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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. SDS Page and WB: Wrong size bands - (reply: 2)
302. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
303. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
304. How to avoid gel breakage after electrophoresis - (reply: 5)
305. What is an Input in SDS-PAGE? - (reply: 1)
306. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
307. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
308. Good loading control for native gels? - (reply: 1)
309. Please help with odd bands and smears on Western blot - (reply: 4)
310. How to load equal amount of protein without using spectramax - (reply: 4)
311. Western blot membrane imaging - (reply: 7)
312. Detection of T7 RNA polymerase by WB - (reply: 6)
313. Shift in the position of band onto the western blot - (reply: 3)
314. Ghost and inverse bands and high background. Help! - (reply: 3)
315. No resolving of protein/bands below 50kDa :-s - (reply: 5)
316. Problems with wet transfer blot - (reply: 2)
317. 5% BSA blocking buffer - (reply: 3)
318. Storage of Membrane before addition of primary antibody - (reply: 3)
319. "Mickey Mouse" bands - (reply: 5)
320. SDS-PAGE : when the transfer stops - (reply: 1)
321. confusing Western results - (reply: 5)
322. aditional band on WB - (reply: 1)
323. Transferring >150 kDA proteins conditions - (reply: 3)
324. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
325. TAU gel electrophoresis of histones - (reply: 4)
326. Bands dark on sides and light in middle - (reply: 7)
327. Addition of sample loading buffer, before or after heating - (reply: 5)
328. question about protein expression normalization. - (reply: 6)
329. Protein bands not migrating - (reply: 3)
330. Help with high background (have tried most trouble shooting) - (reply: 8)

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