Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. Western blot band anaylisis - (reply: 3)
302. protein aggregation (?) in gelatin zymography - (reply: 1)
303. Use of wrong TBS concentration - (reply: 1)
304. Transfer time optimisation - Overnight low voltage vs Few hours high voltage - (reply: 1)
305. SDS-PAGE staining for digestion assay - (reply: 1)
306. Can I improve my primary antibody? - (reply: 2)
307. got band in control positive but not in sample... - (reply: 2)
308. turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
309. Problems with IPs - (reply: 2)
310. Band is at the wrong size, but same for all antibodies - (reply: 6)
311. Problems with western blot detection - (reply: 1)
312. Methods of concentrating total protein extracts - (reply: 2)
313. Using serum as the sample in Western blot - (reply: 3)
314. Puzzeling western blot results, missing band - (reply: 3)
315. Secondary AB blocking - (reply: 1)
316. western blood immunodetection Ab - (reply: 1)
317. Excess bromophenol blue in 2-D rehydration buffer - (reply: 1)
318. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
319. What does percentage mean when dealing with stacking and running gel for SDS pag - (reply: 6)
320. What percentage should I use for a stacking gel? - (reply: 1)
321. DMSO - (reply: 1)
322. several questions on running SDS-PAGE - (reply: 1)
323. Voltage issues during transfer - (reply: 5)
324. problems with housekeeping antibodies when re-probing membranes - (reply: 3)
325. What percentage gel should I make? - (reply: 1)
326. Western Blot problems-vertical streaking - (reply: 3)
327. Protein extraction from dead cells - (reply: 1)
328. Signal stuck on top of seperating gel - (reply: 1)
329. SDS-PAGE gel and running buffer - (reply: 3)
330. The same strong bands with different primary antibody - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34