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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. LC3 Autophagy Huh7 - (reply: 1)
302. lysing mammalian cells in sds - (reply: 3)
303. Western blot mystery - possibly a sample preparation problem? - (reply: 11)
304. How to store a membrane? - (reply: 1)
305. Sample does not sit in the well despite (glycerol checked) - (reply: 4)
306. Western blot sample loading question - (reply: 1)
307. help for the method of meat sample preperation for SDS PAGE 1D - (reply: 4)
308. stripping too much? - (reply: 1)
309. sample precipitation problem in western blotting - (reply: 2)
310. Problem: mouse antibodies with mouse tissues - (reply: 4)
311. which protein can I use as a loading control for nuclear protein fraction? - (reply: 2)
312. Can we use ELISA capturing antibody for Western Blot Primary antibody? - (reply: 1)
313. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
314. Reuse of SDS-PAGE electrode buffers - (reply: 2)
315. Upside-down smiley occurs when running SDS PAGE - (reply: 3)
316. Western transfer tank heating up - (reply: 2)
317. Tricine PAG - (reply: 1)
318. Simple Western = a western blot revolution? - (reply: 2)
319. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
320. Best phospho-ERK antibody - (reply: 3)
321. what are the best transfer conditions? - (reply: 4)
322. Cell Envelope Proteins Smeared - (reply: 1)
323. Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
324. Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
325. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
326. restaining of coommassie destained gel - (reply: 1)
327. Secondary antibody overnight? - (reply: 5)
328. Problems with protein estimation and western blots - (reply: 1)
329. Loading controls - (reply: 1)
330. Quantity of protein to charge - (reply: 1)

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