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301. What is an Input in SDS-PAGE? - (reply: 1)
302. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
303. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
304. Good loading control for native gels? - (reply: 1)
305. Please help with odd bands and smears on Western blot - (reply: 4)
306. How to load equal amount of protein without using spectramax - (reply: 4)
307. Western blot membrane imaging - (reply: 7)
308. Detection of T7 RNA polymerase by WB - (reply: 6)
309. Shift in the position of band onto the western blot - (reply: 3)
310. Ghost and inverse bands and high background. Help! - (reply: 3)
311. No resolving of protein/bands below 50kDa :-s - (reply: 5)
312. Problems with wet transfer blot - (reply: 2)
313. 5% BSA blocking buffer - (reply: 3)
314. Storage of Membrane before addition of primary antibody - (reply: 3)
315. "Mickey Mouse" bands - (reply: 5)
316. SDS-PAGE : when the transfer stops - (reply: 1)
317. confusing Western results - (reply: 5)
318. aditional band on WB - (reply: 1)
319. Transferring >150 kDA proteins conditions - (reply: 3)
320. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
321. TAU gel electrophoresis of histones - (reply: 4)
322. Bands dark on sides and light in middle - (reply: 7)
323. Addition of sample loading buffer, before or after heating - (reply: 5)
324. question about protein expression normalization. - (reply: 6)
325. Protein bands not migrating - (reply: 3)
326. Help with high background (have tried most trouble shooting) - (reply: 8)
327. Tumor protein precipitation at denaturing step? - (reply: 1)
328. How to prepare blood serum for western blot? - (reply: 3)
329. TNF alpha and IL 1b Western Blot - (reply: 1)
330. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
302. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
303. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
304. Good loading control for native gels? - (reply: 1)
305. Please help with odd bands and smears on Western blot - (reply: 4)
306. How to load equal amount of protein without using spectramax - (reply: 4)
307. Western blot membrane imaging - (reply: 7)
308. Detection of T7 RNA polymerase by WB - (reply: 6)
309. Shift in the position of band onto the western blot - (reply: 3)
310. Ghost and inverse bands and high background. Help! - (reply: 3)
311. No resolving of protein/bands below 50kDa :-s - (reply: 5)
312. Problems with wet transfer blot - (reply: 2)
313. 5% BSA blocking buffer - (reply: 3)
314. Storage of Membrane before addition of primary antibody - (reply: 3)
315. "Mickey Mouse" bands - (reply: 5)
316. SDS-PAGE : when the transfer stops - (reply: 1)
317. confusing Western results - (reply: 5)
318. aditional band on WB - (reply: 1)
319. Transferring >150 kDA proteins conditions - (reply: 3)
320. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
321. TAU gel electrophoresis of histones - (reply: 4)
322. Bands dark on sides and light in middle - (reply: 7)
323. Addition of sample loading buffer, before or after heating - (reply: 5)
324. question about protein expression normalization. - (reply: 6)
325. Protein bands not migrating - (reply: 3)
326. Help with high background (have tried most trouble shooting) - (reply: 8)
327. Tumor protein precipitation at denaturing step? - (reply: 1)
328. How to prepare blood serum for western blot? - (reply: 3)
329. TNF alpha and IL 1b Western Blot - (reply: 1)
330. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)