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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. aditional band on WB - (reply: 1)
302. Transferring >150 kDA proteins conditions - (reply: 3)
303. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
304. TAU gel electrophoresis of histones - (reply: 4)
305. Bands dark on sides and light in middle - (reply: 7)
306. Addition of sample loading buffer, before or after heating - (reply: 5)
307. question about protein expression normalization. - (reply: 6)
308. Protein bands not migrating - (reply: 3)
309. Help with high background (have tried most trouble shooting) - (reply: 8)
310. Tumor protein precipitation at denaturing step? - (reply: 1)
311. How to prepare blood serum for western blot? - (reply: 3)
312. TNF alpha and IL 1b Western Blot - (reply: 1)
313. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
314. SDS-PAGE trouble with protein concentration - (reply: 2)
315. Acrylamid - (reply: 5)
316. Detecting doublet bands in Western for eGFP control - (reply: 1)
317. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
318. Protein Size 50 kDa higher than expected - (reply: 3)
319. Acid urea gel electrophoresis (AUGE) - (reply: 7)
320. Phospho-protein vs total protein Western blot - (reply: 5)
321. storing the loaded gel before running it - (reply: 7)
322. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
323. Western blot trouble shooting - (reply: 3)
324. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
325. two bands on WB-alternative use of start codons? - (reply: 2)
326. TCA Precipitation to remove albumin - (reply: 6)
327. adjusting pH of 4x stacking buffer - (reply: 3)
328. No WB bands for H2AX at all...! - (reply: 10)
329. any tips for doing Fluorescence Western Blot? - (reply: 2)
330. Unknown silver/grey residue left after transfer. - (reply: 2)

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