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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
301. Problems with protein estimation and western blots - (reply: 1)
302. Loading controls - (reply: 1)
303. Quantity of protein to charge - (reply: 1)
304. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
305. Tris-Hcl becomes cloudy. - (reply: 3)
306. Can FLAG IP, but cannot detect with Western - (reply: 4)
307. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
308. Redoing only the Secondary Antibody staining on a Western - (reply: 1)
309. Native PAGE, interaction of proteins, validity - (reply: 4)
310. Smeared transfer on nitrocellulose membrane - (reply: 2)
311. blocking and primary antibody in different buffers? - (reply: 1)
312. Same non specific band for different target proteins - (reply: 1)
313. Types of 96-well plates used for BCA protein quantification - (reply: 1)
314. Dot blot halo - (reply: 4)
315. help: i accidentally use transfer buffer as running buffer, what will happen??? - (reply: 4)
316. Western blot and isoelectric point - (reply: 1)
317. Western Blot Developing Cassette - (reply: 1)
318. Pull down assay and Co- IP experiment - (reply: 6)
319. Trouble detecting Histaq - (reply: 4)
320. Western gels running super slow! - (reply: 3)
321. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
322. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
323. WB parametric or non-parametric - (reply: 6)
324. Trouble with the GFP signal - (reply: 1)
325. Intern Needing Help - (reply: 3)
326. SDS-Electrophoresis, Dye color change - (reply: 1)
327. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
328. Trouble with equal loading - (reply: 8)
329. how many times striping can b done of the blot - (reply: 9)
330. SDS-PAGE of negatively charged protein - (reply: 4)

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