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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
302. Reuse of SDS-PAGE electrode buffers - (reply: 2)
303. Upside-down smiley occurs when running SDS PAGE - (reply: 3)
304. Western transfer tank heating up - (reply: 2)
305. Tricine PAG - (reply: 1)
306. Simple Western = a western blot revolution? - (reply: 2)
307. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
308. Best phospho-ERK antibody - (reply: 3)
309. what are the best transfer conditions? - (reply: 4)
310. Cell Envelope Proteins Smeared - (reply: 1)
311. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
312. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
313. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
314. restaining of coommassie destained gel - (reply: 1)
315. Secondary antibody overnight? - (reply: 5)
316. Problems with protein estimation and western blots - (reply: 1)
317. Loading controls - (reply: 1)
318. Quantity of protein to charge - (reply: 1)
319. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
320. Tris-Hcl becomes cloudy. - (reply: 3)
321. Can FLAG IP, but cannot detect with Western - (reply: 4)
322. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
323. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
324. Native PAGE, interaction of proteins, validity - (reply: 4)
325. Smeared transfer on nitrocellulose membrane - (reply: 2)
326. blocking and primary antibody in different buffers? - (reply: 1)
327. Same non specific band for different target proteins - (reply: 1)
328. Types of 96-well plates used for BCA protein quantification - (reply: 1)
329. Dot blot halo - (reply: 4)
330. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)

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