Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
302. TAU gel electrophoresis of histones - (reply: 4)
303. Bands dark on sides and light in middle - (reply: 7)
304. Addition of sample loading buffer, before or after heating - (reply: 5)
305. question about protein expression normalization. - (reply: 6)
306. Protein bands not migrating - (reply: 3)
307. Help with high background (have tried most trouble shooting) - (reply: 8)
308. Tumor protein precipitation at denaturing step? - (reply: 1)
309. How to prepare blood serum for western blot? - (reply: 3)
310. TNF alpha and IL 1b Western Blot - (reply: 1)
311. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
312. SDS-PAGE trouble with protein concentration - (reply: 2)
313. Acrylamid - (reply: 5)
314. Detecting doublet bands in Western for eGFP control - (reply: 1)
315. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
316. Protein Size 50 kDa higher than expected - (reply: 3)
317. Acid urea gel electrophoresis (AUGE) - (reply: 7)
318. Phospho-protein vs total protein Western blot - (reply: 5)
319. storing the loaded gel before running it - (reply: 7)
320. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
321. Western blot trouble shooting - (reply: 3)
322. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
323. two bands on WB-alternative use of start codons? - (reply: 2)
324. TCA Precipitation to remove albumin - (reply: 6)
325. adjusting pH of 4x stacking buffer - (reply: 3)
326. No WB bands for H2AX at all...! - (reply: 10)
327. any tips for doing Fluorescence Western Blot? - (reply: 2)
328. Unknown silver/grey residue left after transfer. - (reply: 2)
329. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
330. Changes to housekeeping loading controls after drug treatment - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36