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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. SDS-PAGE : when the transfer stops - (reply: 1)
302. confusing Western results - (reply: 5)
303. aditional band on WB - (reply: 1)
304. Transferring >150 kDA proteins conditions - (reply: 3)
305. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
306. TAU gel electrophoresis of histones - (reply: 4)
307. Bands dark on sides and light in middle - (reply: 7)
308. Addition of sample loading buffer, before or after heating - (reply: 5)
309. question about protein expression normalization. - (reply: 6)
310. Protein bands not migrating - (reply: 3)
311. Help with high background (have tried most trouble shooting) - (reply: 8)
312. Tumor protein precipitation at denaturing step? - (reply: 1)
313. How to prepare blood serum for western blot? - (reply: 3)
314. TNF alpha and IL 1b Western Blot - (reply: 1)
315. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
316. SDS-PAGE trouble with protein concentration - (reply: 2)
317. Acrylamid - (reply: 5)
318. Detecting doublet bands in Western for eGFP control - (reply: 1)
319. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
320. Protein Size 50 kDa higher than expected - (reply: 3)
321. Acid urea gel electrophoresis (AUGE) - (reply: 7)
322. Phospho-protein vs total protein Western blot - (reply: 5)
323. storing the loaded gel before running it - (reply: 7)
324. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
325. Western blot trouble shooting - (reply: 3)
326. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
327. two bands on WB-alternative use of start codons? - (reply: 2)
328. TCA Precipitation to remove albumin - (reply: 6)
329. adjusting pH of 4x stacking buffer - (reply: 3)
330. No WB bands for H2AX at all...! - (reply: 10)

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