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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. Shift in the position of band onto the western blot - (reply: 3)
302. Ghost and inverse bands and high background. Help! - (reply: 3)
303. No resolving of protein/bands below 50kDa :-s - (reply: 5)
304. Problems with wet transfer blot - (reply: 2)
305. 5% BSA blocking buffer - (reply: 3)
306. Storage of Membrane before addition of primary antibody - (reply: 3)
307. "Mickey Mouse" bands - (reply: 5)
308. SDS-PAGE : when the transfer stops - (reply: 1)
309. confusing Western results - (reply: 5)
310. aditional band on WB - (reply: 1)
311. Transferring >150 kDA proteins conditions - (reply: 3)
312. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
313. TAU gel electrophoresis of histones - (reply: 4)
314. Bands dark on sides and light in middle - (reply: 7)
315. Addition of sample loading buffer, before or after heating - (reply: 5)
316. question about protein expression normalization. - (reply: 6)
317. Protein bands not migrating - (reply: 3)
318. Help with high background (have tried most trouble shooting) - (reply: 8)
319. Tumor protein precipitation at denaturing step? - (reply: 1)
320. How to prepare blood serum for western blot? - (reply: 3)
321. TNF alpha and IL 1b Western Blot - (reply: 1)
322. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
323. SDS-PAGE trouble with protein concentration - (reply: 2)
324. Acrylamid - (reply: 5)
325. Detecting doublet bands in Western for eGFP control - (reply: 1)
326. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
327. Protein Size 50 kDa higher than expected - (reply: 3)
328. Acid urea gel electrophoresis (AUGE) - (reply: 7)
329. Phospho-protein vs total protein Western blot - (reply: 5)
330. storing the loaded gel before running it - (reply: 7)

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