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301. 17 kDa ideal transfer voltage - PVDF - (reply: 4)
302. Lysis buffer - (reply: 2)
303. SDS Page and WB: Wrong size bands - (reply: 2)
304. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
305. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
306. How to avoid gel breakage after electrophoresis - (reply: 5)
307. What is an Input in SDS-PAGE? - (reply: 1)
308. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
309. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
310. Good loading control for native gels? - (reply: 1)
311. Please help with odd bands and smears on Western blot - (reply: 4)
312. How to load equal amount of protein without using spectramax - (reply: 4)
313. Western blot membrane imaging - (reply: 7)
314. Detection of T7 RNA polymerase by WB - (reply: 6)
315. Shift in the position of band onto the western blot - (reply: 3)
316. Ghost and inverse bands and high background. Help! - (reply: 3)
317. No resolving of protein/bands below 50kDa :-s - (reply: 5)
318. Problems with wet transfer blot - (reply: 2)
319. 5% BSA blocking buffer - (reply: 3)
320. Storage of Membrane before addition of primary antibody - (reply: 3)
321. "Mickey Mouse" bands - (reply: 5)
322. SDS-PAGE : when the transfer stops - (reply: 1)
323. confusing Western results - (reply: 5)
324. aditional band on WB - (reply: 1)
325. Transferring >150 kDA proteins conditions - (reply: 3)
326. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
327. TAU gel electrophoresis of histones - (reply: 4)
328. Bands dark on sides and light in middle - (reply: 7)
329. Addition of sample loading buffer, before or after heating - (reply: 5)
330. question about protein expression normalization. - (reply: 6)
302. Lysis buffer - (reply: 2)
303. SDS Page and WB: Wrong size bands - (reply: 2)
304. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
305. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
306. How to avoid gel breakage after electrophoresis - (reply: 5)
307. What is an Input in SDS-PAGE? - (reply: 1)
308. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
309. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
310. Good loading control for native gels? - (reply: 1)
311. Please help with odd bands and smears on Western blot - (reply: 4)
312. How to load equal amount of protein without using spectramax - (reply: 4)
313. Western blot membrane imaging - (reply: 7)
314. Detection of T7 RNA polymerase by WB - (reply: 6)
315. Shift in the position of band onto the western blot - (reply: 3)
316. Ghost and inverse bands and high background. Help! - (reply: 3)
317. No resolving of protein/bands below 50kDa :-s - (reply: 5)
318. Problems with wet transfer blot - (reply: 2)
319. 5% BSA blocking buffer - (reply: 3)
320. Storage of Membrane before addition of primary antibody - (reply: 3)
321. "Mickey Mouse" bands - (reply: 5)
322. SDS-PAGE : when the transfer stops - (reply: 1)
323. confusing Western results - (reply: 5)
324. aditional band on WB - (reply: 1)
325. Transferring >150 kDA proteins conditions - (reply: 3)
326. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
327. TAU gel electrophoresis of histones - (reply: 4)
328. Bands dark on sides and light in middle - (reply: 7)
329. Addition of sample loading buffer, before or after heating - (reply: 5)
330. question about protein expression normalization. - (reply: 6)