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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. How to load equal amount of protein without using spectramax - (reply: 4)
302. Western blot membrane imaging - (reply: 7)
303. Detection of T7 RNA polymerase by WB - (reply: 6)
304. Shift in the position of band onto the western blot - (reply: 3)
305. Ghost and inverse bands and high background. Help! - (reply: 3)
306. No resolving of protein/bands below 50kDa :-s - (reply: 5)
307. Problems with wet transfer blot - (reply: 2)
308. 5% BSA blocking buffer - (reply: 3)
309. Storage of Membrane before addition of primary antibody - (reply: 3)
310. "Mickey Mouse" bands - (reply: 5)
311. SDS-PAGE : when the transfer stops - (reply: 1)
312. confusing Western results - (reply: 5)
313. aditional band on WB - (reply: 1)
314. Transferring >150 kDA proteins conditions - (reply: 3)
315. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
316. TAU gel electrophoresis of histones - (reply: 4)
317. Bands dark on sides and light in middle - (reply: 7)
318. Addition of sample loading buffer, before or after heating - (reply: 5)
319. question about protein expression normalization. - (reply: 6)
320. Protein bands not migrating - (reply: 3)
321. Help with high background (have tried most trouble shooting) - (reply: 8)
322. Tumor protein precipitation at denaturing step? - (reply: 1)
323. How to prepare blood serum for western blot? - (reply: 3)
324. TNF alpha and IL 1b Western Blot - (reply: 1)
325. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
326. SDS-PAGE trouble with protein concentration - (reply: 2)
327. Acrylamid - (reply: 5)
328. Detecting doublet bands in Western for eGFP control - (reply: 1)
329. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
330. Protein Size 50 kDa higher than expected - (reply: 3)

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