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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. storing the loaded gel before running it - (reply: 7)
302. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
303. Western blot trouble shooting - (reply: 3)
304. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
305. two bands on WB-alternative use of start codons? - (reply: 2)
306. TCA Precipitation to remove albumin - (reply: 6)
307. adjusting pH of 4x stacking buffer - (reply: 3)
308. No WB bands for H2AX at all...! - (reply: 10)
309. any tips for doing Fluorescence Western Blot? - (reply: 2)
310. Unknown silver/grey residue left after transfer. - (reply: 2)
311. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
312. Changes to housekeeping loading controls after drug treatment - (reply: 2)
313. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
314. Multiple bands on blot with one antibody - (reply: 1)
315. sedimentation of cytosolic proteins - (reply: 2)
316. Determining pI of a protein - (reply: 3)
317. Non-Reduced Western Blot - (reply: 5)
318. Incomplete transfer - (reply: 5)
319. can coommassie stained gel be western blot - (reply: 5)
320. Wet transfer: western blot - (reply: 3)
321. Reprobing a PVDF membrane which has gone dry - (reply: 1)
322. Non-specific bands and fuzzy bands - (reply: 1)
323. protein showing up around 50 kda rather than 20 kda - (reply: 2)
324. Help for western blot wet transfer blurry bands - (reply: 2)
325. anti-BRCA1 antibody specificity issues - (reply: 2)
326. Concentrating a diluted protein and protein determination - (reply: 4)
327. Blurry bands ... - (reply: 12)
328. Dot blotting problems - (reply: 3)
329. 2 primary antibodies, both binding actin...they shouldn't be :( - (reply: 3)
330. Uneven lane widths during western blots - (reply: 10)

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