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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. Stripping the membrane for LiCor - (reply: 1)
302. 17 kDa ideal transfer voltage - PVDF - (reply: 4)
303. Lysis buffer - (reply: 2)
304. SDS Page and WB: Wrong size bands - (reply: 2)
305. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
306. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
307. How to avoid gel breakage after electrophoresis - (reply: 5)
308. What is an Input in SDS-PAGE? - (reply: 1)
309. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
310. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
311. Good loading control for native gels? - (reply: 1)
312. Please help with odd bands and smears on Western blot - (reply: 4)
313. How to load equal amount of protein without using spectramax - (reply: 4)
314. Western blot membrane imaging - (reply: 7)
315. Detection of T7 RNA polymerase by WB - (reply: 6)
316. Shift in the position of band onto the western blot - (reply: 3)
317. Ghost and inverse bands and high background. Help! - (reply: 3)
318. No resolving of protein/bands below 50kDa :-s - (reply: 5)
319. Problems with wet transfer blot - (reply: 2)
320. 5% BSA blocking buffer - (reply: 3)
321. Storage of Membrane before addition of primary antibody - (reply: 3)
322. "Mickey Mouse" bands - (reply: 5)
323. SDS-PAGE : when the transfer stops - (reply: 1)
324. confusing Western results - (reply: 5)
325. aditional band on WB - (reply: 1)
326. Transferring >150 kDA proteins conditions - (reply: 3)
327. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
328. TAU gel electrophoresis of histones - (reply: 4)
329. Bands dark on sides and light in middle - (reply: 7)
330. Addition of sample loading buffer, before or after heating - (reply: 5)

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