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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
302. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
303. Good loading control for native gels? - (reply: 1)
304. Please help with odd bands and smears on Western blot - (reply: 4)
305. How to load equal amount of protein without using spectramax - (reply: 4)
306. Western blot membrane imaging - (reply: 7)
307. Detection of T7 RNA polymerase by WB - (reply: 6)
308. Shift in the position of band onto the western blot - (reply: 3)
309. Ghost and inverse bands and high background. Help! - (reply: 3)
310. No resolving of protein/bands below 50kDa :-s - (reply: 5)
311. Problems with wet transfer blot - (reply: 2)
312. 5% BSA blocking buffer - (reply: 3)
313. Storage of Membrane before addition of primary antibody - (reply: 3)
314. "Mickey Mouse" bands - (reply: 5)
315. SDS-PAGE : when the transfer stops - (reply: 1)
316. confusing Western results - (reply: 5)
317. aditional band on WB - (reply: 1)
318. Transferring >150 kDA proteins conditions - (reply: 3)
319. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
320. TAU gel electrophoresis of histones - (reply: 4)
321. Bands dark on sides and light in middle - (reply: 7)
322. Addition of sample loading buffer, before or after heating - (reply: 5)
323. question about protein expression normalization. - (reply: 6)
324. Protein bands not migrating - (reply: 3)
325. Help with high background (have tried most trouble shooting) - (reply: 8)
326. Tumor protein precipitation at denaturing step? - (reply: 1)
327. How to prepare blood serum for western blot? - (reply: 3)
328. TNF alpha and IL 1b Western Blot - (reply: 1)
329. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
330. SDS-PAGE trouble with protein concentration - (reply: 2)

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