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SDS-PAGE-and-Western-Blotting
301.
the intensity ratio between light chain and heavy chain on SDS-PAGE -
(reply: 3)
302.
How to use miniblotter to perform multiple antibody detection on the same membra -
(reply: 3)
303.
How to avoid gel breakage after electrophoresis -
(reply: 5)
304.
What is an Input in SDS-PAGE? -
(reply: 1)
305.
HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! -
(reply: 2)
306.
Laemmli buffer a bit green or yellow after boiling!!?!? -
(reply: 3)
307.
Good loading control for native gels? -
(reply: 1)
308.
Please help with odd bands and smears on Western blot -
(reply: 4)
309.
How to load equal amount of protein without using spectramax -
(reply: 4)
310.
Western blot membrane imaging -
(reply: 7)
311.
Detection of T7 RNA polymerase by WB -
(reply: 6)
312.
Shift in the position of band onto the western blot -
(reply: 3)
313.
Ghost and inverse bands and high background. Help! -
(reply: 3)
314.
No resolving of protein/bands below 50kDa :-s -
(reply: 5)
315.
Problems with wet transfer blot -
(reply: 2)
316.
5% BSA blocking buffer -
(reply: 3)
317.
Storage of Membrane before addition of primary antibody -
(reply: 3)
318.
"Mickey Mouse" bands -
(reply: 5)
319.
SDS-PAGE : when the transfer stops -
(reply: 1)
320.
confusing Western results -
(reply: 5)
321.
aditional band on WB -
(reply: 1)
322.
Transferring >150 kDA proteins conditions -
(reply: 3)
323.
Does anyone have a reoccuring problem executing Gel electrophoresis? -
(reply: 1)
324.
TAU gel electrophoresis of histones -
(reply: 4)
325.
Bands dark on sides and light in middle -
(reply: 7)
326.
Addition of sample loading buffer, before or after heating -
(reply: 5)
327.
question about protein expression normalization. -
(reply: 6)
328.
Protein bands not migrating -
(reply: 3)
329.
Help with high background (have tried most trouble shooting) -
(reply: 8)
330.
Tumor protein precipitation at denaturing step? -
(reply: 1)
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