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301. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
302. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
303. Native PAGE, interaction of proteins, validity - (reply: 4)
304. Smeared transfer on nitrocellulose membrane - (reply: 2)
305. blocking and primary antibody in different buffers? - (reply: 1)
306. Same non specific band for different target proteins - (reply: 1)
307. Types of 96-well plates used for BCA protein quantification - (reply: 1)
308. Dot blot halo - (reply: 4)
309. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
310. Western blot and isoelectric point - (reply: 1)
311. Western Blot Developing Cassette - (reply: 1)
312. Pull down assay and Co- IP experiment - (reply: 6)
313. Trouble detecting Histaq - (reply: 4)
314. Western gels running super slow! - (reply: 3)
315. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
316. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
317. WB parametric or non-parametric - (reply: 6)
318. Trouble with the GFP signal - (reply: 1)
319. Intern Needing Help - (reply: 3)
320. SDS-Electrophoresis, Dye color change - (reply: 1)
321. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
322. Trouble with equal loading - (reply: 8)
323. how many times striping can b done of the blot - (reply: 9)
324. SDS-PAGE of negatively charged protein - (reply: 4)
325. Protection Lanes? - (reply: 4)
326. Tie-2 westerns high background black lanes - (reply: 13)
327. BSA concentration for blocking - (reply: 5)
328. Identically loaded western bands loose intensity from left to right - (reply: 3)
329. eIF4G western blot - (reply: 3)
330. smear bands in western blot - (reply: 12)
302. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
303. Native PAGE, interaction of proteins, validity - (reply: 4)
304. Smeared transfer on nitrocellulose membrane - (reply: 2)
305. blocking and primary antibody in different buffers? - (reply: 1)
306. Same non specific band for different target proteins - (reply: 1)
307. Types of 96-well plates used for BCA protein quantification - (reply: 1)
308. Dot blot halo - (reply: 4)
309. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
310. Western blot and isoelectric point - (reply: 1)
311. Western Blot Developing Cassette - (reply: 1)
312. Pull down assay and Co- IP experiment - (reply: 6)
313. Trouble detecting Histaq - (reply: 4)
314. Western gels running super slow! - (reply: 3)
315. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
316. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
317. WB parametric or non-parametric - (reply: 6)
318. Trouble with the GFP signal - (reply: 1)
319. Intern Needing Help - (reply: 3)
320. SDS-Electrophoresis, Dye color change - (reply: 1)
321. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
322. Trouble with equal loading - (reply: 8)
323. how many times striping can b done of the blot - (reply: 9)
324. SDS-PAGE of negatively charged protein - (reply: 4)
325. Protection Lanes? - (reply: 4)
326. Tie-2 westerns high background black lanes - (reply: 13)
327. BSA concentration for blocking - (reply: 5)
328. Identically loaded western bands loose intensity from left to right - (reply: 3)
329. eIF4G western blot - (reply: 3)
330. smear bands in western blot - (reply: 12)