. Detecting doublet bands in Western for eGFP control - (reply: 1)
312. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
313. Protein Size 50 kDa higher than expected - (reply: 3)
314. Acid urea gel electrophoresis (AUGE) - (reply: 7)
315. Phospho-protein vs total protein Western blot - (reply: 5)
316. storing the loaded gel before running it - (reply: 7)
317. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
318. Western blot trouble shooting - (reply: 3)
319. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
320. two bands on WB-alternative use of start codons? - (reply: 2)
321. TCA Precipitation to remove albumin - (reply: 6)
322. adjusting pH of 4x stacking buffer - (reply: 3)
323. No WB bands for H2AX at all...! - (reply: 10)
324. any tips for doing Fluorescence Western Blot? - (reply: 2)
325. Unknown silver/grey residue left after transfer. - (reply: 2)
326. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
327. Changes to housekeeping loading controls after drug treatment - (reply: 2)
328. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
329. Multiple bands on blot with one antibody - (reply: 1)
330. sedimentation of cytosolic proteins - (reply: 2)

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