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301. developing film manually - (reply: 19)
302. non specific bands - (reply: 7)
303. BSA vs Milk - (reply: 2)
304. Western Blot no target bands, show Non-specific bands - (reply: 5)
305. how critical is the pH of tbst? - (reply: 2)
306. Fc fusion protein smearing on SDS PAGE - (reply: 2)
307. what do you recommend for Semi-Dry unit - (reply: 2)
308. Bands disappear after longer exposure - (reply: 4)
309. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
310. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
311. Antibody Optimization - (reply: 2)
312. Protein agregation in WB samples - (reply: 3)
313. Same Non-specific bands show up for different Abs!! - (reply: 7)
314. what caused bubble band of my WB? - (reply: 4)
315. blotting detection - (reply: 3)
316. Max amount of protein - (reply: 4)
317. Areas of membrane bleached - (reply: 1)
318. asking suggestions for western blot - (reply: 3)
319. Can i apply primary antibody for two nights instead of one? - (reply: 3)
320. Strange bands and large smear in western blot - (reply: 4)
321. Freezing proteins at -80 - (reply: 1)
322. Tris glycine SDS buffer prep pH error - (reply: 2)
323. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
324. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
325. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
326. problems with negative control - (reply: 2)
327. urgent-W.B visualisation on kodak film - (reply: 2)
328. NP 40 lysis buffer - (reply: 1)
329. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
330. Dark western blot film - (reply: 12)
302. non specific bands - (reply: 7)
303. BSA vs Milk - (reply: 2)
304. Western Blot no target bands, show Non-specific bands - (reply: 5)
305. how critical is the pH of tbst? - (reply: 2)
306. Fc fusion protein smearing on SDS PAGE - (reply: 2)
307. what do you recommend for Semi-Dry unit - (reply: 2)
308. Bands disappear after longer exposure - (reply: 4)
309. Housekeeper for Plasma samples in Western Blotting - (reply: 3)
310. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
311. Antibody Optimization - (reply: 2)
312. Protein agregation in WB samples - (reply: 3)
313. Same Non-specific bands show up for different Abs!! - (reply: 7)
314. what caused bubble band of my WB? - (reply: 4)
315. blotting detection - (reply: 3)
316. Max amount of protein - (reply: 4)
317. Areas of membrane bleached - (reply: 1)
318. asking suggestions for western blot - (reply: 3)
319. Can i apply primary antibody for two nights instead of one? - (reply: 3)
320. Strange bands and large smear in western blot - (reply: 4)
321. Freezing proteins at -80 - (reply: 1)
322. Tris glycine SDS buffer prep pH error - (reply: 2)
323. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
324. Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
325. Use of colloidal coomassie stained to western blotting?? - (reply: 5)
326. problems with negative control - (reply: 2)
327. urgent-W.B visualisation on kodak film - (reply: 2)
328. NP 40 lysis buffer - (reply: 1)
329. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
330. Dark western blot film - (reply: 12)