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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. confusing Western results - (reply: 5)
302. aditional band on WB - (reply: 1)
303. Transferring >150 kDA proteins conditions - (reply: 3)
304. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
305. TAU gel electrophoresis of histones - (reply: 4)
306. Bands dark on sides and light in middle - (reply: 7)
307. Addition of sample loading buffer, before or after heating - (reply: 5)
308. question about protein expression normalization. - (reply: 6)
309. Protein bands not migrating - (reply: 3)
310. Help with high background (have tried most trouble shooting) - (reply: 8)
311. Tumor protein precipitation at denaturing step? - (reply: 1)
312. How to prepare blood serum for western blot? - (reply: 3)
313. TNF alpha and IL 1b Western Blot - (reply: 1)
314. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
315. SDS-PAGE trouble with protein concentration - (reply: 2)
316. Acrylamid - (reply: 5)
317. Detecting doublet bands in Western for eGFP control - (reply: 1)
318. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
319. Protein Size 50 kDa higher than expected - (reply: 3)
320. Acid urea gel electrophoresis (AUGE) - (reply: 7)
321. Phospho-protein vs total protein Western blot - (reply: 5)
322. storing the loaded gel before running it - (reply: 7)
323. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
324. Western blot trouble shooting - (reply: 3)
325. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
326. two bands on WB-alternative use of start codons? - (reply: 2)
327. TCA Precipitation to remove albumin - (reply: 6)
328. adjusting pH of 4x stacking buffer - (reply: 3)
329. No WB bands for H2AX at all...! - (reply: 10)
330. any tips for doing Fluorescence Western Blot? - (reply: 2)

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