Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. No resolving of protein/bands below 50kDa :-s - (reply: 5)
302. Problems with wet transfer blot - (reply: 2)
303. 5% BSA blocking buffer - (reply: 3)
304. Storage of Membrane before addition of primary antibody - (reply: 3)
305. "Mickey Mouse" bands - (reply: 5)
306. SDS-PAGE : when the transfer stops - (reply: 1)
307. confusing Western results - (reply: 5)
308. aditional band on WB - (reply: 1)
309. Transferring >150 kDA proteins conditions - (reply: 3)
310. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
311. TAU gel electrophoresis of histones - (reply: 4)
312. Bands dark on sides and light in middle - (reply: 7)
313. Addition of sample loading buffer, before or after heating - (reply: 5)
314. question about protein expression normalization. - (reply: 6)
315. Protein bands not migrating - (reply: 3)
316. Help with high background (have tried most trouble shooting) - (reply: 8)
317. Tumor protein precipitation at denaturing step? - (reply: 1)
318. How to prepare blood serum for western blot? - (reply: 3)
319. TNF alpha and IL 1b Western Blot - (reply: 1)
320. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
321. SDS-PAGE trouble with protein concentration - (reply: 2)
322. Acrylamid - (reply: 5)
323. Detecting doublet bands in Western for eGFP control - (reply: 1)
324. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
325. Protein Size 50 kDa higher than expected - (reply: 3)
326. Acid urea gel electrophoresis (AUGE) - (reply: 7)
327. Phospho-protein vs total protein Western blot - (reply: 5)
328. storing the loaded gel before running it - (reply: 7)
329. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
330. Western blot trouble shooting - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37