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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. Good loading control for native gels? - (reply: 1)
302. Please help with odd bands and smears on Western blot - (reply: 4)
303. How to load equal amount of protein without using spectramax - (reply: 4)
304. Western blot membrane imaging - (reply: 7)
305. Detection of T7 RNA polymerase by WB - (reply: 6)
306. Shift in the position of band onto the western blot - (reply: 3)
307. Ghost and inverse bands and high background. Help! - (reply: 3)
308. No resolving of protein/bands below 50kDa :-s - (reply: 5)
309. Problems with wet transfer blot - (reply: 2)
310. 5% BSA blocking buffer - (reply: 3)
311. Storage of Membrane before addition of primary antibody - (reply: 3)
312. "Mickey Mouse" bands - (reply: 5)
313. SDS-PAGE : when the transfer stops - (reply: 1)
314. confusing Western results - (reply: 5)
315. aditional band on WB - (reply: 1)
316. Transferring >150 kDA proteins conditions - (reply: 3)
317. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
318. TAU gel electrophoresis of histones - (reply: 4)
319. Bands dark on sides and light in middle - (reply: 7)
320. Addition of sample loading buffer, before or after heating - (reply: 5)
321. question about protein expression normalization. - (reply: 6)
322. Protein bands not migrating - (reply: 3)
323. Help with high background (have tried most trouble shooting) - (reply: 8)
324. Tumor protein precipitation at denaturing step? - (reply: 1)
325. How to prepare blood serum for western blot? - (reply: 3)
326. TNF alpha and IL 1b Western Blot - (reply: 1)
327. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
328. SDS-PAGE trouble with protein concentration - (reply: 2)
329. Acrylamid - (reply: 5)
330. Detecting doublet bands in Western for eGFP control - (reply: 1)

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